Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104362
• Name: oriT_pSaVSI37_2
• Organism: Streptococcus anginosus strain VSI37
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP118056 (748..788 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pSaVSI37_2
CACTTTTACGAAGTAAAGTATAGTGCGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_PUW49_RS11000_pSaVSI37_2

• ID: 3189
• GenBank: WP_070654249
• Name: mobV_PUW49_RS11000_pSaVSI37_2
• UniProt ID: A0A1F0RJV6
• Length: 500 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 500 a.a.
• Molecular weight: 58376.37 Da
• Isoelectric Point: 6.6214

>WP_070654249.1 MULTISPECIES: MobV family relaxase [Lactobacillales]
MSYMVARMQKMKVGNLGGAHKHNERIFEKHSNKDIDTSRSHLNYELTGRDRSVSYERQIKDYVNENKSSK
RGIRKDAVLCDEWIITSDKEFFEKLSQEQTRKFFETAKNYFAENYGETNVAYASVHLDESTPHMHLGIVP
MRNGKLSSKVMFNREELKHIQEDLPKYMNEHGFELQRGKRDSKEQHLSVADYKEKMGREALNQELLDLGA
PKYWHTEEHRPATISEIQDYEDIGSLFADSDVTFKIREATLEERFKWLDTHRNDLRADLGRLEGVLEKKI
DEYGKIDSEASERLSELSELNSEVNSRQEEIKGLKSDSKRLSDDVVRLERAHREKTQLLVEQSSNLSKIS
YRDLDRRRVAKELQEELENATPKLFGDGFNFTSDFVGRLKTFVSDVVEKLEQAVNQNELLRNALAGMKEA
KSRLENSLSHAEWKNQQLETENKALKLENRELKFSKNLLDDLSEVITEKEATSLNKRLENLRETRELSRK
RHEPTKGRSI

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-194)

Protein structure

• AlphaFold DB: A0A1F0RJV6

Host bacterium

• ID: 4801
• GenBank: NZ_CP118056
• Plasmid name: pSaVSI37_2
• Incompatibility group: -
• Plasmid size: 4357 bp
• Coordinate of oriT [Strand]: 748..788 [-]
• Host baterium: Streptococcus anginosus strain VSI37

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -