Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104329
Name   oriT_pUA1136 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MW655523 (48413..48501 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pUA1136
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3170 GenBank   WP_156004660
Name   nikB_PYI35_RS00305_pUA1136 insolico UniProt ID   _
Length   900 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 900 a.a.        Molecular weight: 104107.69 Da        Isoelectric Point: 7.5153

>WP_156004660.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPMR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   1386 GenBank   WP_001283947
Name   WP_001283947_pUA1136 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   3080 GenBank   WP_125340118
Name   trbC_PYI35_RS00315_pUA1136 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86988.16 Da        Isoelectric Point: 6.7713

>WP_125340118.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVKAERAGINLLTFPQDNNHPTERPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54859..94109

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PYI35_RS00310 (pUA1136_00067) 51684..52502 + 819 WP_062865724 DUF2971 domain-containing protein -
PYI35_RS00315 (pUA1136_00068) 52575..54866 - 2292 WP_125340118 F-type conjugative transfer protein TrbC -
PYI35_RS00320 (pUA1136_00069) 54859..55929 - 1071 WP_274703475 IncI1-type conjugal transfer protein TrbB trbB
PYI35_RS00325 (pUA1136_00070) 55948..57156 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
PYI35_RS00330 (pUA1136_00071) 57372..58358 - 987 WP_342455467 hypothetical protein -
PYI35_RS00335 58928..59080 + 153 WP_001303307 Hok/Gef family protein -
PYI35_RS00340 (pUA1136_00072) 59152..59403 - 252 WP_001291965 hypothetical protein -
PYI35_RS00345 59904..59999 + 96 WP_000609148 DinQ-like type I toxin DqlB -
PYI35_RS00350 60064..60348 - 285 WP_233109162 hypothetical protein -
PYI35_RS00355 (pUA1136_00075) 60449..60658 - 210 WP_000416064 hemolysin expression modulator Hha -
PYI35_RS00360 (pUA1136_00076) 60756..61370 - 615 WP_001749187 plasmid IncI1-type surface exclusion protein ExcA -
PYI35_RS00365 (pUA1136_00077) 61446..63614 - 2169 WP_031942447 IncI1-type conjugal transfer membrane protein TraY traY
PYI35_RS00370 (pUA1136_00078) 63711..64295 - 585 WP_001037982 IncI1-type conjugal transfer protein TraX -
PYI35_RS00375 (pUA1136_00079) 64324..65526 - 1203 WP_274703477 IncI1-type conjugal transfer protein TraW traW
PYI35_RS00380 (pUA1136_00080) 65493..66107 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
PYI35_RS00385 (pUA1136_00081) 66107..69151 - 3045 WP_274703478 IncI1-type conjugal transfer protein TraU traU
PYI35_RS00390 (pUA1136_00082) 69241..70041 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
PYI35_RS00395 (pUA1136_00083) 70025..70213 - 189 WP_001277255 putative conjugal transfer protein TraS -
PYI35_RS00400 (pUA1136_00084) 70277..70681 - 405 WP_000086959 IncI1-type conjugal transfer protein TraR traR
PYI35_RS00405 (pUA1136_00085) 70732..71259 - 528 WP_001055569 conjugal transfer protein TraQ traQ
PYI35_RS00410 (pUA1136_00086) 71259..71963 - 705 WP_000801919 IncI1-type conjugal transfer protein TraP traP
PYI35_RS00415 (pUA1136_00087) 71963..73252 - 1290 WP_274703479 conjugal transfer protein TraO traO
PYI35_RS00420 (pUA1136_00088) 73255..74238 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
PYI35_RS00425 (pUA1136_00089) 74249..74941 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
PYI35_RS00430 (pUA1136_00090) 74938..75285 - 348 WP_001055900 conjugal transfer protein traL
PYI35_RS00435 (pUA1136_00091) 75303..79070 - 3768 WP_274703489 LPD7 domain-containing protein -
PYI35_RS00440 (pUA1136_00092) 79160..79711 - 552 WP_000014583 phospholipase D family protein -
PYI35_RS00445 (pUA1136_00093) 79726..80016 - 291 WP_001299214 hypothetical protein traK
PYI35_RS00450 (pUA1136_00094) 80013..81161 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
PYI35_RS00455 (pUA1136_00095) 81158..81976 - 819 WP_000646095 IncI1-type conjugal transfer lipoprotein TraI traI
PYI35_RS00460 (pUA1136_00096) 81973..82431 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
PYI35_RS00465 (pUA1136_00097) 82826..83410 - 585 WP_000977522 histidine phosphatase family protein -
PYI35_RS00470 (pUA1136_00098) 83470..84672 - 1203 WP_000976353 conjugal transfer protein TraF -
PYI35_RS00475 (pUA1136_00099) 84758..85582 - 825 WP_001545755 conjugal transfer protein TraE traE
PYI35_RS00480 (pUA1136_00100) 85733..86887 - 1155 WP_001139958 site-specific integrase -
PYI35_RS00580 86928..87161 + 234 Protein_96 prepilin -
PYI35_RS00485 (pUA1136_00101) 88282..89616 - 1335 WP_112413233 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
PYI35_RS00490 (pUA1136_00102) 89616..90272 - 657 WP_001193549 A24 family peptidase -
PYI35_RS00495 (pUA1136_00103) 90257..90817 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
PYI35_RS00500 (pUA1136_00104) 90827..91441 - 615 WP_000908227 type 4 pilus major pilin -
PYI35_RS00505 (pUA1136_00105) 91458..92543 - 1086 WP_001208802 type II secretion system F family protein -
PYI35_RS00510 (pUA1136_00106) 92556..94109 - 1554 WP_001545756 ATPase, T2SS/T4P/T4SS family virB11
PYI35_RS00515 (pUA1136_00107) 94120..94572 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
PYI35_RS00520 (pUA1136_00108) 94559..95854 - 1296 WP_001545757 type 4b pilus protein PilO2 -
PYI35_RS00525 (pUA1136_00109) 95847..97529 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
PYI35_RS00530 (pUA1136_00110) 97543..97980 - 438 WP_001545758 type IV pilus biogenesis protein PilM -
PYI35_RS00535 (pUA1136_00111) 97980..99047 - 1068 WP_016245573 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   4768 GenBank   NZ_MW655523
Plasmid name   pUA1136 Incompatibility group   IncI1
Plasmid size   104489 bp Coordinate of oriT [Strand]   48413..48501 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028

Cargo genes

Drug resistance gene   blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -