Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104374
Name   oriT_pCTYH.Ch1_2 in_silico
Organism   Ensifer adhaerens strain CTYH.Ch1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP118612 (554944..554975 [+], 32 nt)
oriT length   32 nt
IRs (inverted repeats)      17..22, 27..32  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 32 nt

>oriT_pCTYH.Ch1_2
GAAGGGCGCAATTATACGTCGCTGTTGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3196 GenBank   WP_275027580
Name   traA_PWG15_RS35560_pCTYH.Ch1_2 insolico UniProt ID   _
Length   1101 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1101 a.a.        Molecular weight: 121242.64 Da        Isoelectric Point: 10.0236

>WP_275027580.1 Ti-type conjugative transfer relaxase TraA [Ensifer adhaerens]
MAVPHFSVNIVARGAGRSAVMSAAYRHCAKMDFEREARGVDYRAKQGLLHEEFVVPADAPAWLSSMIADR
SVSGASEAFWNRVEEFEKRADAQLAKDLTIALPIELTPEQNIGLVREFVERQILSKGMVADWVYHDAPGN
PHVHLMATLRPLTDEGFGAKKVALLAPDGRPLRNDMGKIVYELWAGGVDDFNALRDGWFACQNRHLALAG
LDIRVDGRSYESQGITVAPTIHLGVGAKAIERKSTASQMPASLERLELQEALRQANARRIERRPQIVLDM
ITREKSVFDERDVAKILHRYIDDADLFGSLMLRVLRDPSALRLTAEGTDLGTGLYSPARYTTRELIRLEA
EMVNRALWLARRTSHGVRGALLEATFSKNRGLSDEQQAAIEHLTTGAALAAVVGRAGAGKTTMMKAAREA
WQAAGYRVVGGALAGKAAEGLEKEAGIASRTLSSWERRWAQGTDCLDEKTVFVLDEAGMVSSRQMAFFVE
AVVRSGAKLVLVGDPEQLQPIEAGAAFRAIGDRVGYAELQTIYRQRAPWMRSASLDLARGKIGEAIDAYR
ANGKIVGAELKSEAIERLITDWDREHDLTKTSLMLAHLRRDVRLLNSLARARLVERGVVGEGAAFRTADG
NRNFSAGDQIVFLKNDGILGVKNGMLGRVVEAGPGRFIASIGEGTHRRDVTVEQRLYNNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHRDELAVYYGRRSFAKSGGLVPILSRRNAKETTLDYEARPLY
RQALRFAEARGLHLMSVARTVVRDRLHWIVGQKQKLAMLGARLVDLASSIGLARGKLERAAPAIGAARPI
VSAITVFAKSVEQAVADRLAADLNLRERWQDVSMRFQLVYADPQVAFAAVKVDAMLKDTSLAATVLQELA
AEPQHFGALSGRTGMFASRSDKKDRDAALLNVPALARNLADYVRVRAETERRCLAEENAVRSKLAVEIPA
LSPVTRQALEQLREGLERDDQARHEPSPLPPSIRQELERFSAAVSQRFGERTFPQIAAKAPDGEVFAAVT
AGMAPQQKNEVRLAWDAMRTIQLLSARDRVAAGLKLSETQRQSQGKELRLK

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(382-567)

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(858-954)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(690-736)


  Protein structure



No available structure.




T4CP

ID   3109 GenBank   WP_275027575
Name   traG_PWG15_RS35545_pCTYH.Ch1_2 insolico UniProt ID   _
Length   637 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 637 a.a.        Molecular weight: 68606.28 Da        Isoelectric Point: 9.0825

>WP_275027575.1 Ti-type conjugative transfer system protein TraG [Ensifer adhaerens]
MGVSRIALAASPLALMALALVTMEGAGDWLSSFAGREAGRLALIRAGVAIPSVSAAVVGAIFLFAAAGSA
NIRFAGWGAFGGGLTAAAVALIDGFVDTSLAQSWQFHTLDFEPAAVVGAVAALLSSGFALRVAMIGNAAF
PKSAPKRVRGKRAIYGEAEWMKLSCAKRLFPEVAGIVIGEAYRVDEDGVAAIPFRADKPETWGAGGRSPL
LCFDGSFGSSHGIVFAGSGGFKTTSVTVPTALKWGSTLVVLDPSNEVAPMVIDHRKGAGRDVRILDPKKP
DAGFNALDWIGRYGRSKEEDIAAVASWIMSDSGRAIGVRDDFFRASALQLLTALIADVCLSGHTRKEDQT
LRQVRRNLAEPEPKLRERLQAIHANSSSGFVRENVAAFVNMTPETFSGVYANAVKETHWLSYENYAGLVS
SKSFSTEQLLAGSLDVFINIDLKTLETHSGLARVIIGSFVNAIYNRNRSGDDLALFLLDEVARLGYLRIL
ETARDTGRKYGISLTLIFQSIGQMRETFGGRDATSKWFESASWVSFAAINDPETAEYVSRRCGMTTVEVD
QVSRSFQSRGSSRTRSKQLAARPLIQPDEVLRMRADEQIVFTAGNAPLRCGRAIWFRREDMQGCVKGSRF
HAPSEPV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(160-629)

  Protein structure



No available structure.



ID   3110 GenBank   WP_275028018
Name   t4cp2_PWG15_RS35850_pCTYH.Ch1_2 insolico UniProt ID   _
Length   816 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 816 a.a.        Molecular weight: 91314.23 Da        Isoelectric Point: 5.6099

>WP_275028018.1 conjugal transfer protein TrbE [Ensifer adhaerens]
MVNLRRFRSPDPSFADLVPYAGLVDNGIILLKDGSLMAGWYFAGPDSESATDLERNEISRQINAVLARLG
SGWMIQVEAVRVEATAYPSAERSHFPDPVTRAIEAERRAHFEREGTHFESRHALILTQRPIEQRRSGLGK
YVYADPESRKRGFADTALDLFRTRVREVEQYLANWISLRRMATRELNPADDGRKERFDELFQFIRFCISG
ENHPIRLPDIPFYLDWLVTAEFQHGLTPLVDNRHLGVVAIDGLPSESWPGILNGLDRLPMAYRWSSRFIF
LDAQEARERLERTRKKWQQKVRPFFDQLFQTEGRSIDQDAIAMVAEVEDAIAQASSELVAYGYYTPVVIV
FGADEPEVRDKCELVRQAVQVEGFGARIETLNATEAYLGSLPGNWYCNVREPLINTRNLADLIPLNTVWA
GNPFAPCPYLPPNSPPLLQVASGSTPFRFNLHVDDIGHTLVFGPTGSGKSTLLALIAAQFRRYVGAQVFI
FDKGRSMLPLTLAVGGGHYEIGADGEAPGLCFCPLLDLEGEGDRAWACQWVETLAELQGASIGPEERNAI
SRQITLMATASGRSLSDFVSGVQLKAIKDALQHYTIDGPLGALIDAEIDTLSLGAFEAFEIEHLMVLGER
SLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPLFRERIREWLKVLRKANCAVVLATQSISDAERSG
IIDVLRESCPTKICLPNAAARQPGAREFYERMGFNDRQIEIVAASTPKRDYYVTSPEGRRLFDMALGPLA
LAFVGAAGKDDLKRIRALHLEHGRSWPVEWLKLRGIPDAETLLADS

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(193-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 613511..622792

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWG15_RS35800 (PWG15_35795) 609028..610374 - 1347 WP_275027631 amidohydrolase -
PWG15_RS35805 (PWG15_35800) 610391..611398 - 1008 WP_275028015 response regulator transcription factor -
PWG15_RS35810 (PWG15_35805) 611895..612626 + 732 WP_275027632 winged helix-turn-helix domain-containing protein -
PWG15_RS35815 (PWG15_35810) 612780..613496 + 717 WP_275027633 autoinducer binding domain-containing protein -
PWG15_RS35820 (PWG15_35815) 613511..614791 - 1281 WP_275027634 IncP-type conjugal transfer protein TrbI virB10
PWG15_RS35825 (PWG15_35820) 614801..615241 - 441 WP_275027635 conjugal transfer protein TrbH -
PWG15_RS35830 (PWG15_35825) 615246..616034 - 789 WP_275028016 P-type conjugative transfer protein TrbG virB9
PWG15_RS35835 (PWG15_35830) 616076..616738 - 663 WP_275027636 conjugal transfer protein TrbF virB8
PWG15_RS35840 (PWG15_35835) 616759..617889 - 1131 WP_275027637 P-type conjugative transfer protein TrbL virB6
PWG15_RS35845 (PWG15_35840) 617943..618737 - 795 WP_275027638 P-type conjugative transfer protein TrbJ virB5
PWG15_RS35850 (PWG15_35845) 618709..621159 - 2451 WP_275028018 conjugal transfer protein TrbE virb4
PWG15_RS35855 (PWG15_35850) 621168..621467 - 300 WP_275027639 conjugal transfer protein TrbD virB3
PWG15_RS35860 (PWG15_35855) 621460..621837 - 378 WP_275027640 TrbC/VirB2 family protein virB2
PWG15_RS35865 (PWG15_35860) 621827..622792 - 966 WP_275027641 P-type conjugative transfer ATPase TrbB virB11
PWG15_RS35870 (PWG15_35865) 622789..623424 - 636 WP_275027642 acyl-homoserine-lactone synthase -

Host bacterium

ID   4813 GenBank   NZ_CP118612
Plasmid name   pCTYH.Ch1_2 Incompatibility group   -
Plasmid size   623840 bp Coordinate of oriT [Strand]   554944..554975 [+]
Host baterium   Ensifer adhaerens strain CTYH.Ch1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   hdt
Symbiosis gene   fixA, fixB
Anti-CRISPR   -