Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104796
Name   oriT_IncAC2 in_silico
Organism   Klebsiella pneumoniae strain 1_GR_13
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP027043 (20269..20419 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_IncAC2
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3403 GenBank   WP_001337757
Name   mobH_A6D67_RS25755_IncAC2 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   3338 GenBank   WP_000178856
Name   traD_A6D67_RS25760_IncAC2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   3339 GenBank   WP_000637386
Name   traC_A6D67_RS25835_IncAC2 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18353..43544

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D67_RS25745 (A6D67_025925) 14378..14611 - 234 WP_001191892 hypothetical protein -
A6D67_RS25750 (A6D67_025930) 14593..15210 - 618 WP_001249396 hypothetical protein -
A6D67_RS25755 (A6D67_025935) 15378..18356 + 2979 WP_001337757 MobH family relaxase -
A6D67_RS25760 (A6D67_025940) 18353..20218 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
A6D67_RS25765 (A6D67_025945) 20268..20813 + 546 WP_000228720 hypothetical protein -
A6D67_RS25770 (A6D67_025950) 20770..21399 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
A6D67_RS25775 (A6D67_025955) 21409..21855 + 447 WP_000122506 hypothetical protein -
A6D67_RS25780 (A6D67_025960) 21865..22242 + 378 WP_000869261 hypothetical protein -
A6D67_RS25785 (A6D67_025965) 22242..22904 + 663 WP_001231463 hypothetical protein -
A6D67_RS29810 23085..23228 + 144 WP_001275801 hypothetical protein -
A6D67_RS25790 (A6D67_025970) 23240..23605 + 366 WP_001052531 hypothetical protein -
A6D67_RS25795 (A6D67_025975) 23750..24031 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
A6D67_RS25800 (A6D67_025980) 24028..24654 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
A6D67_RS25805 (A6D67_025985) 24638..25555 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
A6D67_RS25810 (A6D67_025990) 25555..26868 + 1314 WP_024131605 TraB/VirB10 family protein traB
A6D67_RS25815 (A6D67_025995) 26865..27443 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
A6D67_RS25820 (A6D67_026000) 27447..27839 + 393 WP_000479535 TraA family conjugative transfer protein -
A6D67_RS25825 (A6D67_026005) 28040..33571 + 5532 WP_000606833 Ig-like domain-containing protein -
A6D67_RS25830 (A6D67_026010) 33720..34427 + 708 WP_001259347 DsbC family protein -
A6D67_RS25835 (A6D67_026015) 34424..36871 + 2448 WP_000637386 type IV secretion system protein TraC virb4
A6D67_RS25840 (A6D67_026020) 36886..37203 + 318 WP_000351984 hypothetical protein -
A6D67_RS25845 (A6D67_026025) 37200..37730 + 531 WP_001010738 S26 family signal peptidase -
A6D67_RS25850 (A6D67_026030) 37693..38958 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
A6D67_RS25855 (A6D67_026035) 38955..39626 + 672 WP_001337754 EAL domain-containing protein -
A6D67_RS25860 (A6D67_026040) 39626..40630 + 1005 WP_043940352 TraU family protein traU
A6D67_RS25865 (A6D67_026045) 40746..43544 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
A6D67_RS25870 (A6D67_026050) 43583..44443 - 861 WP_000709517 hypothetical protein -
A6D67_RS25875 (A6D67_026055) 44566..45207 - 642 WP_000796664 hypothetical protein -
A6D67_RS25880 (A6D67_026060) 45501..45821 + 321 WP_000547566 hypothetical protein -
A6D67_RS25885 (A6D67_026065) 46125..46310 + 186 WP_001186917 hypothetical protein -
A6D67_RS25890 (A6D67_026070) 46530..47498 + 969 WP_000085162 AAA family ATPase -
A6D67_RS25895 (A6D67_026075) 47509..48417 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   5235 GenBank   NZ_CP027043
Plasmid name   IncAC2 Incompatibility group   IncA/C2
Plasmid size   192771 bp Coordinate of oriT [Strand]   20269..20419 [+]
Host baterium   Klebsiella pneumoniae strain 1_GR_13

Cargo genes

Drug resistance gene   sul2, dfrA14, dfrA23, blaVEB-1, ant(2'')-Ia, ARR-3, cmlA1, blaOXA-10, ant(3'')-Ia, qacE, floR, tet(G), rmtB, blaTEM-1B, tet(A), aph(6)-Id, aph(3'')-Ib
Virulence gene   -
Metal resistance gene   fecD, fecE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -