Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104584
Name   oriT_SWHE1|unnamed1 in_silico
Organism   Klebsiella pneumoniae strain SWHE1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP055067 (156494..156543 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_SWHE1|unnamed1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3300 GenBank   WP_023307532
Name   traI_HUZ64_RS26435_SWHE1|unnamed1 insolico UniProt ID   A0A2R4ND60
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190476.66 Da        Isoelectric Point: 6.4600

>WP_023307532.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRAMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELACAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALITIEQVTPEQVPRKEGVWAPGSSVVEFTPKQEKAIE
KALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRTLNGMIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSDGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASERYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRIWADPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1433-1555)

AAA domain (AAA_30)

(971-1154)

TrwC relaxase (TrwC)

(10-284)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)


  Protein structure



No available structure.




T4CP

ID   3239 GenBank   WP_065803682
Name   traD_HUZ64_RS26440_SWHE1|unnamed1 insolico UniProt ID   _
Length   769 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 769 a.a.        Molecular weight: 85823.79 Da        Isoelectric Point: 5.0577

>WP_065803682.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFANGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTVWCGEQLWTSIVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARTLFMPDAPASGPADTNSHAGEQPEPISQPA
PADMTVSPAPVKAPPTTKSPAAEPSVRATEPPVLRVTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 128853..157101

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ64_RS26440 (HUZ64_26355) 128853..131162 - 2310 WP_065803682 type IV conjugative transfer system coupling protein TraD virb4
HUZ64_RS26445 (HUZ64_26360) 131524..132255 - 732 Protein_140 conjugal transfer complement resistance protein TraT -
HUZ64_RS26450 (HUZ64_26370) 132445..132972 - 528 WP_024623127 conjugal transfer protein TraS -
HUZ64_RS26455 (HUZ64_26375) 132978..135827 - 2850 WP_065811049 conjugal transfer mating-pair stabilization protein TraG traG
HUZ64_RS26460 (HUZ64_26380) 135827..137206 - 1380 WP_011977731 conjugal transfer pilus assembly protein TraH traH
HUZ64_RS26465 (HUZ64_26385) 137184..137627 - 444 WP_015065638 F-type conjugal transfer protein TrbF -
HUZ64_RS26470 (HUZ64_26390) 137672..138229 - 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HUZ64_RS26475 (HUZ64_26395) 138201..138440 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
HUZ64_RS26480 (HUZ64_26400) 138451..139203 - 753 WP_015065637 type-F conjugative transfer system pilin assembly protein TraF traF
HUZ64_RS26485 (HUZ64_26405) 139224..139550 - 327 WP_004152685 hypothetical protein -
HUZ64_RS27310 139596..139781 - 186 WP_023307535 hypothetical protein -
HUZ64_RS26490 (HUZ64_26410) 139778..140014 - 237 WP_023307536 conjugal transfer protein TrbE -
HUZ64_RS26495 (HUZ64_26415) 140046..142001 - 1956 WP_015065636 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HUZ64_RS26500 (HUZ64_26420) 142060..142698 - 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
HUZ64_RS26505 (HUZ64_26425) 142711..143670 - 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
HUZ64_RS26510 (HUZ64_26430) 143714..144340 - 627 WP_015065633 type-F conjugative transfer system protein TraW traW
HUZ64_RS26515 (HUZ64_26435) 144340..144729 - 390 WP_004152506 type-F conjugative transfer system protein TrbI -
HUZ64_RS26520 (HUZ64_26440) 144729..147368 - 2640 WP_016528865 type IV secretion system protein TraC virb4
HUZ64_RS26525 (HUZ64_26445) 147440..147838 - 399 WP_015065631 hypothetical protein -
HUZ64_RS26530 (HUZ64_26450) 147863..148267 - 405 WP_023320104 hypothetical protein -
HUZ64_RS26535 (HUZ64_26455) 148334..148651 - 318 WP_015065629 hypothetical protein -
HUZ64_RS26540 (HUZ64_26460) 148652..148870 - 219 WP_004171484 hypothetical protein -
HUZ64_RS26545 (HUZ64_26465) 148894..149184 - 291 WP_032426790 hypothetical protein -
HUZ64_RS26550 149213..149611 - 399 WP_223177007 hypothetical protein -
HUZ64_RS26555 (HUZ64_26475) 149767..150336 - 570 WP_015065628 type IV conjugative transfer system lipoprotein TraV traV
HUZ64_RS26560 (HUZ64_26480) 150356..150511 - 156 WP_153587559 hypothetical protein -
HUZ64_RS26565 (HUZ64_26485) 150542..150961 - 420 Protein_165 conjugal transfer pilus-stabilizing protein TraP -
HUZ64_RS26570 (HUZ64_26490) 150954..152378 - 1425 WP_040228261 F-type conjugal transfer pilus assembly protein TraB traB
HUZ64_RS26575 (HUZ64_26495) 152378..153118 - 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
HUZ64_RS26580 (HUZ64_26500) 153105..153671 - 567 WP_004152602 type IV conjugative transfer system protein TraE traE
HUZ64_RS26585 (HUZ64_26505) 153691..153996 - 306 WP_065811051 type IV conjugative transfer system protein TraL traL
HUZ64_RS26590 (HUZ64_26510) 154010..154378 - 369 WP_004178060 type IV conjugative transfer system pilin TraA -
HUZ64_RS26595 (HUZ64_26515) 154432..154803 - 372 WP_004208838 TraY domain-containing protein -
HUZ64_RS26600 (HUZ64_26520) 154887..155582 - 696 WP_015065624 transcriptional regulator TraJ family protein -
HUZ64_RS26605 (HUZ64_26525) 155789..156181 - 393 WP_004206766 conjugal transfer relaxosome DNA-binding protein TraM -
HUZ64_RS26610 (HUZ64_26530) 156616..157101 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
HUZ64_RS26615 (HUZ64_26535) 157134..157448 - 315 WP_001568110 DUF5983 family protein -
HUZ64_RS26620 (HUZ64_26540) 157496..158317 - 822 WP_015065622 DUF932 domain-containing protein -
HUZ64_RS26625 (HUZ64_26545) 159151..159564 - 414 WP_004182074 helix-turn-helix domain-containing protein -
HUZ64_RS26630 (HUZ64_26550) 159565..159843 - 279 WP_004152721 helix-turn-helix transcriptional regulator -
HUZ64_RS26635 (HUZ64_26555) 159833..160153 - 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
HUZ64_RS26640 (HUZ64_26560) 160234..160458 - 225 WP_004152719 hypothetical protein -
HUZ64_RS26645 (HUZ64_26565) 160469..160681 - 213 WP_021313197 hypothetical protein -
HUZ64_RS26650 (HUZ64_26570) 160742..161098 - 357 WP_004152717 hypothetical protein -
HUZ64_RS26655 (HUZ64_26575) 161734..162084 - 351 WP_015065620 hypothetical protein -

Host bacterium

ID   5023 GenBank   NZ_CP055067
Plasmid name   SWHE1|unnamed1 Incompatibility group   IncFIB
Plasmid size   181158 bp Coordinate of oriT [Strand]   156494..156543 [+]
Host baterium   Klebsiella pneumoniae strain SWHE1

Cargo genes

Drug resistance gene   catA2, sul2, aph(3'')-Ib, aph(6)-Id, aph(3')-Ia, aac(3)-IId, floR, aac(6')-Ib-cr, ARR-3, dfrA27, aadA16, qacE, sul1, qnrB91, mph(A), tet(A)
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9