Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104803
• Name: oriT_pC02
• Organism: Staphylococcus aureus strain USA300_2014.C02
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP012121 ( 42430..42669 [+], 240 nt)
• oriT length: 240 nt
• IRs (inverted repeats): 171..178, 183..190 (TACCCATT..AATGGGTA) 154..160, 164..170 (ATCTGGC..GCCAGAT) 87..93, 98..104 (TGTCACA..TGTGACA) 55..61, 66..72 (TGTCACA..TGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 240 nt

>oriT_pC02
GAAATATACACAATAATAGGGGTGGAACACTTTTGTGACAATTTCAATATATTGTGTCACAAAAGTGTGACAATTTCAATATATTGTGTCACAAAAGTGTGACAATTTAATTTTTGTGACCCCCATAAGATAAATGTTTAAAACCCTTGAAATATCTGGCTTCGCCAGATTACCCATTATAAAATGGGTACATATTTCCCTTATGCTCTTACAAATATCTAGCTATATTAGGAGGTAATA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_ACO02_RS15055_pC02

• ID: 3406
• GenBank: WP_031770318
• Name: mobP2_ACO02_RS15055_pC02
• UniProt ID: _
• Length: 391 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 391 a.a.
• Molecular weight: 47036.61 Da
• Isoelectric Point: 8.9143

>WP_031770318.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MPKIVTMSEFEQPQNKSNYGNYVNYVDREEAKKNEHEVFQYDVYAHYMFDEQKSNSMFNENSNYMNKNEI
EYTKTKFNEAQKNNGIMWKDVISFDNDSLKEAGIFDPDCKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYNTEMKKQLKKIHEEVKDIPKKEWQYNNNIMKHVRPEIDKFTEMYIKNNHGEEFESFKQDLKKQSK
LYKETYGDKSEYKKYEETKMNDLYSRAGNTILKQLKSFNEEYNGIKYKEPNKLDNKNIPNYNPKATSKMQ
IGYALNNTLYNTQRALRNDFQKERNINQYHYEFDKSYQPEQ

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-363)

Protein structure

No available structure.

T4CP

t4cp2_ACO02_RS15005_pC02

• ID: 3342
• GenBank: WP_223289527
• Name: t4cp2_ACO02_RS15005_pC02
• UniProt ID: _
• Length: 941 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 941 a.a.
• Molecular weight: 108591.55 Da
• Isoelectric Point: 7.4547

>WP_223289527.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MIPFRSKHNHGYHRKKASFSQNKHLLAHPIGLLALLVVGFIFIMLAANFFINMLNNLIFAGGDIFDNMKK
INNEWTNYFFKLRFDLALFYIPFFVLLTVLWGKKVYQIRQKFKSLDNQEKASGRFSTKKEIKQQYKAIAQ
REKQFEGEGGLPVAMFGVYDFATKTKQYLEQRKTAKTEINSMPDLTQDEKYNMKKDKLGQINKEFVSTFF
LKREFTFIDESPTNNIFVGTSRSGKGEVFVLSMIENYSRSSHQPSLVVGDMKGELYSASNETLENRNYHT
EVFNLDQPMESTMSFNLLQLVIEEYQKGDLGEAQEMTKQITHTLTNNEGVEDNEWNLMSAALINAMILAL
CEEALPEHPEKVTLYSVSNMLQTLSVKKFYKQIQIGNQVKVIETSALDEYMERFPSHSPAKTQYATVEAA
PDKMRSSIIGTALKALQPFTTDTIAKLTSHSSLDLNKLGFPSIIDGFSEPDSKFELNVQVKRETNIGTKY
DEVEGINIGVNEYGYWQYPFKTVLNIDDRIEIVETDEFNEEVKYYFYVTSIDKKGQVTFEAKGDIYSSNV
TIRKFNSFAKPIAIFMVVPDYNSANHGIASILVNQIVFELSKNSQLYTEKQSTHRRVIFHLDEAGNMPQI
PNLSQKVNVSLSRGLRFNFFVQAFSQIKDTYGDAYDAIMDACQNKVFIMATQEQTLKDFSDMIGSQQITV
RSRSGETDSLKSSITENQEERPLLRADELAHLQEGETVVVRNLKRQDNKRRKVMSYPIFNSGKYAMKYRY
QYLADLMDTGKSIIHFRPLLKERCEHRHLQLETTLVDWEKIIENMQAGIENDSNNDIDDLTQSLNKSMGE
KANANNPEAYKEENNKKPVTRQKKVLTLKAQLSAEEFDRLTRRFTKTIRMYEEDGNNVDRLTLYKLKQYL
KLRVRNNDITEEQKNKSINYVEQLIEEAKAK

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (616-733)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (190-459)

Protein structure

No available structure.

Host bacterium

• ID: 1491
• GenBank: NZ_CP012121
• Plasmid name: pC02
• Incompatibility group: -
• Plasmid size: 61539 bp
• Coordinate of oriT [Strand]: 10415..10654 [+]; 42430..42669 [+]
• Host baterium: Staphylococcus aureus strain USA300_2014.C02

Cargo genes

• Drug resistance gene: msr(A), mph(C), qacA, blaZ
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21