Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104326
Name   oriT_pUA1135 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MW590592 (40897..40985 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pUA1135
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3167 GenBank   WP_117248730
Name   nikB_PYI32_RS00285_pUA1135 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103941.36 Da        Isoelectric Point: 7.2602

>WP_117248730.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMDDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   1384 GenBank   WP_001283947
Name   WP_001283947_pUA1135 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   3078 GenBank   WP_001289276
Name   trbC_PYI32_RS00290_pUA1135 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46363..86159

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PYI32_RS00290 (pUA1135_00057) 44079..46370 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
PYI32_RS00295 (pUA1135_00058) 46363..47433 - 1071 WP_000151582 IncI1-type conjugal transfer protein TrbB trbB
PYI32_RS00300 (pUA1135_00059) 47452..48660 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
PYI32_RS00305 (pUA1135_00060) 48967..49746 - 780 WP_275450201 protein FinQ -
PYI32_RS00310 (pUA1135_00061) 50373..50525 + 153 WP_001303307 Hok/Gef family protein -
PYI32_RS00315 (pUA1135_00062) 50597..50848 - 252 WP_001291968 hypothetical protein -
PYI32_RS00320 50891..51055 + 165 WP_001323923 hypothetical protein -
PYI32_RS00325 51772..52056 - 285 WP_014640317 hypothetical protein -
PYI32_RS00330 (pUA1135_00064) 52157..52366 - 210 WP_001140545 hemolysin expression modulator Hha -
PYI32_RS00335 (pUA1135_00065) 52464..53078 - 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
PYI32_RS00340 (pUA1135_00066) 53154..55322 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
PYI32_RS00345 (pUA1135_00067) 55419..56003 - 585 WP_001579774 IncI1-type conjugal transfer protein TraX -
PYI32_RS00350 (pUA1135_00068) 56032..57234 - 1203 WP_001579773 IncI1-type conjugal transfer protein TraW traW
PYI32_RS00355 (pUA1135_00069) 57201..57815 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
PYI32_RS00360 (pUA1135_00070) 57815..60859 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
PYI32_RS00365 (pUA1135_00071) 60949..61749 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
PYI32_RS00370 (pUA1135_00072) 61733..61921 - 189 WP_001277255 putative conjugal transfer protein TraS -
PYI32_RS00375 (pUA1135_00073) 61985..62389 - 405 WP_001579770 DUF6750 family protein traR
PYI32_RS00380 (pUA1135_00074) 62440..62967 - 528 WP_001055569 conjugal transfer protein TraQ traQ
PYI32_RS00385 (pUA1135_00075) 62967..63671 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
PYI32_RS00390 (pUA1135_00076) 63671..64960 - 1290 WP_001271997 conjugal transfer protein TraO traO
PYI32_RS00395 (pUA1135_00077) 64963..65946 - 984 WP_001579769 IncI1-type conjugal transfer protein TraN traN
PYI32_RS00400 (pUA1135_00078) 65957..66649 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
PYI32_RS00405 (pUA1135_00079) 66646..66993 - 348 WP_001055900 conjugal transfer protein traL
PYI32_RS00410 (pUA1135_00080) 67011..70778 - 3768 WP_001579768 LPD7 domain-containing protein -
PYI32_RS00415 (pUA1135_00081) 70868..71419 - 552 WP_000014584 phospholipase D family protein -
PYI32_RS00420 (pUA1135_00082) 71434..71724 - 291 WP_001372180 hypothetical protein traK
PYI32_RS00425 (pUA1135_00083) 71721..72869 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
PYI32_RS00430 (pUA1135_00084) 72866..73684 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
PYI32_RS00435 (pUA1135_00085) 73681..74139 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
PYI32_RS00440 (pUA1135_00086) 74534..75118 - 585 WP_001579766 histidine phosphatase family protein -
PYI32_RS00445 (pUA1135_00087) 75178..76380 - 1203 WP_000976353 conjugal transfer protein TraF -
PYI32_RS00450 (pUA1135_00088) 76466..77290 - 825 WP_001238929 conjugal transfer protein TraE traE
PYI32_RS00455 (pUA1135_00089) 77441..78595 - 1155 WP_001139958 site-specific integrase -
PYI32_RS00460 78579..79001 + 423 WP_162847923 prepilin, shufflon protein A -
PYI32_RS00560 78998..79222 - 225 Protein_92 hypothetical protein -
PYI32_RS00465 79490..79645 - 156 WP_001302705 hypothetical protein -
PYI32_RS00470 (pUA1135_00091) 80283..81653 - 1371 WP_001393542 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
PYI32_RS00475 (pUA1135_00092) 81653..82309 - 657 WP_001193553 prepilin peptidase -
PYI32_RS00480 (pUA1135_00093) 82294..82854 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
PYI32_RS00485 (pUA1135_00094) 82864..83478 - 615 WP_000959786 type 4 pilus major pilin -
PYI32_RS00490 (pUA1135_00095) 83496..84593 - 1098 WP_001208805 type II secretion system F family protein -
PYI32_RS00495 (pUA1135_00096) 84606..86159 - 1554 WP_001579763 ATPase, T2SS/T4P/T4SS family virB11
PYI32_RS00500 (pUA1135_00097) 86170..86622 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
PYI32_RS00505 (pUA1135_00098) 86609..87904 - 1296 WP_000752772 type 4b pilus protein PilO2 -
PYI32_RS00510 (pUA1135_00099) 87897..89579 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
PYI32_RS00515 (pUA1135_00100) 89593..90030 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
PYI32_RS00520 (pUA1135_00101) 90030..91097 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   4765 GenBank   NZ_MW590592
Plasmid name   pUA1135 Incompatibility group   IncI1
Plasmid size   96685 bp Coordinate of oriT [Strand]   40897..40985 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028

Cargo genes

Drug resistance gene   blaCTX-M-14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2