Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104615
Name   oriT_pBY369-1 in_silico
Organism   Vibrio cholerae strain BY369
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP090380 (7155..7503 [-], 349 nt)
oriT length   349 nt
IRs (inverted repeats)      134..140, 154..160  (AAAATAT..ATATTTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 349 nt

>oriT_pBY369-1
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGGTGACCTACAGTCGGTTCTTGTTGGAGAATTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3317 GenBank   WP_000539530
Name   Relaxase_LVJ30_RS19010_pBY369-1 insolico UniProt ID   A0A611DMI3
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44497.84 Da        Isoelectric Point: 10.5286

>WP_000539530.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFFNI

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(70-228)


  Protein structure


Source ID Structure
AlphaFold DB A0A611DMI3


Host bacterium

ID   5054 GenBank   NZ_CP090380
Plasmid name   pBY369-1 Incompatibility group   IncX1
Plasmid size   41043 bp Coordinate of oriT [Strand]   7155..7503 [-]
Host baterium   Vibrio cholerae strain BY369

Cargo genes

Drug resistance gene   blaTEM-1B, aph(6)-Id, aph(3'')-Ib, tet(B)
Virulence gene   mrkA, mrkB, mrkC, mrkD, mrkF
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -