Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104835 |
| Name | oriT_CriePir120|unnamed2  |
| Organism | Klebsiella pneumoniae strain CriePir120 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP063010 (60807..60912 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_CriePir120|unnamed2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3425 | GenBank | WP_050868819 |
| Name | Relaxase_GJJ08_RS27735_CriePir120|unnamed2 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 3364 | GenBank | WP_011091092 |
| Name | t4cp2_GJJ08_RS27445_CriePir120|unnamed2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 63708..82811
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GJJ08_RS27715 (GJJ08_027715) | 58792..59103 | + | 312 | WP_011091065 | hypothetical protein | - |
| GJJ08_RS27720 (GJJ08_027720) | 59236..59772 | + | 537 | WP_011091066 | hypothetical protein | - |
| GJJ08_RS27725 (GJJ08_027725) | 60273..60638 | - | 366 | WP_011091067 | hypothetical protein | - |
| GJJ08_RS28080 | 60653..61231 | + | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| GJJ08_RS27735 (GJJ08_027735) | 61218..63197 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| GJJ08_RS27740 (GJJ08_027740) | 63211..63711 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| GJJ08_RS27745 (GJJ08_027745) | 63708..64487 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| GJJ08_RS27750 (GJJ08_027750) | 64498..65661 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| GJJ08_RS27755 (GJJ08_027755) | 65651..65911 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| GJJ08_RS28085 | 65936..69148 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| GJJ08_RS27765 (GJJ08_027765) | 69114..69626 | + | 513 | WP_011091071 | hypothetical protein | traL |
| GJJ08_RS27770 (GJJ08_027770) | 69627..69839 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| GJJ08_RS27775 (GJJ08_027775) | 69811..70221 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| GJJ08_RS28090 | 70289..70495 | + | 207 | WP_227659897 | hypothetical protein | traM |
| GJJ08_RS28095 | 70547..71002 | + | 456 | WP_264755453 | DotI/IcmL family type IV secretion protein | traM |
| GJJ08_RS27785 (GJJ08_027785) | 71011..72162 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| GJJ08_RS27790 (GJJ08_027790) | 72174..73523 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| GJJ08_RS27795 (GJJ08_027795) | 73535..74239 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| GJJ08_RS27800 (GJJ08_027800) | 74263..74793 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| GJJ08_RS27805 (GJJ08_027805) | 74810..75199 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| GJJ08_RS27810 (GJJ08_027810) | 75245..75739 | + | 495 | WP_011091080 | hypothetical protein | - |
| GJJ08_RS27815 (GJJ08_027815) | 75736..78786 | + | 3051 | WP_011091081 | hypothetical protein | traU |
| GJJ08_RS27820 (GJJ08_027820) | 78783..79991 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| GJJ08_RS27825 (GJJ08_027825) | 79988..80638 | + | 651 | WP_011091083 | hypothetical protein | - |
| GJJ08_RS27830 (GJJ08_027830) | 80631..82811 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| GJJ08_RS27835 (GJJ08_027835) | 82814..83467 | + | 654 | WP_011091084 | hypothetical protein | - |
| GJJ08_RS27840 (GJJ08_027840) | 83542..83772 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 5273 | GenBank | NZ_CP063010 |
| Plasmid name | CriePir120|unnamed2 | Incompatibility group | IncL/M |
| Plasmid size | 84069 bp | Coordinate of oriT [Strand] | 60807..60912 [+] |
| Host baterium | Klebsiella pneumoniae strain CriePir120 |
Cargo genes
| Drug resistance gene | blaTEM-1B, aadA5, qacE, sul1, armA, msr(E), mph(E), blaCTX-M-15 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |