Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104850 |
| Name | oriT_p1_1801121  |
| Organism | Staphylococcus argenteus strain 1801221 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP083806 (6680..6715 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_p1_1801121
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3436 | GenBank | WP_012896701 |
| Name | MobA_MobL_LB317_RS13405_p1_1801121 |
UniProt ID | D2KNY7 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78929.92 Da Isoelectric Point: 5.5367
>WP_012896701.1 MULTISPECIES: MobA/MobL family protein [Staphylococcus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNSYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGQKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYEESERLYDLKQKTALITDDEDRLNKIENIEDRQRKLESINEVFEKQSKIFFDKNYPDQKLNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGSENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVLGAIQKKLLSEY
DFDYSDNNDLKHLYQESYEVGDEISKDNIEEFYEGNDILVDKETYNNYNKKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNSYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGQKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYEESERLYDLKQKTALITDDEDRLNKIENIEDRQRKLESINEVFEKQSKIFFDKNYPDQKLNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGSENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVLGAIQKKLLSEY
DFDYSDNNDLKHLYQESYEVGDEISKDNIEEFYEGNDILVDKETYNNYNKKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | D2KNY7 |
T4CP
| ID | 3378 | GenBank | WP_012896715 |
| Name | t4cp2_LB317_RS13495_p1_1801121 |
UniProt ID | _ |
| Length | 548 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 548 a.a. Molecular weight: 62950.94 Da Isoelectric Point: 7.1940
>WP_012896715.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYARYEFEPEKRTMGNIIKFIQNNKPNQDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYLDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQERIKQQKLEEYKEEQ
RQKELEEEQQPQEEQQNKKVKESKDKNNEPQEDKKDEQQKINESKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYARYEFEPEKRTMGNIIKFIQNNKPNQDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYLDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQERIKQQKLEEYKEEQ
RQKELEEEQQPQEEQQNKKVKESKDKNNEPQEDKKDEQQKINESKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 22627..34613
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LB317_RS13450 (LB317_13460) | 18779..19525 | - | 747 | WP_001081004 | class I SAM-dependent methyltransferase | - |
| LB317_RS13455 (LB317_13465) | 19658..20332 | + | 675 | WP_012896694 | IS6-like element IS257 family transposase | - |
| LB317_RS13460 (LB317_13470) | 20397..20615 | + | 219 | WP_238839093 | YolD-like family protein | - |
| LB317_RS13465 (LB317_13475) | 20657..21928 | - | 1272 | WP_012896721 | hypothetical protein | - |
| LB317_RS13470 (LB317_13480) | 21997..22611 | - | 615 | WP_012896720 | hypothetical protein | - |
| LB317_RS13475 (LB317_13485) | 22627..23250 | - | 624 | WP_061739436 | hypothetical protein | traP |
| LB317_RS13480 (LB317_13490) | 23287..24018 | - | 732 | WP_012896718 | hypothetical protein | - |
| LB317_RS13485 (LB317_13495) | 24075..24467 | - | 393 | WP_012896717 | single-stranded DNA-binding protein | - |
| LB317_RS13490 (LB317_13500) | 24484..25401 | - | 918 | WP_012896716 | conjugal transfer protein TrbL family protein | trsL |
| LB317_RS13495 (LB317_13505) | 25479..27125 | - | 1647 | WP_012896715 | VirD4-like conjugal transfer protein, CD1115 family | - |
| LB317_RS13500 (LB317_13510) | 27122..27586 | - | 465 | WP_012896714 | hypothetical protein | trsJ |
| LB317_RS13505 (LB317_13515) | 27602..29704 | - | 2103 | WP_012896713 | DNA topoisomerase III | - |
| LB317_RS13510 (LB317_13520) | 29720..30205 | - | 486 | WP_012896712 | TrsH/TraH family protein | - |
| LB317_RS13515 (LB317_13525) | 30215..31291 | - | 1077 | WP_012896711 | CHAP domain-containing protein | trsG |
| LB317_RS13520 (LB317_13530) | 31309..32589 | - | 1281 | WP_012896710 | conjugal transfer protein TraF | trsF |
| LB317_RS13525 (LB317_13535) | 32601..34613 | - | 2013 | WP_061739437 | DUF87 domain-containing protein | virb4 |
Host bacterium
| ID | 5288 | GenBank | NZ_CP083806 |
| Plasmid name | p1_1801121 | Incompatibility group | - |
| Plasmid size | 34870 bp | Coordinate of oriT [Strand] | 6680..6715 [-] |
| Host baterium | Staphylococcus argenteus strain 1801221 |
Cargo genes
| Drug resistance gene | mupA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |