Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104349 |
| Name | oriT_2020CK-00216|unnamed2  |
| Organism | Enterobacter kobei strain 2020CK-00216 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP118562 (32944..33043 [-], 100 nt) |
| oriT length | 100 nt |
| IRs (inverted repeats) | 34..41, 44..51 (AGTGCGAT..ATCGCACT) 20..25, 39..44 (TAAATC..GATTTA) |
| Location of nic site | 62..63 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 100 nt
>oriT_2020CK-00216|unnamed2
TATTGAATGTTTTATTCTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATCAGTTTGGTAG
TATTGAATGTTTTATTCTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATCAGTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3182 | GenBank | WP_020323154 |
| Name | mobF_PXH70_RS23615_2020CK-00216|unnamed2 |
UniProt ID | A0A0R8CGS3 |
| Length | 1072 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1072 a.a. Molecular weight: 118689.40 Da Isoelectric Point: 6.4933
>WP_020323154.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNGQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFHVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGATEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKGRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHDDGQQAKSYGHEAGQQTGEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDYDKEGYGL
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNGQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFHVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGATEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKGRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHDDGQQAKSYGHEAGQQTGEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDYDKEGYGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0R8CGS3 |
T4CP
| ID | 3098 | GenBank | WP_020323171 |
| Name | t4cp2_PXH70_RS23610_2020CK-00216|unnamed2 |
UniProt ID | _ |
| Length | 510 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 510 a.a. Molecular weight: 58208.12 Da Isoelectric Point: 9.7037
>WP_020323171.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVSIIAGSVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGRDKRVILDPNGEYVETFYRENDIILNPYDKRSPGWSMFNEIRDEFDYDLYAVSIIQ
ESKERSAEEWYRYGRLLYREVSKKLLTHKRNPSMEEVLYWCNVAEYDKVKDFVKGTPAESLFIKGAERAT
GSARFVLGDKLASHLKMPEGNFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTFSQLVERYGINIAETLLGSMRSGIVMGSSRL
GKATLEHMSRALGEIEGEIERRQGSLLQSGPNNRPRIETRTVRAVTPTEISELRNLTGYIYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVSIIAGSVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGRDKRVILDPNGEYVETFYRENDIILNPYDKRSPGWSMFNEIRDEFDYDLYAVSIIQ
ESKERSAEEWYRYGRLLYREVSKKLLTHKRNPSMEEVLYWCNVAEYDKVKDFVKGTPAESLFIKGAERAT
GSARFVLGDKLASHLKMPEGNFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTFSQLVERYGINIAETLLGSMRSGIVMGSSRL
GKATLEHMSRALGEIEGEIERRQGSLLQSGPNNRPRIETRTVRAVTPTEISELRNLTGYIYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 54911..62187
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PXH70_RS23670 (PXH70_23670) | 49996..51021 | + | 1026 | WP_004199214 | IS21 family transposase | - |
| PXH70_RS23675 (PXH70_23675) | 51018..51797 | + | 780 | WP_004152394 | IS21-like element ISKpn7 family helper ATPase IstB | - |
| PXH70_RS23680 (PXH70_23680) | 52184..53065 | + | 882 | WP_004152396 | carbapenem-hydrolyzing class A beta-lactamase KPC-3 | - |
| PXH70_RS23685 (PXH70_23685) | 53315..54634 | - | 1320 | WP_004152397 | IS1182-like element ISKpn6 family transposase | - |
| PXH70_RS23690 (PXH70_23690) | 54911..55879 | - | 969 | WP_274850563 | type IV secretion system protein | virB6 |
| PXH70_RS23695 (PXH70_23695) | 55896..56138 | - | 243 | WP_000858958 | EexN family lipoprotein | - |
| PXH70_RS23700 (PXH70_23700) | 56148..56849 | - | 702 | WP_020323143 | type IV secretion system protein | virB5 |
| PXH70_RS23705 (PXH70_23705) | 56865..59237 | - | 2373 | WP_274850568 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| PXH70_RS23710 (PXH70_23710) | 59314..60282 | + | 969 | WP_013815099 | IS5-like element IS903B family transposase | - |
| PXH70_RS23715 (PXH70_23715) | 60328..60504 | - | 177 | WP_274850569 | hypothetical protein | virb4 |
| PXH70_RS23720 (PXH70_23720) | 60505..60822 | - | 318 | WP_000462629 | VirB3 family type IV secretion system protein | virB3 |
| PXH70_RS23725 (PXH70_23725) | 60862..61158 | - | 297 | WP_015060075 | TrbC/VirB2 family protein | virB2 |
| PXH70_RS23730 (PXH70_23730) | 61405..62187 | - | 783 | WP_032416587 | lytic transglycosylase domain-containing protein | virB1 |
| PXH70_RS23735 (PXH70_23735) | 62287..62366 | + | 80 | Protein_68 | KorB | - |
| PXH70_RS23740 (PXH70_23740) | 62407..62604 | + | 198 | WP_230201455 | H-NS family nucleoid-associated regulatory protein | - |
| PXH70_RS23745 (PXH70_23745) | 62608..62958 | + | 351 | WP_032416642 | hypothetical protein | - |
| PXH70_RS23750 (PXH70_23750) | 62955..63293 | + | 339 | WP_223226685 | hypothetical protein | - |
| PXH70_RS23755 (PXH70_23755) | 63297..63605 | + | 309 | WP_032416643 | hypothetical protein | - |
| PXH70_RS23760 (PXH70_23760) | 63890..64075 | + | 186 | WP_006118530 | ribbon-helix-helix domain-containing protein | - |
| PXH70_RS23765 (PXH70_23765) | 64072..64680 | - | 609 | WP_032449362 | recombinase family protein | - |
| PXH70_RS23770 (PXH70_23770) | 64846..65178 | - | 333 | WP_020323168 | hypothetical protein | - |
Host bacterium
| ID | 4788 | GenBank | NZ_CP118562 |
| Plasmid name | 2020CK-00216|unnamed2 | Incompatibility group | IncN3 |
| Plasmid size | 65317 bp | Coordinate of oriT [Strand] | 32944..33043 [-] |
| Host baterium | Enterobacter kobei strain 2020CK-00216 |
Cargo genes
| Drug resistance gene | blaKPC-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |