Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104341
Name   oriT_2020CK-00223|unnamed2 in_silico
Organism   Klebsiella pneumoniae strain 2020CK-00223
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP118400 (17168..17273 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_2020CK-00223|unnamed2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3178 GenBank   WP_004187323
Name   Relaxase_PWK27_RS27215_2020CK-00223|unnamed2 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   3092 GenBank   WP_004187443
Name   t4cp2_PWK27_RS27340_2020CK-00223|unnamed2 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79519.87 Da        Isoelectric Point: 4.8309

>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20069..48799

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWK27_RS27195 (PWK27_27195) 15153..15464 + 312 WP_004187333 hypothetical protein -
PWK27_RS27200 (PWK27_27200) 15597..16133 + 537 WP_004187332 hypothetical protein -
PWK27_RS27205 (PWK27_27205) 16635..17030 - 396 WP_019725163 hypothetical protein -
PWK27_RS27210 (PWK27_27210) 17065..17592 + 528 WP_172740549 plasmid mobilization protein MobA -
PWK27_RS27215 (PWK27_27215) 17579..19558 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
PWK27_RS27220 (PWK27_27220) 19572..20072 + 501 WP_004187320 DotD/TraH family lipoprotein -
PWK27_RS27225 (PWK27_27225) 20069..20848 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
PWK27_RS27230 (PWK27_27230) 20859..22022 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
PWK27_RS27235 (PWK27_27235) 22012..22272 + 261 WP_004187310 IcmT/TraK family protein traK
PWK27_RS27240 (PWK27_27240) 22297..25734 + 3438 WP_225373171 LPD7 domain-containing protein -
PWK27_RS27245 (PWK27_27245) 25700..26212 + 513 WP_011091071 hypothetical protein traL
PWK27_RS27250 (PWK27_27250) 26213..26425 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
PWK27_RS27255 (PWK27_27255) 26397..26807 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
PWK27_RS27260 (PWK27_27260) 26875..27588 + 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
PWK27_RS27265 (PWK27_27265) 27597..28748 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
PWK27_RS27270 (PWK27_27270) 28760..30109 + 1350 WP_004187474 conjugal transfer protein TraO traO
PWK27_RS27275 (PWK27_27275) 30121..30825 + 705 WP_015060002 conjugal transfer protein TraP traP
PWK27_RS27280 (PWK27_27280) 30849..31379 + 531 WP_004187478 conjugal transfer protein TraQ traQ
PWK27_RS27285 (PWK27_27285) 31396..31785 + 390 WP_004187479 DUF6750 family protein traR
PWK27_RS27290 (PWK27_27290) 31831..32325 + 495 WP_004187480 hypothetical protein -
PWK27_RS27295 (PWK27_27295) 32322..35372 + 3051 WP_274759739 conjugal transfer protein traU
PWK27_RS27300 (PWK27_27300) 35369..36577 + 1209 WP_004187486 conjugal transfer protein TraW traW
PWK27_RS27305 (PWK27_27305) 36574..37224 + 651 WP_004187488 hypothetical protein -
PWK27_RS27310 (PWK27_27310) 37217..39397 + 2181 WP_004187492 DotA/TraY family protein traY
PWK27_RS27315 (PWK27_27315) 39400..40053 + 654 WP_004187494 hypothetical protein -
PWK27_RS27320 (PWK27_27320) 40128..40358 + 231 WP_011091085 IncL/M type plasmid replication protein RepC -
PWK27_RS27325 (PWK27_27325) 40656..41711 + 1056 WP_159174226 plasmid replication initiator RepA -
PWK27_RS27330 (PWK27_27330) 42935..43030 + 96 WP_004206884 DinQ-like type I toxin DqlB -
PWK27_RS27335 (PWK27_27335) 43236..44348 - 1113 WP_001300563 IS4-like element IS421 family transposase -
PWK27_RS27340 (PWK27_27340) 44431..46518 - 2088 WP_004187443 hypothetical protein -
PWK27_RS27345 (PWK27_27345) 46531..47481 - 951 WP_004187441 DsbC family protein trbB
PWK27_RS27350 (PWK27_27350) 47492..48799 - 1308 WP_015062796 hypothetical protein trbA
PWK27_RS27355 (PWK27_27355) 48799..49194 - 396 WP_004187436 lytic transglycosylase domain-containing protein -
PWK27_RS27360 (PWK27_27360) 49299..49682 - 384 WP_032451295 DUF1496 domain-containing protein -
PWK27_RS27365 (PWK27_27365) 49762..50415 + 654 WP_039592068 type II CAAX endopeptidase family protein -
PWK27_RS27370 (PWK27_27370) 50568..51002 + 435 WP_015062799 translesion error-prone DNA polymerase V autoproteolytic subunit -
PWK27_RS27375 (PWK27_27375) 50990..52255 + 1266 WP_040224115 translesion error-prone DNA polymerase V subunit UmuC -
PWK27_RS27380 (PWK27_27380) 52278..53126 - 849 WP_021561452 3'-5' exonuclease -
PWK27_RS27385 (PWK27_27385) 53129..53449 - 321 WP_021561451 hypothetical protein -

Host bacterium

ID   4780 GenBank   NZ_CP118400
Plasmid name   2020CK-00223|unnamed2 Incompatibility group   IncL/M
Plasmid size   69147 bp Coordinate of oriT [Strand]   17168..17273 [+]
Host baterium   Klebsiella pneumoniae strain 2020CK-00223

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -