Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104351 |
| Name | oriT_2020CK-00206|unnamed3  |
| Organism | Enterobacter hormaechei strain 2020CK-00206 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP118555 (37117..37221 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_2020CK-00206|unnamed3
AGTACGGAACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGAACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3183 | GenBank | WP_004206924 |
| Name | Relaxase_PWP98_RS25175_2020CK-00206|unnamed3 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 3099 | GenBank | WP_004206885 |
| Name | t4cp2_PWP98_RS24925_2020CK-00206|unnamed3 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 40019..59215
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWP98_RS25155 (PWP98_25155) | 35103..35414 | + | 312 | WP_004187333 | hypothetical protein | - |
| PWP98_RS25160 (PWP98_25160) | 35547..36083 | + | 537 | WP_004206920 | hypothetical protein | - |
| PWP98_RS25165 (PWP98_25165) | 36584..36949 | - | 366 | WP_004206922 | hypothetical protein | - |
| PWP98_RS25170 (PWP98_25170) | 37225..37542 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| PWP98_RS25175 (PWP98_25175) | 37529..39508 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| PWP98_RS25180 (PWP98_25180) | 39522..40022 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| PWP98_RS25185 (PWP98_25185) | 40019..40798 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| PWP98_RS25190 (PWP98_25190) | 40809..41972 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| PWP98_RS25195 (PWP98_25195) | 41962..42222 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| PWP98_RS25200 (PWP98_25200) | 42247..45552 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| PWP98_RS25205 (PWP98_25205) | 45518..46030 | + | 513 | WP_011091071 | hypothetical protein | traL |
| PWP98_RS25210 (PWP98_25210) | 46031..46243 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| PWP98_RS25215 (PWP98_25215) | 46215..46625 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| PWP98_RS25220 (PWP98_25220) | 46693..47406 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| PWP98_RS25225 (PWP98_25225) | 47415..48566 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| PWP98_RS25230 (PWP98_25230) | 48578..49927 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| PWP98_RS25235 (PWP98_25235) | 49939..50643 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| PWP98_RS25240 (PWP98_25240) | 50667..51197 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| PWP98_RS25245 (PWP98_25245) | 51214..51603 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| PWP98_RS25250 (PWP98_25250) | 51649..52143 | + | 495 | WP_004187480 | hypothetical protein | - |
| PWP98_RS25255 (PWP98_25255) | 52140..55190 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| PWP98_RS25260 (PWP98_25260) | 55187..56395 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| PWP98_RS25265 (PWP98_25265) | 56392..57042 | + | 651 | WP_004187488 | hypothetical protein | - |
| PWP98_RS25270 (PWP98_25270) | 57035..59215 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| PWP98_RS25275 (PWP98_25275) | 59218..59871 | + | 654 | WP_004206935 | hypothetical protein | - |
| PWP98_RS25280 (PWP98_25280) | 59945..60175 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 4790 | GenBank | NZ_CP118555 |
| Plasmid name | 2020CK-00206|unnamed3 | Incompatibility group | IncL/M |
| Plasmid size | 60471 bp | Coordinate of oriT [Strand] | 37117..37221 [+] |
| Host baterium | Enterobacter hormaechei strain 2020CK-00206 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |