Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104642
Name   oriT_FDAARGOS_1271|unnamed in_silico
Organism   Salmonella enterica strain FDAARGOS_1271
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP069519 (84600..84750 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      12..17, 24..29  (AACCCG..CGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_FDAARGOS_1271|unnamed
TGGTCGATGTGAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATATCCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTGTTACATCACAGATGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3333 GenBank   WP_000909947
Name   mobH_I6K01_RS23115_FDAARGOS_1271|unnamed insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109404.96 Da        Isoelectric Point: 4.8922

>WP_000909947.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGSEQKTKDKSLQQQPKEKQGNVASLVEEQKRKPVQDQ
EKQNVARLPKREAQPVAPKPKVEHERELGHVEVRERDEPEVREFEPPKAKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   3273 GenBank   WP_000178858
Name   traD_I6K01_RS23120_FDAARGOS_1271|unnamed insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69248.76 Da        Isoelectric Point: 7.1093

>WP_000178858.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWMAASCAALAVEQVSTMPSEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVLTHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVKPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNTDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   3274 GenBank   WP_000587426
Name   traC_I6K01_RS23200_FDAARGOS_1271|unnamed insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92489.76 Da        Isoelectric Point: 5.9204

>WP_000587426.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIITIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDQSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTSLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRILAKTSKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQHLAQIRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYDDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRKQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 82684..107788

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K01_RS23105 (I6K01_23105) 78724..78957 - 234 WP_001191893 hypothetical protein -
I6K01_RS23110 (I6K01_23110) 78939..79556 - 618 WP_079778879 hypothetical protein -
I6K01_RS23115 (I6K01_23115) 79724..82687 + 2964 WP_000909947 MobH family relaxase -
I6K01_RS23120 (I6K01_23120) 82684..84549 + 1866 WP_000178858 conjugative transfer system coupling protein TraD virb4
I6K01_RS23125 (I6K01_23125) 84599..85144 + 546 WP_023356350 hypothetical protein -
I6K01_RS23130 (I6K01_23130) 85101..85730 + 630 WP_000743448 DUF4400 domain-containing protein tfc7
I6K01_RS23135 (I6K01_23135) 85741..86187 + 447 WP_000122583 hypothetical protein -
I6K01_RS23140 (I6K01_23140) 86197..86574 + 378 WP_000869260 hypothetical protein -
I6K01_RS23145 (I6K01_23145) 86574..87236 + 663 WP_000045163 hypothetical protein -
I6K01_RS23150 (I6K01_23150) 87415..87558 + 144 WP_023356351 hypothetical protein -
I6K01_RS23155 (I6K01_23155) 87571..87918 + 348 WP_001102160 hypothetical protein -
I6K01_RS23160 (I6K01_23160) 88061..88342 + 282 WP_000805626 type IV conjugative transfer system protein TraL traL
I6K01_RS23165 (I6K01_23165) 88339..88965 + 627 WP_001049718 TraE/TraK family type IV conjugative transfer system protein traE
I6K01_RS23170 (I6K01_23170) 88949..89866 + 918 WP_000839951 type-F conjugative transfer system secretin TraK traK
I6K01_RS23175 (I6K01_23175) 89863..91179 + 1317 WP_000595204 TraB/VirB10 family protein traB
I6K01_RS23180 (I6K01_23180) 91176..91754 + 579 WP_000794193 type IV conjugative transfer system lipoprotein TraV traV
I6K01_RS23185 (I6K01_23185) 91758..92150 + 393 WP_012766327 TraA family conjugative transfer protein -
I6K01_RS23190 (I6K01_23190) 92351..97837 + 5487 WP_000606836 Ig-like domain-containing protein -
I6K01_RS23195 (I6K01_23195) 97986..98693 + 708 WP_001259344 DsbC family protein -
I6K01_RS23200 (I6K01_23200) 98690..101137 + 2448 WP_000587426 type IV secretion system protein TraC virb4
I6K01_RS23205 (I6K01_23205) 101152..101469 + 318 WP_000364398 hypothetical protein -
I6K01_RS23210 (I6K01_23210) 101466..101996 + 531 WP_000672456 S26 family signal peptidase -
I6K01_RS23215 (I6K01_23215) 101959..103227 + 1269 WP_001553264 TrbC family F-type conjugative pilus assembly protein traW
I6K01_RS23220 (I6K01_23220) 103224..103901 + 678 WP_000577801 EAL domain-containing protein -
I6K01_RS23225 (I6K01_23225) 103895..104899 + 1005 WP_000911304 TraU family protein traU
I6K01_RS23230 (I6K01_23230) 105050..107788 + 2739 WP_001256493 conjugal transfer mating pair stabilization protein TraN traN
I6K01_RS23235 (I6K01_23235) 107827..108687 - 861 WP_000679674 hypothetical protein -
I6K01_RS23240 (I6K01_23240) 108799..109449 - 651 WP_000796641 hypothetical protein -
I6K01_RS23245 (I6K01_23245) 109740..110060 + 321 WP_000547567 hypothetical protein -
I6K01_RS23250 (I6K01_23250) 110371..110556 + 186 WP_001186892 hypothetical protein -
I6K01_RS23255 (I6K01_23255) 110777..111745 + 969 WP_001553257 AAA family ATPase -
I6K01_RS23260 (I6K01_23260) 111757..112662 + 906 WP_000739141 hypothetical protein -

Host bacterium

ID   5081 GenBank   NZ_CP069519
Plasmid name   FDAARGOS_1271|unnamed Incompatibility group   IncA/C
Plasmid size   128163 bp Coordinate of oriT [Strand]   84600..84750 [+]
Host baterium   Salmonella enterica strain FDAARGOS_1271

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -