Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104724
Name   oriT_C9_S|unnamed1 in_silico
Organism   Legionella pneumophila strain C9_S
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP015942 (19057..19089 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..9, 13..19  (ACGTTGC..GCAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_C9_S|unnamed1
GCACGTTGCAAAGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3376 GenBank   WP_063230552
Name   traA_A9E85_RS15215_C9_S|unnamed1 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101259.36 Da        Isoelectric Point: 6.8584

>WP_063230552.1 Ti-type conjugative transfer relaxase TraA [Legionella pneumophila]
MAIYRFSAQPISRSQGRSVVASSAYRSGEKIIDERTGTTYDYTDKNDVVHKEIFLPESAPQWMSERSKLW
NAVELGENRKDAQLAREVQLALPRELTIEQNTELVREFVKTEFVALGMVADVCFHNPKGDDGLYQPHAHI
LLTLREVDEEGFGLKERAWNDKALLEHWREEWANCQNRHLALHGIDQRVDHRSYKEQGIPLEPQYKIGAK
CAQQHMARLADHQRIAKENGQLIFEKPEIALDALTRNQSTFTHQDLARFINRHTEDAEQFKLVFERVKTS
TELVFVGLDSEGKQRFSTHDMLSIESAMMKLADKLYHQKGHQVDEHAVRNAKSNRTLSQEQETALDYVTS
IGDMKSLLGYAGTGKSYLLGAAREAWEQSGYRVQGTALSGIAALNLSDSSGIESRTIASLFYRLDKGMLT
LNSRDILVVDEAGMLGSRTMERLTREVEKASAKLVLVGDWQQLQAIEAGAAFRSIAENYQYAELNQIRRQ
TTPWQVEASLHLAQGQVEHALQAYDLHDHIHRFKTTSEAKQFLIEQWNDVRIAQPSESQIILAYTRKDVK
ELNDMARAIKRRDGELGEDIIFTMERGERAFAVNDRVYFLKREDSLSVINGTLGTIQGIDAKSGVITVAL
DRDDLNKKPQLVQVNTHYYKHMEHGYAATVYKAQGVTVDRAYVLPTKHYDAHSTYVAMTRHRKSCDVFVS
REVFAHDKALVHALNRNRAKDVTLDYTQMAAEFARQRGLICESKTLPSLSKDEYFRKSEPSLSSLMNQVM
GRDVQLEQQRLDAFERQFTKENPSEALHIANSLLSTAAYQAKSLAGLFAPYSKAIARNQLTSVQNEELIR
LIGTLPLDSQVMNAFKKDYPELSTQAEQFIKRNEQQLMRIKTIDKELDL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(336-523)

MobA/MobL family (MobA_MobL)

(17-213)


  Protein structure



No available structure.




T4CP

ID   3315 GenBank   WP_080452554
Name   traD_A9E85_RS15225_C9_S|unnamed1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 70871.29 Da        Isoelectric Point: 7.4044

>WP_080452554.1 type IV conjugative transfer system coupling protein TraD [Legionella pneumophila]
MSKDPNFKHYTRGGQISFHNLRMWDQITKTLSMICLFLWVVFTVVFAWIFIPLEKIAHAIAFYYAQFLSL
VGQKHNFELSFHGKAYKQTVDTILHYSYYQDNATHVVNSLGKSAFWAFCLSFILGLGLAYYFIKRGKAQS
DSQFVRGSQLKNSNAVKKKILRDKAHSDIMIDQFPLIRDSEVQHLLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIVYDKGCSFIPHYYHDDSDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSLDKFLKLILTDEFSELEEYLQGTPAATLVSGKIEKTAISIRATLTTYLKSFSA
LAELNQEGKQPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPDRNRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEIFDLLNTRFFFRSPSSDMARLVAA
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLHCFVRLPGHYPITQLALDLGFR
TTKTNGFIERSMPTTFNFSQLTSFDDNLESENNGHMGNDPVIKNKITAKLQCIDELNLEPL

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(35-126)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 343..7126

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E85_RS15095 (A9E85_15095) 343..972 - 630 WP_227003449 type-F conjugative transfer system protein TraW traW
A9E85_RS15100 (A9E85_15100) 975..1313 - 339 WP_227003448 type-F conjugative transfer system protein TrbI -
A9E85_RS15105 (A9E85_15105) 1310..3898 - 2589 WP_063230486 type IV secretion system protein TraC virb4
A9E85_RS15110 (A9E85_15110) 3889..4179 - 291 WP_063230488 TraV family lipoprotein traV
A9E85_RS15115 (A9E85_15115) 4176..5549 - 1374 WP_063230491 TrbI/VirB10 family protein traB
A9E85_RS15120 (A9E85_15120) 5542..6258 - 717 WP_063230494 type-F conjugative transfer system secretin TraK traK
A9E85_RS15125 (A9E85_15125) 6251..6820 - 570 WP_063230496 type IV conjugative transfer system protein TraE traE
A9E85_RS15130 (A9E85_15130) 6830..7126 - 297 WP_063230499 type IV conjugative transfer system protein TraL traL
A9E85_RS15135 (A9E85_15135) 7140..7427 - 288 WP_063230501 hypothetical protein -
A9E85_RS15140 (A9E85_15140) 7549..8292 - 744 WP_063230504 complement resistance protein TraT -
A9E85_RS15145 (A9E85_15145) 8395..9009 - 615 WP_063230506 complement resistance protein TraT -
A9E85_RS15150 (A9E85_15150) 9028..9234 - 207 WP_063230508 TraY domain-containing protein -
A9E85_RS15155 (A9E85_15155) 9471..10220 + 750 WP_063230511 S24 family peptidase -
A9E85_RS15160 (A9E85_15160) 10269..10967 + 699 WP_063230513 class I SAM-dependent methyltransferase -
A9E85_RS15165 (A9E85_15165) 10973..11902 - 930 WP_063230515 queuosine precursor transporter -

Region 2: 22540..32714

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E85_RS16050 18115..18351 - 237 WP_080477597 hypothetical protein -
A9E85_RS15205 (A9E85_15205) 18363..18623 - 261 WP_145939442 hypothetical protein -
A9E85_RS15210 (A9E85_15210) 18636..18932 - 297 WP_063230549 hypothetical protein -
A9E85_RS15215 (A9E85_15215) 19158..21827 + 2670 WP_063230552 Ti-type conjugative transfer relaxase TraA -
A9E85_RS15220 (A9E85_15220) 21838..22455 + 618 WP_063230554 hypothetical protein -
A9E85_RS15225 (A9E85_15225) 22540..24405 - 1866 WP_080452554 type IV conjugative transfer system coupling protein TraD virb4
A9E85_RS15230 (A9E85_15230) 24405..25163 - 759 WP_145939443 hypothetical protein -
A9E85_RS15235 (A9E85_15235) 25160..27991 - 2832 WP_063230558 conjugal transfer mating-pair stabilization protein TraG traG
A9E85_RS15240 (A9E85_15240) 28004..29368 - 1365 WP_063230560 conjugal transfer protein TraH traH
A9E85_RS15245 (A9E85_15245) 29365..29874 - 510 WP_063230563 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
A9E85_RS15250 (A9E85_15250) 29874..30617 - 744 WP_063230565 type-F conjugative transfer system pilin assembly protein TraF traF
A9E85_RS15255 (A9E85_15255) 30610..32301 - 1692 WP_070071256 type-F conjugative transfer system mating-pair stabilization protein TraN traN
A9E85_RS15260 (A9E85_15260) 32298..32714 - 417 WP_063230477 type-F conjugative transfer system pilin assembly protein TrbC trbC

Host bacterium

ID   5163 GenBank   NZ_CP015942
Plasmid name   C9_S|unnamed1 Incompatibility group   -
Plasmid size   33359 bp Coordinate of oriT [Strand]   19057..19089 [-]
Host baterium   Legionella pneumophila strain C9_S

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -