Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 104611 |
Name | oriT_pM013 |
Organism | Staphylococcus aureus subsp. aureus M013 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP039996 (1515..1754 [+], 240 nt) |
oriT length | 240 nt |
IRs (inverted repeats) | 217..222, 232..237 (ATTTTA..TAAAAT) 171..178, 183..190 (CTATCATT..AATGATAG) 154..160, 164..170 (GTCTGGC..GCCAGAC) 26..32, 44..50 (TTTTTTA..TAAAAAA) 37..42, 45..50 (TTTTTT..AAAAAA) 1..7, 20..26 (AAGACAT..ATGTCTT) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 240 nt
>oriT_pM013
AAGACATTAGTGATAACTGATGTCTTTTTTTATTGATTTTTTGTAAAAAAGTACTGTCTTAGTTTTGTGACAAATACTGTATATAGTGTCACAAAAGTGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTTTTGAATGATAGCAAATTCCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTC
AAGACATTAGTGATAACTGATGTCTTTTTTTATTGATTTTTTGTAAAAAAGTACTGTCTTAGTTTTGTGACAAATACTGTATATAGTGTCACAAAAGTGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTTTTGAATGATAGCAAATTCCCCTTATGCTCTTACGGAGATTTTAGAGTTAAATTAAAATTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 3314 | GenBank | WP_000122374 |
Name | mobV_M013TW_RS14080_pM013 | UniProt ID | A0A2R4A9A7 |
Length | 413 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 413 a.a. Molecular weight: 48526.00 Da Isoelectric Point: 6.1679
>WP_000122374.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGK
RKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVP
ITDDGRLSAKEVVGNKKALTAFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERE
SQKTDHIKQKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKA
AQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIEIALKLLKILL
KELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQEQQKNQKRDRGMHL
MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGK
RKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVP
ITDDGRLSAKEVVGNKKALTAFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERE
SQKTDHIKQKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKA
AQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIEIALKLLKILL
KELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQEQQKNQKRDRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2R4A9A7 |
Host bacterium
ID | 5050 | GenBank | NZ_CP039996 |
Plasmid name | pM013 | Incompatibility group | - |
Plasmid size | 21682 bp | Coordinate of oriT [Strand] | 1515..1754 [+] |
Host baterium | Staphylococcus aureus subsp. aureus M013 |
Cargo genes
Drug resistance gene | tet(K) |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrIIA21 |