Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104331
• Name: oriT_KI4_B1|p1unnamed
• Organism: Fructilactobacillus cliffordii strain KI4_B1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP097120 (32960..32999 [-], 40 nt)
• oriT length: 40 nt
• IRs (inverted repeats): 18..23, 28..33 (AGGGAA..TTCCCT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 40 nt

>oriT_KI4_B1|p1unnamed
CAACTTCACGAGGAACGAGGGAACTGTTTCCCTTATGCTC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_M3M40_RS07240_KI4_B1|p1unnamed

• ID: 3171
• GenBank: WP_252767520
• Name: mobP2_M3M40_RS07240_KI4_B1|p1unnamed
• UniProt ID: _
• Length: 664 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 664 a.a.
• Molecular weight: 77649.04 Da
• Isoelectric Point: 9.8833

>WP_252767520.1 MobP2 family relaxase [Fructilactobacillus cliffordii]
MKIKPIEKNAANVIFTTQFKTANQMTGAGKSFTKFVDYTDRDEAVDLGLKKDPKYKDFGGFIEYMDRDAA
TNIDVAKTRKLTSNFNEFKDHLNQDEVKELKDDLTKAQANNSNIYSNVVSFSTKFLKENKIYDDDKNELN
QEALRDIIRESMTDYISSTPLKNPIWWGDIHVNTEHVHVHVGMAEINPTTPEVVVGDHKEHKGKLPEHCF
RVFKKQVINKTLEFGKNKELDHKYSLEKDIGIQKQVLIDRVSDYDDLNNLMLALPENRRLWRYSSNAEDM
KPAKKILDRMVNQFKDGNPAYKMWLLKTEQLLTIESKNYGQSDSLNKRKQDMDKRLANRILKECRQVQDK
ELEISVSNIAQDFSDNSIQDNQNIIKVIRNRLKDRDNIEISGSKINDLKHEISIRKIALKKQIASKKIEE
IKEKDSKLNQISDYGLKPKQKAVINKLTTFIHQENLEKIELHELRQQKPWNLNDNEKRKLDKLSNKYVNA
ENVQIDTVNKDMVNNSRKRLQDINKLSDLNIIETKSFQILADEYPDIRIRSASRDLSILELKLKIKNNSS
NGRDNKENYERLKALSTSTNNANHKYKHKDLLVKPSYNKKNYKTFKKPKLFNQKLSHSLNNLTNDLNKNL
DSSIQNLSKQDNKEKRLQKDMLNDQRRDDDEYSR

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (11-385)

Protein structure

No available structure.

T4CP

t4cp2_M3M40_RS07180_KI4_B1|p1unnamed

• ID: 3082
• GenBank: WP_252767545
• Name: t4cp2_M3M40_RS07180_KI4_B1|p1unnamed
• UniProt ID: _
• Length: 966 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 966 a.a.
• Molecular weight: 110724.21 Da
• Isoelectric Point: 9.4979

>WP_252767545.1 VirD4-like conjugal transfer protein, CD1115 family [Fructilactobacillus cliffordii]
MFGKSLGKGKNRRTQAQGPWWSQSKKLLIRLLLITILVAITLVGYMTQVNYELFKYLRNLKLTSLLDPES
KVNHNPVTLYDIFNLKGFWIFNDLGYSGSIMIFWIVFVSIGLSAVWYRIFRSYITYNSYEYGNESFENNS
LVVKQYTNIPDRNKSFPGYGGIPVLHKNKFWGKLMQIYTYILAPKIKQSNENLDKVPGIPGINIQKRVQY
SFQKIKFLGKYMIDQATNNTLIYGITRSGKGETFVFPLIDILSRAMKKVSIFVNDPKGELARSSYFTLIR
RGYKVVIIDLSNMYKSASYNPLQVIIDYARKGYTDQVQMEVNKISTSIYQTKQSGNSDPFWANSSSNLLN
ALILAQIDLARRNNDWSKVTFFNVYQMLTQMAGKEVVVDSDERITDDVQNGTKKSMLTHYFNLMAQAPHD
PYRQMAMDAFSQSSFAGSETQGSIFSSMMEGIKIYQQQDVAKLTSKNTVDFKESAFPRRFSLKLMDTLQL
KTANVTFRDASGHLIESRKQDIDNLGYLNYALKAKLPRNFEITINVENHVYVFTGSIVRKQKVFGNPFKA
DVKVARQNIRILKNKKLKFIETKSTDNNYSDVQLKYSEQPVAVFFVTPPNNDAYNQIVSFAIDQSFNQMY
EMATQNGGKCFRRVHYVLDEFGNLPTVNSMNTKISIGLGQEILFDIVVQNDEQLSDKYDDKVANTIKANC
SNTLYILSNDDKTTELISKKLGKTTVMTRNHSITPDLNIGRDTLNTNQTAQDIMSSVELQHLMVGQQVLL
RANTRQSIYGNKIRNNPIFANGLKYEMPSRWQFLQDTFDTSTIMSDIPLKCIHSKMSLKDNAYNYFNNGM
NVSDNTELDKMNNLAGLSNSSTKISSSEKEIIKQVINEDDIRNAQEIENTYVDPLFTDEEISDESFLNKQ
LPKILNISKKQSSEGEEINEFLNSKTAIERINFIKNHNDQDFWNIFGITRENLDLD

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (221-473)

• TraM recognition site of TraD and TraG (TraG-D_C) (643-763)

Protein structure

No available structure.

Host bacterium

• ID: 4770
• GenBank: NZ_CP097120
• Plasmid name: KI4_B1|p1unnamed
• Incompatibility group: -
• Plasmid size: 41034 bp
• Coordinate of oriT [Strand]: 32960..32999 [-]
• Host baterium: Fructilactobacillus cliffordii strain KI4_B1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -