Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104368
• Name: oriT_pShVSI35_4
• Organism: Staphylococcus haemolyticus strain VSI35
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP118064 (330..427 [+], 98 nt)
• oriT length: 98 nt
• IRs (inverted repeats): _
• Location of nic site: 66..67
• Conserved sequence flanking the nic site: GATTGGTCGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 98 nt

>oriT_pShVSI35_4
TCAGCTGAAACTTTAAATATGAGTGTGCCTACTTTCGTTAAGAAAAAGGCACAAGGCAGTCGATTGGTCGCACCTAAATTTGATAAAGAAACGCGACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_PUW68_RS12235_pShVSI35_4

• ID: 3192
• GenBank: WP_086040258
• Name: Rep_trans_PUW68_RS12235_pShVSI35_4
• UniProt ID: _
• Length: 311 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 311 a.a.
• Molecular weight: 37358.58 Da
• Isoelectric Point: 8.7110

>WP_086040258.1 MULTISPECIES: replication initiation factor domain-containing protein [Staphylococcus]
MSTENYSNYLQNKDLDNFSKTGYSNSRLDAHTVCISKPKISFDAMTIVGNLNKNNAQKLSEFMSVEPQIR
LWDILQTKFKAKALQEKVYIEYDKVKADAWDRRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDL
AFDFEYDLSSYYAMNDKAVKKTVFYGTNGMPETKYFGVRDSDRFIRIYNKKKERKDNADIEISSEHLWRV
EIELKRNMVDYWNDCFNDLHILKPDWKSVERTSDRAIVFMLLNDEEEWGKLHRNSRTKYKKLIKEISPID
LTDLMKSTLKANEKQLQKQIDFWKREFKIWK

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (152-282)

Protein structure

No available structure.

Host bacterium

• ID: 4807
• GenBank: NZ_CP118064
• Plasmid name: pShVSI35_4
• Incompatibility group: -
• Plasmid size: 3781 bp
• Coordinate of oriT [Strand]: 330..427 [+]
• Host baterium: Staphylococcus haemolyticus strain VSI35

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -