Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104749
• Name: oriT_pUSA08-1-SUR24
• Organism: Staphylococcus aureus strain USA300-SUR24
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP014448 (20791..20826 [+], 36 nt)
• oriT length: 36 nt
• IRs (inverted repeats): 4..10, 14..20 (GCGAACT..AGTTCGC)
• Location of nic site: 29..30
• Conserved sequence flanking the nic site: GTGCGCCCTT
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 36 nt

>oriT_pUSA08-1-SUR24
CACGCGAACTGAAAGTTCGCATAAGTGCGCCCTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_MobL_AYM37_RS15785_pUSA08-1-SUR24

• ID: 3383
• GenBank: WP_077444064
• Name: MobA_MobL_AYM37_RS15785_pUSA08-1-SUR24
• UniProt ID: _
• Length: 665 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 665 a.a.
• Molecular weight: 79052.11 Da
• Isoelectric Point: 5.8224

>WP_077444064.1 MobA/MobL family protein [Staphylococcus aureus]
MAMYHFQNKFVSKAKGQSATAKSAYNSASKIKDYKENEFKDYSNKQCDYSEILLPYNADEKFKDREYLWN
KVNEVENRKNSQVAREIIIGLPNEFDPKSNVELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWETVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSTISKHNEEIKKRNNSKIDKMFKDVEDIKSHKLNSFSYMQNTDSKILKDMAKDLKLFI
TPINIYKENERLYDLKQKTALISDDEDRLSKIEDIEDRQQKLESINNVFEKQAQIFFDKNYPDQTKDYDK
NEKIFITRTILNDRDDLPAHNELDDIIKDKREKEAQISLNTVLGNRDISLQSIEDESNFFTGKLSHILEE
NSLSFDDVLDNKHEGLDDEMKIDYYTNKLEVLRNAENTLEEYYDIQIKDLFTDNDDYRSFNEVTNIKEKQ
QLIDFKEYHGTENTLEMLETGQFIPKYNDEDRKYITESIKLLQEKEFKPNKSQHDTYVIGAIQKKLLKDF
DFDYSNNNDLKHLYQETNEVKDELSKGNIEEYYNGNDILIDKENFNNYQKKSQASNLINASVDSFIFNFN
EIFRERMPKYINKQYKGKNHSRKRHELKNKRGIHL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-216)

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (263-359)

Protein structure

No available structure.

T4CP

t4cp2_AYM37_RS15680_pUSA08-1-SUR24

• ID: 3318
• GenBank: WP_077444030
• Name: t4cp2_AYM37_RS15680_pUSA08-1-SUR24
• UniProt ID: _
• Length: 543 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 543 a.a.
• Molecular weight: 62085.11 Da
• Isoelectric Point: 8.3315

>WP_077444030.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MTTILGELDAKSVKQKKLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGELYE
KTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDASIVAELIIKSAGDSKINRDVWFKSSVGLLNS
LILYAKYEFPPEKRNMENVIKFVQNNKPKKESDDGSAELDKRFSELPKDHPARESYEFGFDVSEDRTRSS
IVLTFVSDLRNYVDREVRSYTSDSDFDLKDVGKQKTIVYVMLPVLGNSNTALSSLFFSQMFQQLYRLGDD
NGAKLPYPVDFLLDEWPNIGAIPDYEETLATCRKYGIGISTIVQSISQVIDKYNKDKANAIIGNHAVILC
LGNVNNDTAKYIKEELGNTTVEFETSSEGSSSGKDSRSKSSNKNVSFTGRALLNEDEISNMELDKAVLIT
KNRNPKIIKKCAYFKMFPDLTEKYLAPQNEYKRKKNASMIERHNKLQKEWDIKQEKLKQKKIEEYKEKER
HQKELEEEQNQVNEVSQNKSEITKSEDEETKTVSDTKKSFYDKLKQKQNANKK

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (3-460)

Protein structure

No available structure.

Host bacterium

• ID: 5188
• GenBank: NZ_CP014448
• Plasmid name: pUSA08-1-SUR24
• Incompatibility group: -
• Plasmid size: 32098 bp
• Coordinate of oriT [Strand]: 20791..20826 [+]
• Host baterium: Staphylococcus aureus strain USA300-SUR24

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA15, AcrIIA21