Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104719 |
| Name | oriT_X-CRHVKP-130|p3  |
| Organism | Klebsiella pneumoniae strain X-CRHVKP-130 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP119177 (79869..79950 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_X-CRHVKP-130|p3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3373 | GenBank | WP_032440569 |
| Name | Relaxase_PY995_RS27100_X-CRHVKP-130|p3 |
UniProt ID | A0A7G3L4G1 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74384.24 Da Isoelectric Point: 8.3203
>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L4G1 |
T4CP
| ID | 3310 | GenBank | WP_032440593 |
| Name | trbC_PY995_RS26755_X-CRHVKP-130|p3 |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82679.78 Da Isoelectric Point: 6.3201
>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1964..23897
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PY995_RS26645 (PY995_26645) | 39..266 | + | 228 | WP_032440573 | hypothetical protein | - |
| PY995_RS26650 (PY995_26650) | 998..1477 | + | 480 | WP_032440574 | lytic transglycosylase domain-containing protein | - |
| PY995_RS26655 (PY995_26655) | 1503..1967 | + | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| PY995_RS26660 (PY995_26660) | 1964..2782 | + | 819 | WP_032440576 | type IV secretory system conjugative DNA transfer family protein | traI |
| PY995_RS26665 (PY995_26665) | 2760..3947 | + | 1188 | WP_077255878 | plasmid transfer ATPase TraJ | - |
| PY995_RS26670 (PY995_26670) | 3944..4210 | + | 267 | WP_032440577 | IcmT/TraK family protein | traK |
| PY995_RS26675 (PY995_26675) | 4228..7641 | + | 3414 | WP_052145516 | LPD7 domain-containing protein | - |
| PY995_RS26680 (PY995_26680) | 7675..8028 | + | 354 | WP_032440584 | hypothetical protein | traL |
| PY995_RS26685 (PY995_26685) | 8100..8768 | + | 669 | WP_032440585 | DotI/IcmL/TraM family protein | traM |
| PY995_RS26690 (PY995_26690) | 8779..9882 | + | 1104 | WP_077264204 | DotH/IcmK family type IV secretion protein | traN |
| PY995_RS26695 (PY995_26695) | 9886..11226 | + | 1341 | WP_032440586 | conjugal transfer protein TraO | traO |
| PY995_RS26700 (PY995_26700) | 11233..11952 | + | 720 | WP_050597333 | conjugal transfer protein TraP | traP |
| PY995_RS26705 (PY995_26705) | 11962..12495 | + | 534 | WP_032440587 | conjugal transfer protein TraQ | traQ |
| PY995_RS26710 (PY995_26710) | 12551..12952 | + | 402 | WP_032440588 | DUF6750 family protein | traR |
| PY995_RS26715 (PY995_26715) | 12996..13559 | + | 564 | WP_045325070 | hypothetical protein | traT |
| PY995_RS26720 (PY995_26720) | 13569..16616 | + | 3048 | WP_032440589 | conjugal transfer protein | traU |
| PY995_RS26725 (PY995_26725) | 16626..17822 | + | 1197 | WP_032440590 | conjugal transfer protein TraW | traW |
| PY995_RS26730 (PY995_26730) | 17819..18400 | + | 582 | WP_050597335 | conjugal transfer protein TraX | - |
| PY995_RS26735 (PY995_26735) | 18442..20586 | + | 2145 | WP_032440591 | DotA/TraY family protein | traY |
| PY995_RS26740 (PY995_26740) | 20652..21275 | + | 624 | WP_032440592 | plasmid IncI1-type surface exclusion protein ExcA | - |
| PY995_RS26745 (PY995_26745) | 21377..22795 | + | 1419 | WP_063666705 | hypothetical protein | trbA |
| PY995_RS26750 (PY995_26750) | 22806..23897 | + | 1092 | WP_050597336 | thioredoxin fold domain-containing protein | trbB |
| PY995_RS26755 (PY995_26755) | 23899..26091 | + | 2193 | WP_032440593 | F-type conjugative transfer protein TrbC | - |
| PY995_RS26760 (PY995_26760) | 26137..26670 | + | 534 | WP_032440594 | phospholipase D family protein | - |
| PY995_RS26765 (PY995_26765) | 26682..26933 | + | 252 | WP_113394424 | hypothetical protein | - |
| PY995_RS26770 (PY995_26770) | 27439..28134 | + | 696 | WP_254910806 | RepA family replication protein | - |
Host bacterium
| ID | 5158 | GenBank | NZ_CP119177 |
| Plasmid name | X-CRHVKP-130|p3 | Incompatibility group | IncFII |
| Plasmid size | 87095 bp | Coordinate of oriT [Strand] | 79869..79950 [+] |
| Host baterium | Klebsiella pneumoniae strain X-CRHVKP-130 |
Cargo genes
| Drug resistance gene | sul2, dfrA14, catA2, tet(A), blaLAP-2, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |