Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104725
Name   oriT_E9_O|unnamed1 in_silico
Organism   Legionella pneumophila strain E9_O
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP015957 (12412..12444 [+], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..9, 13..19  (ACGTTGC..GCAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_E9_O|unnamed1
GCACGTTGCAAAGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3377 GenBank   WP_019232995
Name   traA_A9E96_RS14990_E9_O|unnamed1 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101374.44 Da        Isoelectric Point: 7.0036

>WP_019232995.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Legionella]
MAIYRFSAQPISRSQGRCVVASSAYRSGEKITDERTGTIYDYTGKNDVVHKEIFLPENAPRWMSERSQLW
NAVELGEHRKDAQLAREVQLALPRELTIEQNTELVREFVQSEFVARGMVADFCFHNPKGDDGLTQPHAHI
LLTLREVNEEGFGLKERAWNDKALLEHWREEWANCQNRHLALHGIDQRVDHRSYKEQGIPLEPQYKIGAK
CAQQHMARLEDHQRIAKENGQLIFERPEIALDALTRNQSTFTHQDLARFINRHTEDAEQFKLVFERVKSC
DELVLLGVDSEGRQRFSTRDMLSIESSMMKQADKLHHQKGHEVDEHALLHAKSNRTLSQEQEVALDYVTS
SGDMKSLLGYAGTGKSYLLGAAREAWEQSGYRVQGAALSGIAALNLSDSSGIESRTIASLFYRLDKGMLT
LNSRDILVVDEAGMLGSRTMERLTREVEKASAKLVLVGDWQQLQAIEAGASFRSIAENYQYAELNQIRRQ
TTPWQVEASFNLAKGDVAHALHAYDIHDHIHRFRTTSEAKHSLIEQWNDVRIAQRCESQIILAYTRKDVK
ELNDMARLIKRRDGELGEDVVFGMERGERAFAVNDRVYFLKREDSLSVINGTLGTIHGIDAKSGVITVAL
DRDDLNKKPQLIQVNTHYYKHMEHGYAATVYKAQGVTVDRTYVLPTKHYDAHSTYVAMTRHRKSCDVFVS
REVFAHDKALVHALNRNRAKDVTLDYTQMASEFARQRGLVSENMPLRSLLKDEHSRKSEPSLSSLMNQVM
GRDVQLEQQKLDAFERQFTKENPSEALHIANSLLSTAAYQAKSLAGLFAPYSKAIARNQLTSVQNEELIR
LIGTLPLDSQVMNAFKKDYPELSTQAELFIKRNEQQLMRIKTIDKELDL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(336-522)

MobA/MobL family (MobA_MobL)

(17-213)


  Protein structure



No available structure.




T4CP

ID   3316 GenBank   WP_019232999
Name   traD_A9E96_RS14970_E9_O|unnamed1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 70946.29 Da        Isoelectric Point: 7.4043

>WP_019232999.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Legionella]
MSKDPNFKHYTRGGQISFHNLRMWDQITKTLSMICLFLWVVFTVVFAWIFIPLEKITHAVAFYYAQFLSV
IGQKHHFELSFHGKTYKQSVDTILHYAYYQDNANHVINSLGKSAFWAFCLAFILGLGLAYYFIKRGKEQS
DSQYVRGSQLKNSNEVKKKILRDKAHSDIRIDQFPLIRDSEVQHLLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIIYDKGCSFIPHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSLDKFLKLILTDEFSALEEYLQGTPAATLVSGKIEKTAISIRATLTTYLKSFSA
LAELNQEGKQPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPDRSRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEIFDLLNTRFFFRSPSSDMARLVAA
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLNCFVRLPGHYPITQLTLDLGFR
TTKTNGFIERSMPTTFNFSQLTSFDDHLESGTNGHMGSEPLTKNKINATLQCIEELNLEPL

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(35-126)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 38585..46768

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E96_RS15105 (A9E96_15105) 33951..34484 + 534 WP_019233022 hypothetical protein -
A9E96_RS15110 (A9E96_15110) 34453..35220 - 768 WP_019233021 VUT family protein -
A9E96_RS15115 (A9E96_15115) 35204..35863 - 660 WP_019233020 S24 family peptidase -
A9E96_RS15120 (A9E96_15120) 36062..36304 + 243 WP_019233019 TraY domain-containing protein -
A9E96_RS15125 (A9E96_15125) 36282..37373 + 1092 WP_019233018 hypothetical protein -
A9E96_RS15130 (A9E96_15130) 37418..38161 + 744 WP_019233017 complement resistance protein TraT -
A9E96_RS15135 (A9E96_15135) 38284..38571 + 288 WP_019233016 hypothetical protein -
A9E96_RS15140 (A9E96_15140) 38585..38881 + 297 WP_019233015 type IV conjugative transfer system protein TraL traL
A9E96_RS15145 (A9E96_15145) 38891..39460 + 570 WP_019233014 type IV conjugative transfer system protein TraE traE
A9E96_RS15150 (A9E96_15150) 39453..40172 + 720 WP_019233013 type-F conjugative transfer system secretin TraK traK
A9E96_RS15155 (A9E96_15155) 40165..41532 + 1368 WP_019233012 TrbI/VirB10 family protein traB
A9E96_RS15160 (A9E96_15160) 41529..41819 + 291 WP_019233011 TraV family lipoprotein traV
A9E96_RS15165 (A9E96_15165) 41810..44398 + 2589 WP_019233010 type IV secretion system protein TraC virb4
A9E96_RS15170 (A9E96_15170) 44395..44733 + 339 WP_223499704 type-F conjugative transfer system protein TrbI -
A9E96_RS15175 (A9E96_15175) 44760..45365 + 606 WP_040522242 type-F conjugative transfer system protein TraW traW
A9E96_RS15180 (A9E96_15180) 45355..46338 + 984 WP_019233007 conjugal transfer pilus assembly protein TraU traU
A9E96_RS15185 (A9E96_15185) 46352..46768 + 417 WP_019233006 type-F conjugative transfer system pilin assembly protein TrbC trbC

Host bacterium

ID   5164 GenBank   NZ_CP015957
Plasmid name   E9_O|unnamed1 Incompatibility group   -
Plasmid size   48354 bp Coordinate of oriT [Strand]   12412..12444 [+]
Host baterium   Legionella pneumophila strain E9_O

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1