Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104599
Name   oriT_SWHEFF_72|unnamed2 in_silico
Organism   Klebsiella quasipneumoniae strain SWHEFF_72
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP055012 (45453..45603 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      12..17, 24..29  (AACCCG..CGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_SWHEFF_72|unnamed2
TGGTCGATGTGAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATATCCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTGTTACATCACAGATGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3308 GenBank   WP_000909947
Name   mobH_HUZ79_RS27925_SWHEFF_72|unnamed2 insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109404.96 Da        Isoelectric Point: 4.8922

>WP_000909947.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGSEQKTKDKSLQQQPKEKQGNVASLVEEQKRKPVQDQ
EKQNVARLPKREAQPVAPKPKVEHERELGHVEVRERDEPEVREFEPPKAKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   3250 GenBank   WP_000178858
Name   traD_HUZ79_RS27930_SWHEFF_72|unnamed2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69248.76 Da        Isoelectric Point: 7.1093

>WP_000178858.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWMAASCAALAVEQVSTMPSEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVLTHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVKPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNTDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   3251 GenBank   WP_000587426
Name   traC_HUZ79_RS28015_SWHEFF_72|unnamed2 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92489.76 Da        Isoelectric Point: 5.9204

>WP_000587426.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIITIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDQSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTSLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRILAKTSKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQHLAQIRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYDDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRKQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 43537..69953

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ79_RS27915 (HUZ79_27855) 39577..39810 - 234 WP_001191893 hypothetical protein -
HUZ79_RS27920 (HUZ79_27860) 39792..40409 - 618 WP_079778879 hypothetical protein -
HUZ79_RS27925 (HUZ79_27865) 40577..43540 + 2964 WP_000909947 MobH family relaxase -
HUZ79_RS27930 (HUZ79_27870) 43537..45402 + 1866 WP_000178858 conjugative transfer system coupling protein TraD virb4
HUZ79_RS27935 (HUZ79_27875) 45452..45997 + 546 WP_023356350 hypothetical protein -
HUZ79_RS27940 (HUZ79_27880) 45954..46583 + 630 WP_000743448 DUF4400 domain-containing protein tfc7
HUZ79_RS27945 (HUZ79_27885) 46594..47040 + 447 WP_000122583 hypothetical protein -
HUZ79_RS27950 (HUZ79_27890) 47050..47427 + 378 WP_000869260 hypothetical protein -
HUZ79_RS27955 (HUZ79_27895) 47427..48089 + 663 WP_000045163 hypothetical protein -
HUZ79_RS27960 (HUZ79_27900) 48268..48411 + 144 WP_023356351 hypothetical protein -
HUZ79_RS27965 (HUZ79_27905) 48424..48771 + 348 WP_001102160 hypothetical protein -
HUZ79_RS27970 (HUZ79_27910) 48914..49195 + 282 WP_099360026 type IV conjugative transfer system protein TraL traL
HUZ79_RS27975 (HUZ79_27915) 49192..49818 + 627 WP_001049718 TraE/TraK family type IV conjugative transfer system protein traE
HUZ79_RS27980 (HUZ79_27920) 49802..50719 + 918 WP_000839951 type-F conjugative transfer system secretin TraK traK
HUZ79_RS27985 (HUZ79_27925) 50716..52032 + 1317 WP_000595204 TraB/VirB10 family protein traB
HUZ79_RS27990 (HUZ79_27930) 52029..52607 + 579 WP_000794193 type IV conjugative transfer system lipoprotein TraV traV
HUZ79_RS27995 (HUZ79_27935) 52611..53003 + 393 WP_012766327 TraA family conjugative transfer protein -
HUZ79_RS28000 (HUZ79_27940) 53067..54269 - 1203 WP_081843999 ISL3-like element ISAeme19 family transposase -
HUZ79_RS28005 (HUZ79_27945) 54516..60002 + 5487 WP_079778880 Ig-like domain-containing protein -
HUZ79_RS28010 (HUZ79_27950) 60151..60858 + 708 WP_001259344 DsbC family protein -
HUZ79_RS28015 (HUZ79_27955) 60855..63302 + 2448 WP_000587426 type IV secretion system protein TraC virb4
HUZ79_RS28020 (HUZ79_27960) 63317..63634 + 318 WP_000364398 hypothetical protein -
HUZ79_RS28025 (HUZ79_27965) 63631..64161 + 531 WP_000672456 S26 family signal peptidase -
HUZ79_RS28030 (HUZ79_27970) 64124..65392 + 1269 WP_001553264 TrbC family F-type conjugative pilus assembly protein traW
HUZ79_RS28035 (HUZ79_27975) 65389..66066 + 678 WP_000577801 EAL domain-containing protein -
HUZ79_RS28040 (HUZ79_27980) 66060..67064 + 1005 WP_000911304 TraU family protein traU
HUZ79_RS28045 (HUZ79_27985) 67215..69953 + 2739 WP_001256493 conjugal transfer mating pair stabilization protein TraN traN
HUZ79_RS28050 (HUZ79_27990) 69992..70852 - 861 WP_000679674 hypothetical protein -
HUZ79_RS28055 (HUZ79_27995) 70964..71614 - 651 WP_181248424 hypothetical protein -
HUZ79_RS28060 (HUZ79_28000) 71905..72225 + 321 WP_000547567 hypothetical protein -
HUZ79_RS28065 (HUZ79_28005) 72536..72721 + 186 WP_001186892 hypothetical protein -
HUZ79_RS28070 (HUZ79_28010) 72942..73910 + 969 WP_001553257 AAA family ATPase -
HUZ79_RS28075 (HUZ79_28015) 73922..74827 + 906 WP_000739141 hypothetical protein -

Host bacterium

ID   5038 GenBank   NZ_CP055012
Plasmid name   SWHEFF_72|unnamed2 Incompatibility group   IncA/C
Plasmid size   234910 bp Coordinate of oriT [Strand]   45453..45603 [+]
Host baterium   Klebsiella quasipneumoniae strain SWHEFF_72

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR, merR2, arsR, arsD, arsA, arsB, arsC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -