Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104353
Name   oriT_pW6-2 in_silico
Organism   Enterococcus faecium strain W6
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP118549 (54053..54082 [+], 30 nt)
oriT length   30 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 30 nt

>oriT_pW6-2
AACGTAAGTTAGTGGGTATAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3184 GenBank   WP_227158223
Name   mobQ_PWA42_RS13425_pW6-2 insolico UniProt ID   _
Length   664 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 664 a.a.        Molecular weight: 77667.81 Da        Isoelectric Point: 9.2532

>WP_227158223.1 MULTISPECIES: MobQ family relaxase [Enterococcus]
MIQLAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR
QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHI
MLTMREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKD
RNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQ
DQNFTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISS
ERDMLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEE
SKKIFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMK
YIDELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRL
LSGNQLQKKRNLDKLIKQTKAKENQSLQRNIPLR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(21-245)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(292-397)


  Protein structure



No available structure.




T4CP

ID   3100 GenBank   WP_274875494
Name   t4cp2_PWA42_RS13380_pW6-2 insolico UniProt ID   _
Length   551 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 551 a.a.        Molecular weight: 63099.13 Da        Isoelectric Point: 4.7040

>WP_274875494.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MSSLLAESDGLILGKFPSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(33-456)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 31648..51587

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWA42_RS13310 (PWA42_13310) 27593..28702 - 1110 WP_223603198 ATP-binding protein -
PWA42_RS13315 (PWA42_13315) 29110..29504 - 395 Protein_24 hypothetical protein -
PWA42_RS13320 (PWA42_13320) 29553..30152 - 600 WP_142967644 site-specific integrase -
PWA42_RS13325 (PWA42_13325) 30576..31535 - 960 Protein_26 IS256-like element ISEfm2 family transposase -
PWA42_RS13330 (PWA42_13330) 31648..32541 - 894 WP_274875499 LPXTG cell wall anchor domain-containing protein prgC
PWA42_RS13335 (PWA42_13335) 32953..36051 - 3099 WP_129984717 type I restriction endonuclease subunit R -
PWA42_RS13340 (PWA42_13340) 36054..37223 - 1170 WP_231450768 restriction endonuclease subunit S -
PWA42_RS13345 (PWA42_13345) 37220..38767 - 1548 WP_108173618 type I restriction-modification system subunit M -
PWA42_RS13350 (PWA42_13350) 39260..39505 - 246 WP_108173619 restriction endonuclease subunit S -
PWA42_RS13355 (PWA42_13355) 39752..40129 + 378 WP_002339800 hypothetical protein -
PWA42_RS13360 (PWA42_13360) 40189..40542 - 354 WP_002301645 hypothetical protein -
PWA42_RS13365 (PWA42_13365) 40571..41539 - 969 WP_113795146 hypothetical protein -
PWA42_RS13370 (PWA42_13370) 41556..42500 - 945 WP_274875492 conjugal transfer protein TrbL family protein trsL
PWA42_RS13375 (PWA42_13375) 42500..43423 - 924 WP_274875493 hypothetical protein traP
PWA42_RS13380 (PWA42_13380) 43441..45096 - 1656 WP_274875494 VirD4-like conjugal transfer protein, CD1115 family -
PWA42_RS13385 (PWA42_13385) 45089..45520 - 432 WP_002338759 hypothetical protein -
PWA42_RS13390 (PWA42_13390) 45525..46076 - 552 WP_274875495 hypothetical protein -
PWA42_RS13395 (PWA42_13395) 46089..47198 - 1110 WP_002332647 lysozyme family protein orf14
PWA42_RS13400 (PWA42_13400) 47220..48572 - 1353 WP_274875496 conjugal transfer protein TraF trsF
PWA42_RS13405 (PWA42_13405) 48586..50547 - 1962 WP_231428364 TrsE protein virb4
PWA42_RS13410 (PWA42_13410) 50558..51187 - 630 WP_104888277 hypothetical protein trsD
PWA42_RS13415 (PWA42_13415) 51204..51587 - 384 WP_001083514 glycosyl transferase trsC
PWA42_RS13420 (PWA42_13420) 51606..51938 - 333 WP_000713503 CagC family type IV secretion system protein -
PWA42_RS13425 (PWA42_13425) 51962..53947 - 1986 WP_233701194 MobQ family relaxase -
PWA42_RS13430 (PWA42_13430) 54239..54538 + 300 WP_002325624 hypothetical protein -
PWA42_RS13435 (PWA42_13435) 54541..54798 + 258 WP_010717195 hypothetical protein -
PWA42_RS13440 (PWA42_13440) 54828..55004 - 177 WP_181728217 hypothetical protein -
PWA42_RS13445 (PWA42_13445) 55076..55756 - 681 WP_001015311 IS6-like element IS1216 family transposase -

Host bacterium

ID   4792 GenBank   NZ_CP118549
Plasmid name   pW6-2 Incompatibility group   -
Plasmid size   67732 bp Coordinate of oriT [Strand]   54053..54082 [+]
Host baterium   Enterococcus faecium strain W6

Cargo genes

Drug resistance gene   erm(A), optrA, fexA
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -