Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104811
• Name: oriT_pP18_g
• Organism: Phaeobacter piscinae strain P18
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP010722 (33816..33835 [+], 20 nt)
• oriT length: 20 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 20 nt

>oriT_pP18_g
GTTTGCATAAGTGCGCCCTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_phaeop18_RS20785_pP18_g

• ID: 3410
• GenBank: WP_067267002
• Name: mobQ_phaeop18_RS20785_pP18_g
• UniProt ID: A0A167GVD0
• Length: 430 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 430 a.a.
• Molecular weight: 48835.92 Da
• Isoelectric Point: 6.9896

>WP_067267002.1 MULTISPECIES: MobQ family relaxase [Roseobacteraceae]
MASYHLSVKTIKRSAGRSATAAAAYRVGERIECQREGRVHDYTRKQGIEETFILAPKDAPDWATDRSRLW
NEVEARETRRNSVTAREWELALPSEISAEDRSQITRDFAQELVSRYGVAVDVAIHAPHREGDQRNHHAHV
LTSTRRLEPEGFTAKTRVLDSAKTGGVEIEQMRGLWAELQNRALERAGEVERVDHRSLEAQREAAQERGD
KLSAEELDRDPELKLGPAANSMERRAKAMAERQGREYKPVTERGAVVHAARQARAAFREMRARLELARET
YGIEREAGQGRVSAGLAALRAATAKDHDRDRNPDDFRERLARVLGRSRDQDDTPKPEGRNYARERLKEIM
EKDAGRDGQAAVHKLDGHSEYDLGEETGRAARKPSVNERLKDVLNKPRGRLEIEDEREQERGDDENENTR
DKDRGEGHSL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-202)

Protein structure

• AlphaFold DB: A0A167GVD0

T4CP

t4cp2_phaeop18_RS20720_pP18_g

• ID: 3348
• GenBank: WP_071974173
• Name: t4cp2_phaeop18_RS20720_pP18_g
• UniProt ID: _
• Length: 309 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 309 a.a.
• Molecular weight: 34655.54 Da
• Isoelectric Point: 5.4963

>WP_071974173.1 MULTISPECIES: nucleotidyl transferase AbiEii/AbiGii toxin family protein [Roseobacteraceae]
MSDNDPHAQDRTFDIVDVDVRAWVETARANPTLYRDRQVTEIVLGAIGLAPSLSKTLVLKGGAVMALAFK
SNRVTGDVDFTSMAEPADLTEKITAELNEILPSTAIKLGYLDLICRVQSVKKMPRAENFEDHEFPALKVK
VGSATRGTAEAARLADGKASRVLDVEISFRDQVYAFQELNLHGAGVAVRAFTIHELIAEKFRALLQQITR
KHKRNRRQDVYDIAFLIDEHDFGDADKTAILTTFIEKCRSRGIEVKPDSMDDPEIKQRAQADWETLALEI
GDLPPFEERFALMRDLYVSLPWGGIKKDR

Protein domains

Predicted by InterproScan.

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (AbiEii) (51-285)

Protein structure

No available structure.

t4cp2_phaeop18_RS20800_pP18_g

• ID: 3349
• GenBank: WP_096873673
• Name: t4cp2_phaeop18_RS20800_pP18_g
• UniProt ID: _
• Length: 556 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 556 a.a.
• Molecular weight: 61380.31 Da
• Isoelectric Point: 6.8841

>WP_096873673.1 type IV secretory system conjugative DNA transfer family protein [Phaeobacter piscinae]
MRSVMLVFGGLFRFFGRLIFTPIMLGWLIGAMAFGAMLGTLVATPFIFTFFDQTPGESAWQWLVFGPFMF
VGAVFGFQYWRMASGADAYFGLTGDSHGSARFANRKELKKLERSKGQDDAGLLIGRNPHTGRLLRYDGPA
HLITLAPTRAGKGVGTVIPNLLAAERSVLVIDPKGENARIAGEARRRFGTVHVLDPFEVSGHPSACYNPL
DRLTPHSLDLGEDAASLTEALVMDPPGQVTEAHWNEEAKAILGGLIMFCVCHEDRDRRSLATVREYLTLP
PEKLRALLELMQDSDEAGGLIARAANRFLGKADREAASVLSNAQRHTHFLDSPRIAKCLARSDFAFSDLR
HRITSVFLVLPPNRMDAYSRWLRLLVSQALQDIARDAERPQNASEGRSERLTAPALFLLDEFAALGRLEA
VERAMGLMAGYGLQLWPILQDMSQLKDLYGERAGTFIANAGVQQVFGVNDFETAKWLSQMIGQETAGFQT
DSFKPGDGPSFSNNLTGRDLLTPDEIMQLPPKRQLLRVQGQATAVAQKLRYYADPEFAGLFTPETP

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (116-547)

Protein structure

No available structure.

Host bacterium

• ID: 5249
• GenBank: NZ_CP010722
• Plasmid name: pP18_g
• Incompatibility group: -
• Plasmid size: 36658 bp
• Coordinate of oriT [Strand]: 33816..33835 [+]
• Host baterium: Phaeobacter piscinae strain P18

Cargo genes

• Drug resistance gene: aph(6)-Id, aph(3'')-Ib
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -