Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104383 |
| Name | oriT_pL2395_2  |
| Organism | Citrobacter koseri strain L2395 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP118929 (35691..35796 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pL2395_2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3204 | GenBank | WP_275055907 |
| Name | Relaxase_PX343_RS23475_pL2395_2 |
UniProt ID | _ |
| Length | 662 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 662 a.a. Molecular weight: 75694.54 Da Isoelectric Point: 10.0285
>WP_275055907.1 TraI/MobA(P) family conjugative relaxase [Citrobacter koseri]
MRWMVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVIST
PVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKF
TLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYV
VNDRQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDD
VHKTFAEIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVF
KTAYTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVR
HSVSDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFA
YSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHI
VLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAY
AQNEKHRLIREQNNSKNEMQFRSENDDKFNPK
MRWMVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVIST
PVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKF
TLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYV
VNDRQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDD
VHKTFAEIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVF
KTAYTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVR
HSVSDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFA
YSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHI
VLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAY
AQNEKHRLIREQNNSKNEMQFRSENDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 3118 | GenBank | WP_011091092 |
| Name | t4cp2_PX343_RS23215_pL2395_2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 38592..57970
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PX343_RS23455 (PX343_23455) | 33676..33987 | + | 312 | WP_069722402 | hypothetical protein | - |
| PX343_RS23460 (PX343_23460) | 34120..34656 | + | 537 | WP_011091066 | hypothetical protein | - |
| PX343_RS23465 (PX343_23465) | 35157..35522 | - | 366 | WP_039592107 | hypothetical protein | - |
| PX343_RS23470 (PX343_23470) | 35588..36115 | + | 528 | WP_275055906 | plasmid mobilization protein MobA | - |
| PX343_RS23475 (PX343_23475) | 36093..38081 | + | 1989 | WP_275055907 | TraI/MobA(P) family conjugative relaxase | - |
| PX343_RS23480 (PX343_23480) | 38095..38595 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| PX343_RS23485 (PX343_23485) | 38592..39371 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| PX343_RS23490 (PX343_23490) | 39382..40545 | + | 1164 | WP_040027864 | plasmid transfer ATPase TraJ | virB11 |
| PX343_RS23495 (PX343_23495) | 40535..40795 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| PX343_RS23500 (PX343_23500) | 40820..44347 | + | 3528 | WP_275055908 | LPD7 domain-containing protein | - |
| PX343_RS23505 (PX343_23505) | 44313..44825 | + | 513 | WP_011091071 | hypothetical protein | traL |
| PX343_RS23510 (PX343_23510) | 44826..45038 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| PX343_RS23515 (PX343_23515) | 45010..45420 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| PX343_RS23520 (PX343_23520) | 45488..46201 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| PX343_RS23525 (PX343_23525) | 46210..47361 | + | 1152 | WP_116961601 | DotH/IcmK family type IV secretion protein | traN |
| PX343_RS23530 (PX343_23530) | 47373..48722 | + | 1350 | WP_116961602 | conjugal transfer protein TraO | traO |
| PX343_RS23535 (PX343_23535) | 48734..49438 | + | 705 | WP_116961603 | conjugal transfer protein TraP | traP |
| PX343_RS23540 (PX343_23540) | 49462..49992 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| PX343_RS23545 (PX343_23545) | 50009..50398 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| PX343_RS23550 (PX343_23550) | 50444..50938 | + | 495 | WP_011154473 | hypothetical protein | - |
| PX343_RS23555 (PX343_23555) | 50935..52647 | + | 1713 | WP_275055909 | hypothetical protein | traU |
| PX343_RS23560 (PX343_23560) | 52692..53984 | + | 1293 | WP_275055910 | hypothetical protein | traU |
| PX343_RS23565 (PX343_23565) | 53981..55189 | + | 1209 | WP_159310015 | conjugal transfer protein TraW | traW |
| PX343_RS23570 (PX343_23570) | 55186..55782 | + | 597 | WP_015060003 | hypothetical protein | - |
| PX343_RS23575 (PX343_23575) | 55775..57970 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| PX343_RS23580 (PX343_23580) | 57972..58625 | + | 654 | WP_015062835 | hypothetical protein | - |
| PX343_RS23585 (PX343_23585) | 58706..58936 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 4822 | GenBank | NZ_CP118929 |
| Plasmid name | pL2395_2 | Incompatibility group | IncL/M |
| Plasmid size | 59233 bp | Coordinate of oriT [Strand] | 35691..35796 [+] |
| Host baterium | Citrobacter koseri strain L2395 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |