Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104398
Name   oriT_pK520-MPH in_silico
Organism   Aeromonas allosaccharophila strain K520
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP118990 (40063..40192 [+], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_pK520-MPH
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3208 GenBank   WP_244776049
Name   mobH_PYU98_RS24555_pK520-MPH insolico UniProt ID   _
Length   984 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 984 a.a.        Molecular weight: 107275.56 Da        Isoelectric Point: 4.7871

>WP_244776049.1 MULTISPECIES: MobH family relaxase [Aeromonas]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKTQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTKPAE
GTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEKAIQ
MLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKLYLE
VDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(22-328)


  Protein structure



No available structure.




T4CP

ID   3120 GenBank   WP_011899407
Name   traD_PYU98_RS24560_pK520-MPH insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.



ID   3121 GenBank   WP_270802391
Name   traC_PYU98_RS24805_pK520-MPH insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 91335.25 Da        Isoelectric Point: 5.7249

>WP_270802391.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLDGALIPEHMRASGVIPVLAYSEDEQIFLMDDQSAGFGFICEPLCGADEKVQERMNGFLNQE
YPSKTTIQFLLFRSPDINQEMYQMQGLRDGFRHSLLSSVIQERISYLQHHTTERIMARTSKGIYDNGLIQ
DLKLFVTCKVPIKGNTPNATELAELDQLKTKVESSLQTVGLRPRTMTAVNYVRIMGTLLNWGPDASWRHD
ASLWETDKPICEQLFDYGTDLEVSKNGIRLGDYHAKVLSAKKLPDIAYFGDAIAYAGDLTGGNSSIKENY
IVVTNVFFPEVESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTVYESLKEGAKPVKISYSLVIF
APTKERVESAATAARSIWRESRFELMEDRFVALPIFLNSLPFCTDRDAVRDLFRYKTMTSEQAAVVLPVF
GEWKGTGTFHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELICSYLSEGAQVWVIDAGKS
YQKMSEMLKGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASSKGLLVEWQISALKQVLSSIWE
EKGTEMKVDDIAERCLEGADQRMKDVGQQLYAFTSGGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRAGRLALSDGGFHTLKTVHTIQGVYSEIFIKSKAGMGVGRLI
VGDFQKLLYSTDPADVNAIDQFVNQGLTVPDAIKAVMQARRQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-808)

Family of unknown function (DUF5934) (DUF5934)

(285-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 38142..48043

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PYU98_RS24550 (PYU98_24550) 34321..34935 - 615 WP_011899409 hypothetical protein -
PYU98_RS24555 (PYU98_24555) 35185..38139 + 2955 WP_244776049 MobH family relaxase -
PYU98_RS24560 (PYU98_24560) 38142..40007 + 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
PYU98_RS24565 (PYU98_24565) 40018..40602 + 585 WP_073536825 hypothetical protein -
PYU98_RS24570 (PYU98_24570) 40559..41188 + 630 WP_073536826 DUF4400 domain-containing protein tfc7
PYU98_RS24575 (PYU98_24575) 41333..41722 + 390 WP_244776048 hypothetical protein -
PYU98_RS24580 (PYU98_24580) 41972..42172 + 201 WP_245147270 hypothetical protein -
PYU98_RS24585 (PYU98_24585) 42188..42481 + 294 WP_219935016 hypothetical protein -
PYU98_RS24590 (PYU98_24590) 42498..42692 + 195 WP_103859340 hypothetical protein -
PYU98_RS24595 (PYU98_24595) 42705..43043 + 339 WP_244776046 hypothetical protein -
PYU98_RS24600 (PYU98_24600) 43057..43527 + 471 WP_199416642 hypothetical protein -
PYU98_RS24605 (PYU98_24605) 43576..43821 + 246 WP_011899397 hypothetical protein -
PYU98_RS24610 (PYU98_24610) 44356..44637 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
PYU98_RS24615 (PYU98_24615) 44634..45269 + 636 WP_244776045 type IV conjugative transfer system protein TraE traE
PYU98_RS24620 (PYU98_24620) 45253..46158 + 906 WP_244776044 type-F conjugative transfer system secretin TraK traK
PYU98_RS24625 (PYU98_24625) 46161..47480 + 1320 WP_219935051 TraB/VirB10 family protein traB
PYU98_RS24630 (PYU98_24630) 47477..48043 + 567 WP_073536829 type IV conjugative transfer system lipoprotein TraV -
PYU98_RS24635 (PYU98_24635) 48134..48460 + 327 WP_050804919 TraA family conjugative transfer protein -

Host bacterium

ID   4837 GenBank   NZ_CP118990
Plasmid name   pK520-MPH Incompatibility group   -
Plasmid size   208934 bp Coordinate of oriT [Strand]   40063..40192 [+]
Host baterium   Aeromonas allosaccharophila strain K520

Cargo genes

Drug resistance gene   msr(E), mph(E)
Virulence gene   galU
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -