Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104604 |
| Name | oriT_FO15|unnamed3  |
| Organism | Klebsiella pneumoniae strain FO15 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP073005 (19612..19664 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_FO15|unnamed3
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3254 | GenBank | WP_000338974 |
| Name | t4cp2_KBT91_RS28255_FO15|unnamed3 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73347.89 Da Isoelectric Point: 9.1391
>WP_000338974.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 42439..60138
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBT91_RS28125 | 38409..38915 | + | 507 | WP_001358485 | CaiF/GrlA family transcriptional regulator | - |
| KBT91_RS28130 | 38908..39102 | + | 195 | WP_000049865 | DUF1187 family protein | - |
| KBT91_RS28135 | 39148..40101 | + | 954 | WP_072105959 | SPFH domain-containing protein | - |
| KBT91_RS28140 | 40174..40617 | + | 444 | WP_000964840 | NfeD family protein | - |
| KBT91_RS28145 | 40621..40791 | + | 171 | WP_000550721 | hypothetical protein | - |
| KBT91_RS28150 | 40802..41464 | + | 663 | WP_001419740 | hypothetical protein | - |
| KBT91_RS28155 | 41648..41863 | + | 216 | WP_001360344 | hypothetical protein | - |
| KBT91_RS28160 | 41879..42181 | + | 303 | WP_001360345 | hypothetical protein | - |
| KBT91_RS28165 | 42178..42435 | + | 258 | WP_000739144 | hypothetical protein | - |
| KBT91_RS28170 | 42439..43425 | + | 987 | WP_001419739 | type IV secretion system protein | virB6 |
| KBT91_RS28175 | 43431..44078 | + | 648 | WP_001419738 | type IV secretion system protein | - |
| KBT91_RS28180 | 44082..44318 | + | 237 | WP_000750964 | EexN family lipoprotein | - |
| KBT91_RS28185 | 44365..45174 | - | 810 | WP_001419737 | DUF5710 domain-containing protein | - |
| KBT91_RS28190 | 45222..45854 | + | 633 | WP_001419736 | hypothetical protein | - |
| KBT91_RS28195 | 45921..46220 | + | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| KBT91_RS28200 | 46223..47458 | + | 1236 | WP_001419735 | TcpQ domain-containing protein | - |
| KBT91_RS28205 | 47464..47901 | + | 438 | WP_015387358 | type IV pilus biogenesis protein PilM | - |
| KBT91_RS28210 | 48241..48639 | + | 399 | WP_001708012 | hypothetical protein | - |
| KBT91_RS28215 | 48660..49244 | + | 585 | WP_001401693 | lytic transglycosylase domain-containing protein | virB1 |
| KBT91_RS28220 | 49244..49534 | + | 291 | WP_000865479 | conjugal transfer protein | - |
| KBT91_RS28225 | 49605..49925 | + | 321 | WP_000362081 | VirB3 family type IV secretion system protein | virB3 |
| KBT91_RS28230 | 49931..52288 | + | 2358 | WP_015387356 | VirB4 family type IV secretion system protein | virb4 |
| KBT91_RS28235 | 52452..53186 | + | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| KBT91_RS28240 | 53252..53953 | + | 702 | WP_053882504 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| KBT91_RS28245 | 53943..55082 | + | 1140 | WP_015387354 | TrbI/VirB10 family protein | virB10 |
| KBT91_RS28250 | 55101..56156 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| KBT91_RS28255 | 56172..58130 | + | 1959 | WP_000338974 | type IV secretory system conjugative DNA transfer family protein | - |
| KBT91_RS28260 | 58177..60138 | + | 1962 | WP_241420257 | PilN family type IVB pilus formation outer membrane protein | virb4 |
| KBT91_RS28265 | 60189..61499 | + | 1311 | WP_001420476 | type 4b pilus protein PilO2 | - |
| KBT91_RS28270 | 61483..61977 | + | 495 | WP_000912551 | type IV pilus biogenesis protein PilP | - |
Host bacterium
| ID | 5043 | GenBank | NZ_CP073005 |
| Plasmid name | FO15|unnamed3 | Incompatibility group | IncI2 |
| Plasmid size | 62001 bp | Coordinate of oriT [Strand] | 19612..19664 [+] |
| Host baterium | Klebsiella pneumoniae strain FO15 |
Cargo genes
| Drug resistance gene | blaCTX-M-55 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |