Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104560
Name   oriT_P281972|unnamed in_silico
Organism   Salmonella enterica strain P281972
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP092076 (15435..15488 [-], 54 nt)
oriT length   54 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 54 nt

>oriT_P281972|unnamed
CACAAGCATTGTAACATGCCCGGAACGGGCTTGTGTACAAAGCTTATCGTGCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3287 GenBank   WP_238728179
Name   Relaxase_MC377_RS23260_P281972|unnamed insolico UniProt ID   _
Length   1400 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1400 a.a.        Molecular weight: 160318.02 Da        Isoelectric Point: 5.1459

>WP_238728179.1 MULTISPECIES: LPD7 domain-containing protein [Enterobacteriaceae]
MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTTDKIINSIEDNKQDRYLHITLSFHESH
VSNEMLKAVVDDYKKLLMNAYHPEEYSFYAEAHLPKIRHIQDNSTGELIERKPHIHIVIPKVNLVTGDKL
DPVGLIGKGHTIEQLDAIQEHINNKYNLASPKDFPRKNSDYSVILKRVKADLDVERNSAFKNELNYRIEK
EKVSDYAVFKQIVAEYGEVRIRNEGKASEYIAVKLPGDKKFTNLKSPLFRKNYIEEGKLTLQKPTPKQIE
KRLNTWVNKTSHEIKHVFLNASVRKDYTSLSEAEQIEFLKERIKEYDSREKLNERNSQQTSGRAGGYKSC
PKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSISVLSDISQRDLAEQLPQREHPGQDVRRDYDRQF
TESGIKSLERSSFLCETMFQTLNEAAEKNEITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGS
PRFSVGNRNMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLNLSEK
TLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQYHADWHK
DKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSKLKDLVMDKQDSRIVYRDQE
SEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQ
QMMLELKAELQGNKFEQVETQENSQESESRIEKGDALKEQATEQEQATEQAQTTEQAQATEQAQVAEQAQ
VAEQAQVAEQATSSYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFDVA
SFDNENDAKEFCKIVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDLNEGIVFHSKDA
AIQCYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEGALVTFDRPFEIIANSYEEYRTPV
YAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHGLNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQ
AGDSSRESPEEILASISANEHMISGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYRLAVAGKHGLER
GDAVARVDVTEQQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLN
AEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPE
DGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDALGKQFVQAREDHQESQQQRDDSQDMH

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(691-773)

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-262)


  Protein structure



No available structure.




T4CP

ID   3226 GenBank   WP_193475645
Name   t4cp2_MC377_RS23370_P281972|unnamed insolico UniProt ID   _
Length   652 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 652 a.a.        Molecular weight: 73448.99 Da        Isoelectric Point: 9.1391

>WP_193475645.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVAIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDNGKDLATWMGQEIENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(127-591)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33523..57179

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC377_RS23315 (MC377_23315) 30422..30775 - 354 WP_157909760 pilus assembly protein -
MC377_RS23615 31277..31498 + 222 Protein_39 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MC377_RS23320 (MC377_23320) 31495..32871 - 1377 WP_000750519 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MC377_RS23325 (MC377_23325) 32884..33519 - 636 WP_044704741 A24 family peptidase -
MC377_RS23330 (MC377_23330) 33523..34005 - 483 WP_001258095 lytic transglycosylase domain-containing protein virB1
MC377_RS23335 (MC377_23335) 34072..34629 - 558 WP_000095047 type 4 pilus major pilin -
MC377_RS23340 (MC377_23340) 34674..35783 - 1110 WP_000974903 type II secretion system F family protein -
MC377_RS23345 (MC377_23345) 35774..37312 - 1539 WP_000466225 ATPase, T2SS/T4P/T4SS family virB11
MC377_RS23350 (MC377_23350) 37337..37831 - 495 WP_000912553 type IV pilus biogenesis protein PilP -
MC377_RS23355 (MC377_23355) 37815..39125 - 1311 WP_225088990 type 4b pilus protein PilO2 -
MC377_RS23360 (MC377_23360) 39176..40819 - 1644 WP_001035589 PilN family type IVB pilus formation outer membrane protein -
MC377_RS23365 (MC377_23365) 40812..41330 - 519 WP_001220540 sigma 54-interacting transcriptional regulator virb4
MC377_RS23370 (MC377_23370) 41377..43335 - 1959 WP_193475645 type IV secretory system conjugative DNA transfer family protein -
MC377_RS23375 (MC377_23375) 43351..44406 - 1056 WP_238728176 P-type DNA transfer ATPase VirB11 virB11
MC377_RS23380 (MC377_23380) 44425..45564 - 1140 WP_000790641 TrbI/VirB10 family protein virB10
MC377_RS23385 (MC377_23385) 45554..46255 - 702 WP_000274524 TrbG/VirB9 family P-type conjugative transfer protein -
MC377_RS23390 (MC377_23390) 46321..47055 - 735 WP_000432283 type IV secretion system protein virB8
MC377_RS23395 (MC377_23395) 47055..47189 - 135 WP_000701233 hypothetical protein -
MC377_RS23400 (MC377_23400) 47221..49578 - 2358 WP_000548950 VirB4 family type IV secretion system protein virb4
MC377_RS23405 (MC377_23405) 49584..49904 - 321 WP_000362080 VirB3 family type IV secretion system protein virB3
MC377_RS23410 (MC377_23410) 49975..50265 - 291 WP_000865479 conjugal transfer protein -
MC377_RS23415 (MC377_23415) 50265..50849 - 585 WP_001177114 lytic transglycosylase domain-containing protein virB1
MC377_RS23420 (MC377_23420) 50870..51268 - 399 WP_001153668 hypothetical protein -
MC377_RS23425 (MC377_23425) 51608..52045 - 438 WP_193475646 type IV pilus biogenesis protein PilM -
MC377_RS23430 (MC377_23430) 52051..53286 - 1236 WP_000733400 toxin co-regulated pilus biosynthesis Q family protein -
MC377_RS23435 (MC377_23435) 53289..53576 - 288 WP_001326593 TrbM/KikA/MpfK family conjugal transfer protein -
MC377_RS23440 (MC377_23440) 53748..54383 - 636 WP_238728177 hypothetical protein -
MC377_RS23445 (MC377_23445) 54464..55222 + 759 WP_224482110 DUF5710 domain-containing protein -
MC377_RS23450 (MC377_23450) 55300..55536 - 237 WP_000750964 EexN family lipoprotein -
MC377_RS23455 (MC377_23455) 55540..56187 - 648 WP_000653673 type IV secretion system protein -
MC377_RS23460 (MC377_23460) 56193..57179 - 987 WP_001028544 type IV secretion system protein virB6
MC377_RS23465 (MC377_23465) 57183..57440 - 258 WP_000739144 hypothetical protein -
MC377_RS23470 (MC377_23470) 57437..57739 - 303 WP_001360345 hypothetical protein -
MC377_RS23475 (MC377_23475) 58154..58615 - 462 WP_001243166 hypothetical protein -
MC377_RS23480 (MC377_23480) 58626..58796 - 171 WP_000550720 hypothetical protein -
MC377_RS23485 (MC377_23485) 58800..59243 - 444 WP_000964330 NfeD family protein -
MC377_RS23490 (MC377_23490) 59617..60570 - 954 WP_072089442 SPFH domain-containing protein -
MC377_RS23495 (MC377_23495) 60616..60810 - 195 WP_001127356 DUF1187 family protein -
MC377_RS23500 (MC377_23500) 60803..61309 - 507 WP_238728178 CaiF/GrlA family transcriptional regulator -

Host bacterium

ID   4999 GenBank   NZ_CP092076
Plasmid name   P281972|unnamed Incompatibility group   IncI2
Plasmid size   62386 bp Coordinate of oriT [Strand]   15435..15488 [-]
Host baterium   Salmonella enterica strain P281972

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -