Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104675 |
| Name | oriT_pLSW74_2  |
| Organism | Latilactobacillus sakei strain WiKim0074 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP025208 (48..85 [-], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | 1..7, 19..25 (ACACAAC..GTTGTGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_pLSW74_2
ACACAACCAACTTTAGTTGTTGTGTGTAAGTGCGCATT
ACACAACCAACTTTAGTTGTTGTGTGTAAGTGCGCATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3345 | GenBank | WP_112234124 |
| Name | mobQ_CXB69_RS09960_pLSW74_2 |
UniProt ID | _ |
| Length | 687 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 687 a.a. Molecular weight: 80356.78 Da Isoelectric Point: 9.3458
>WP_112234124.1 MobQ family relaxase [Latilactobacillus sakei]
MAIFHMSFQNISAGKGRSAIAGASYRSGEKLFDQKEGRSYFYARSVMPESFILTPKNAPEWASDREKLWN
EVERKDRRSNSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRENILDENGNKTYTGNSRFPRSRKVWLVDWDKKEKITEWRHNWAASVNQVLEQKNIP
DRISEKSFIEQGIDDTPTQHEGINSKRHERKEFNQQVKDYRKAKASYKNNQEKVINRGHLDSLSKHFSFN
EKRVVKELSHELKTYISLENLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DHTTKKLYPELNFEQTTQAERRELIKETESEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGFKLL
MQQEKEAKIDLKYTLMIHSDSLESLEHVDKGLLEKYSPAEQQKITRAVKDLRAIMAVKQVIKTQYHEVLK
SAFPKGDLDGLPLIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDRL
ENHHLQRVLKHDGTKQLFLGECKVDPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGYYQPLNYKPVSPDY
YLKTAFSNAIMTALYARDEDYQRQKQAQGLKETEWEMTKKQRQHQTRNRHEDGGMHL
MAIFHMSFQNISAGKGRSAIAGASYRSGEKLFDQKEGRSYFYARSVMPESFILTPKNAPEWASDREKLWN
EVERKDRRSNSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRENILDENGNKTYTGNSRFPRSRKVWLVDWDKKEKITEWRHNWAASVNQVLEQKNIP
DRISEKSFIEQGIDDTPTQHEGINSKRHERKEFNQQVKDYRKAKASYKNNQEKVINRGHLDSLSKHFSFN
EKRVVKELSHELKTYISLENLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DHTTKKLYPELNFEQTTQAERRELIKETESEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGFKLL
MQQEKEAKIDLKYTLMIHSDSLESLEHVDKGLLEKYSPAEQQKITRAVKDLRAIMAVKQVIKTQYHEVLK
SAFPKGDLDGLPLIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDRL
ENHHLQRVLKHDGTKQLFLGECKVDPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGYYQPLNYKPVSPDY
YLKTAFSNAIMTALYARDEDYQRQKQAQGLKETEWEMTKKQRQHQTRNRHEDGGMHL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3285 | GenBank | WP_112234134 |
| Name | t4cp2_CXB69_RS10020_pLSW74_2 |
UniProt ID | _ |
| Length | 517 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 517 a.a. Molecular weight: 58110.33 Da Isoelectric Point: 4.8806
>WP_112234134.1 VirD4-like conjugal transfer protein, CD1115 family [Latilactobacillus sakei]
MNGTILGIVDKRIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPIDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRAPDQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLDLIMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGNDKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNDYQGQPEASQETNFKNKVEKWKAKLKETAQKKAANKMSQTEEEQQQDSL
DSDLERVSNKDSQTEEPEEDDDNNLLG
MNGTILGIVDKRIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGIK
LAQGYQVHVVNFANMAHSDRYNPIDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRAPDQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLDLIMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGNDKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNDYQGQPEASQETNFKNKVEKWKAKLKETAQKKAANKMSQTEEEQQQDSL
DSDLERVSNKDSQTEEPEEDDDNNLLG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3632..13482
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CXB69_RS09960 (CXB69_09945) | 170..2233 | + | 2064 | WP_112234124 | MobQ family relaxase | - |
| CXB69_RS10365 (CXB69_09950) | 2318..2629 | + | 312 | WP_112234125 | hypothetical protein | - |
| CXB69_RS09970 (CXB69_09955) | 2660..3274 | + | 615 | WP_112234126 | hypothetical protein | - |
| CXB69_RS09975 (CXB69_09960) | 3276..3611 | + | 336 | WP_061827386 | CagC family type IV secretion system protein | - |
| CXB69_RS09980 (CXB69_09965) | 3632..3994 | + | 363 | WP_014216295 | hypothetical protein | trsC |
| CXB69_RS09985 (CXB69_09970) | 3963..4622 | + | 660 | WP_112234127 | TrsD/TraD family conjugative transfer protein | trsD |
| CXB69_RS09990 (CXB69_09975) | 4634..6649 | + | 2016 | WP_112234128 | DUF87 domain-containing protein | virb4 |
| CXB69_RS09995 (CXB69_09980) | 6642..8060 | + | 1419 | WP_112234129 | type VII secretion protein EssB/YukC | trsF |
| CXB69_RS10000 (CXB69_09985) | 8061..9215 | + | 1155 | WP_112234130 | phage tail tip lysozyme | orf14 |
| CXB69_RS10005 (CXB69_09990) | 9230..9847 | + | 618 | WP_112234131 | hypothetical protein | - |
| CXB69_RS10010 (CXB69_09995) | 9834..10202 | + | 369 | WP_112234132 | thioredoxin family protein | - |
| CXB69_RS10015 (CXB69_10000) | 10205..10675 | + | 471 | WP_112234133 | conjugal transfer protein | trsJ |
| CXB69_RS10020 (CXB69_10005) | 10677..12230 | + | 1554 | WP_112234134 | VirD4-like conjugal transfer protein, CD1115 family | - |
| CXB69_RS10025 (CXB69_10010) | 12245..12619 | + | 375 | WP_112234135 | hypothetical protein | - |
| CXB69_RS10030 (CXB69_10015) | 12643..13482 | + | 840 | WP_061827391 | conjugal transfer protein TrbL family protein | trsL |
| CXB69_RS10035 (CXB69_10020) | 13498..13908 | + | 411 | WP_112234136 | hypothetical protein | - |
| CXB69_RS10040 (CXB69_10025) | 13915..16050 | + | 2136 | WP_112234137 | type IA DNA topoisomerase | - |
| CXB69_RS10045 (CXB69_10030) | 16173..16388 | + | 216 | WP_034528478 | hypothetical protein | - |
| CXB69_RS10050 (CXB69_10035) | 16392..17516 | + | 1125 | WP_112234138 | ArdC-like ssDNA-binding domain-containing protein | - |
| CXB69_RS10370 | 17672..17761 | - | 90 | WP_072672334 | putative holin-like toxin | - |
| CXB69_RS10375 (CXB69_10045) | 17947..18087 | - | 141 | Protein_20 | restriction endonuclease subunit S | - |
Host bacterium
| ID | 5114 | GenBank | NZ_CP025208 |
| Plasmid name | pLSW74_2 | Incompatibility group | - |
| Plasmid size | 44839 bp | Coordinate of oriT [Strand] | 48..85 [-] |
| Host baterium | Latilactobacillus sakei strain WiKim0074 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |