Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104763
Name   oriT_p19-10_02 in_silico
Organism   Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP023489 (26741..26790 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_p19-10_02
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3390 GenBank   WP_057182142
Name   traI_AN663_RS29505_p19-10_02 insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190600.69 Da        Isoelectric Point: 6.3089

>WP_057182142.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKAAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDTAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWADPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASEGLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1433-1555)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

TrwC relaxase (TrwC)

(10-284)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

AAA domain (AAA_30)

(971-1157)


  Protein structure



No available structure.




T4CP

ID   3329 GenBank   WP_031944110
Name   traD_AN663_RS29500_p19-10_02 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86032.08 Da        Isoelectric Point: 5.0175

>WP_031944110.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKRPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDEVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 26183..54603

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN663_RS29280 (AN663_29060) 21874..22200 + 327 WP_004152639 hypothetical protein -
AN663_RS29285 (AN663_29065) 22256..22630 + 375 WP_004152638 hypothetical protein -
AN663_RS31180 22800..22952 + 153 Protein_29 DUF5431 family protein -
AN663_RS29295 (AN663_29075) 22897..23019 + 123 WP_085842394 Hok/Gef family protein -
AN663_RS29300 (AN663_29080) 23719..23991 + 273 WP_004152759 hypothetical protein -
AN663_RS29305 (AN663_29085) 23988..24338 + 351 WP_004152758 hypothetical protein -
AN663_RS29315 (AN663_29100) 24967..25788 + 822 WP_004178064 DUF932 domain-containing protein -
AN663_RS29320 (AN663_29105) 25821..26150 + 330 WP_011977736 DUF5983 family protein -
AN663_RS29325 (AN663_29110) 26183..26668 - 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
AN663_RS29330 (AN663_29115) 27102..27494 + 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
AN663_RS29335 (AN663_29120) 27708..28403 + 696 WP_004178061 transcriptional regulator TraJ family protein -
AN663_RS29340 (AN663_29125) 28487..28858 + 372 WP_004208838 TraY domain-containing protein -
AN663_RS29345 (AN663_29130) 28912..29280 + 369 WP_004178060 type IV conjugative transfer system pilin TraA -
AN663_RS29350 (AN663_29135) 29294..29599 + 306 WP_004178059 type IV conjugative transfer system protein TraL traL
AN663_RS29355 (AN663_29140) 29619..30185 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
AN663_RS29360 (AN663_29145) 30172..30912 + 741 WP_004152601 type-F conjugative transfer system secretin TraK traK
AN663_RS29365 (AN663_29150) 30912..32336 + 1425 WP_004152600 F-type conjugal transfer pilus assembly protein TraB traB
AN663_RS29370 (AN663_29155) 32329..32925 + 597 WP_004152599 conjugal transfer pilus-stabilizing protein TraP -
AN663_RS29375 (AN663_29160) 32948..33517 + 570 WP_004152598 type IV conjugative transfer system lipoprotein TraV traV
AN663_RS29380 (AN663_29165) 33649..34059 + 411 WP_004152597 lipase chaperone -
AN663_RS29385 (AN663_29170) 34064..34354 + 291 WP_004152596 hypothetical protein -
AN663_RS29390 (AN663_29175) 34378..34596 + 219 WP_004171484 hypothetical protein -
AN663_RS29395 (AN663_29180) 34597..34914 + 318 WP_004152595 hypothetical protein -
AN663_RS29400 (AN663_29185) 34981..35385 + 405 WP_031944109 hypothetical protein -
AN663_RS29410 (AN663_29195) 35681..36079 + 399 WP_004153071 hypothetical protein -
AN663_RS29415 (AN663_29200) 36151..38790 + 2640 WP_004152592 type IV secretion system protein TraC virb4
AN663_RS29420 (AN663_29205) 38790..39179 + 390 WP_004152591 type-F conjugative transfer system protein TrbI -
AN663_RS29425 (AN663_29210) 39179..39805 + 627 WP_004152590 type-F conjugative transfer system protein TraW traW
AN663_RS29430 (AN663_29215) 39849..40808 + 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
AN663_RS29435 (AN663_29220) 40821..41459 + 639 WP_004152682 type-F conjugative transfer system pilin assembly protein TrbC trbC
AN663_RS29440 (AN663_29225) 41507..43462 + 1956 WP_004153093 type-F conjugative transfer system mating-pair stabilization protein TraN traN
AN663_RS29445 (AN663_29230) 43494..43730 + 237 WP_004152683 conjugal transfer protein TrbE -
AN663_RS31365 (AN663_29235) 43727..43911 + 185 Protein_59 hypothetical protein -
AN663_RS29455 (AN663_29240) 43957..44283 + 327 WP_004152685 hypothetical protein -
AN663_RS29460 (AN663_29245) 44304..45056 + 753 WP_004152686 type-F conjugative transfer system pilin assembly protein TraF traF
AN663_RS29465 (AN663_29250) 45067..45306 + 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
AN663_RS29470 (AN663_29255) 45278..45850 + 573 WP_004152688 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
AN663_RS29475 (AN663_29260) 45843..46271 + 429 WP_004152689 hypothetical protein -
AN663_RS29480 (AN663_29265) 46258..47628 + 1371 WP_004178055 conjugal transfer pilus assembly protein TraH traH
AN663_RS29485 (AN663_29270) 47628..50477 + 2850 WP_004178054 conjugal transfer mating-pair stabilization protein TraG traG
AN663_RS29490 (AN663_29275) 50483..51010 + 528 WP_004153030 hypothetical protein -
AN663_RS29495 (AN663_29280) 51200..51931 + 732 WP_004152629 conjugal transfer complement resistance protein TraT -
AN663_RS29500 (AN663_29285) 52291..54603 + 2313 WP_031944110 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   5202 GenBank   NZ_CP023489
Plasmid name   p19-10_02 Incompatibility group   IncFII
Plasmid size   124323 bp Coordinate of oriT [Strand]   26741..26790 [-]
Host baterium   Klebsiella pneumoniae subsp. pneumoniae strain ST101:960186733

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9