Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104652
Name   oriT_pSMO-2010K-0257 in_silico
Organism   Salmonella enterica subsp. enterica serovar Montevideo str. CDC 2010K-0257
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP020913 (48667..48817 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pSMO-2010K-0257
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3337 GenBank   WP_001326173
Name   mobH_AW77_RS24300_pSMO-2010K-0257 insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   3277 GenBank   WP_000178857
Name   traD_AW77_RS24305_pSMO-2010K-0257 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   3278 GenBank   WP_000637384
Name   traC_AW77_RS24380_pSMO-2010K-0257 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46751..71894

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW77_RS24290 (AW77_24045) 42782..43015 - 234 WP_001191890 hypothetical protein -
AW77_RS24295 (AW77_24050) 42997..43614 - 618 WP_001249395 hypothetical protein -
AW77_RS24300 (AW77_24055) 43782..46754 + 2973 WP_001326173 MobH family relaxase -
AW77_RS24305 (AW77_24060) 46751..48616 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
AW77_RS24310 (AW77_24065) 48666..49211 + 546 WP_000228720 hypothetical protein -
AW77_RS24315 (AW77_24070) 49168..49797 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
AW77_RS24320 (AW77_24075) 49807..50253 + 447 WP_000122507 hypothetical protein -
AW77_RS24325 (AW77_24080) 50263..50640 + 378 WP_000869297 hypothetical protein -
AW77_RS24330 (AW77_24085) 50640..51302 + 663 WP_001231464 hypothetical protein -
AW77_RS24335 (AW77_24090) 51638..52003 + 366 WP_001052530 hypothetical protein -
AW77_RS24340 (AW77_24095) 52148..52429 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AW77_RS24345 (AW77_24100) 52426..53052 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
AW77_RS24350 (AW77_24105) 53036..53953 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AW77_RS24355 (AW77_24110) 53953..55266 + 1314 WP_024131605 TraB/VirB10 family protein traB
AW77_RS24360 (AW77_24115) 55263..55841 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AW77_RS24365 (AW77_24120) 55845..56237 + 393 WP_000479535 TraA family conjugative transfer protein -
AW77_RS24370 (AW77_24125) 56438..61924 + 5487 WP_000606835 Ig-like domain-containing protein -
AW77_RS24375 (AW77_24130) 62073..62780 + 708 WP_001259346 DsbC family protein -
AW77_RS24380 (AW77_24135) 62777..65224 + 2448 WP_000637384 type IV secretion system protein TraC virb4
AW77_RS24385 (AW77_24140) 65239..65556 + 318 WP_000351984 hypothetical protein -
AW77_RS24390 (AW77_24145) 65553..66083 + 531 WP_001010740 S26 family signal peptidase -
AW77_RS24395 (AW77_24150) 66046..67311 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
AW77_RS24400 (AW77_24155) 67308..67979 + 672 WP_000575345 EAL domain-containing protein -
AW77_RS24405 (AW77_24160) 67979..68983 + 1005 WP_005507569 TraU family protein traU
AW77_RS24410 (AW77_24165) 69099..71894 + 2796 WP_001256484 conjugal transfer mating pair stabilization protein TraN traN
AW77_RS24415 (AW77_24170) 71933..72793 - 861 WP_000709517 hypothetical protein -
AW77_RS24420 (AW77_24175) 72916..73557 - 642 WP_000796664 hypothetical protein -
AW77_RS24425 (AW77_24180) 73852..74172 + 321 WP_000547566 hypothetical protein -
AW77_RS24430 (AW77_24185) 74476..74661 + 186 WP_001186917 hypothetical protein -
AW77_RS24435 (AW77_24190) 74881..75849 + 969 WP_000085160 AAA family ATPase -
AW77_RS24440 (AW77_24195) 75860..76768 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   5091 GenBank   NZ_CP020913
Plasmid name   pSMO-2010K-0257 Incompatibility group   IncA/C2
Plasmid size   119867 bp Coordinate of oriT [Strand]   48667..48817 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Montevideo str. CDC 2010K-0257

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merR, merT
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -