Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104325 |
| Name | oriT_pUA1141  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MW655528 (14062..14110 [+], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_pUA1141
CGATTGTAACATACCCGGAACGGGTTTGTGTACAATCGGTCTCGTGCCT
CGATTGTAACATACCCGGAACGGGTTTGTGTACAATCGGTCTCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3077 | GenBank | WP_274703342 |
| Name | t4cp2_PYH81_RS00220_pUA1141 |
UniProt ID | _ |
| Length | 655 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 655 a.a. Molecular weight: 74042.97 Da Isoelectric Point: 8.2340
>WP_274703342.1 type IV secretory system conjugative DNA transfer family protein [Salmonella enterica]
MKKKIILVVFLLLILVACLIGGNYLGGYAALKYSGLSIDMLKWNTFNNVITQFSGKPEYKKLVAITWAGF
AAPCAIFIGFVVIVIVGLMPKKVIYGNARLATDMDLAKSTFFPTDKELKEARLKNSKPYCFPPILIGKQF
KGRFKNKYIYFFGQQFLILYAPTRSGKGVGIVIPNCVNYPDSMVVLDIKLENWFLSAGYRQNVLGQECFL
FAPAGYAENQQEAKKGNIRSHRWNPLDCVNRSDIQRSGDLEKIAAMLIPASDDPIWSDSARKLFCGLALY
LLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQKEYLSDETKSLFREFMAAP
EKTQGSIITNFSSPLSIFKNPITAAATDASDFDVRMVRKKPMSIYLGLTPDALITHSRLVNLFFSMLVNE
NTKELPEQNPELKYQCLVLLDEFTSMGKSEIIEKGVGFTAGFNLRFVIILQNEGQGKKDDMYGTHGWETF
VENSAVVLYYPPKAKNELAKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSRNHEIVSRAVLLPEEIVELR
DYKNKMGNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMPVLFDEEMRNQILEQAKIYS
DDISMFSDVMTKPDLETHDLEDVSE
MKKKIILVVFLLLILVACLIGGNYLGGYAALKYSGLSIDMLKWNTFNNVITQFSGKPEYKKLVAITWAGF
AAPCAIFIGFVVIVIVGLMPKKVIYGNARLATDMDLAKSTFFPTDKELKEARLKNSKPYCFPPILIGKQF
KGRFKNKYIYFFGQQFLILYAPTRSGKGVGIVIPNCVNYPDSMVVLDIKLENWFLSAGYRQNVLGQECFL
FAPAGYAENQQEAKKGNIRSHRWNPLDCVNRSDIQRSGDLEKIAAMLIPASDDPIWSDSARKLFCGLALY
LLDKERFHLQQKKKGVADVPDVLVSMSAIMKLSVPESGQKLSAWMGTEIDQKEYLSDETKSLFREFMAAP
EKTQGSIITNFSSPLSIFKNPITAAATDASDFDVRMVRKKPMSIYLGLTPDALITHSRLVNLFFSMLVNE
NTKELPEQNPELKYQCLVLLDEFTSMGKSEIIEKGVGFTAGFNLRFVIILQNEGQGKKDDMYGTHGWETF
VENSAVVLYYPPKAKNELAKKISEEIGVRDMKIIKKSDSRSGGKGGSSRSRNHEIVSRAVLLPEEIVELR
DYKNKMGNMAVREIVMSEYTRPFVANKIIWFEEEEFKKRVDIAKENPVEMPVLFDEEMRNQILEQAKIYS
DDISMFSDVMTKPDLETHDLEDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 25344..34117
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PYH81_RS00145 (pUA1141_00029) | 21163..21966 | + | 804 | WP_000210482 | replication initiation protein | - |
| PYH81_RS00150 (pUA1141_00030) | 23592..23930 | - | 339 | WP_000612443 | helix-turn-helix transcriptional regulator | - |
| PYH81_RS00155 (pUA1141_00031) | 24127..24414 | + | 288 | WP_000332248 | helix-turn-helix transcriptional regulator | - |
| PYH81_RS00160 (pUA1141_00032) | 24455..24682 | - | 228 | WP_032279333 | EexN family lipoprotein | - |
| PYH81_RS00165 (pUA1141_00033) | 24701..25342 | - | 642 | WP_264111655 | type IV secretion system protein | - |
| PYH81_RS00170 (pUA1141_00034) | 25344..26339 | - | 996 | WP_001103651 | type IV secretion system protein | virB6 |
| PYH81_RS00175 (pUA1141_00035) | 26524..26709 | + | 186 | WP_126677389 | hypothetical protein | - |
| PYH81_RS00180 (pUA1141_00036) | 26699..27283 | + | 585 | WP_250697594 | lytic transglycosylase domain-containing protein | virB1 |
| PYH81_RS00185 (pUA1141_00037) | 27276..27569 | + | 294 | WP_001031683 | TrbC/VirB2 family protein | virB2 |
| PYH81_RS00190 (pUA1141_00038) | 27627..27947 | + | 321 | WP_001000142 | VirB3 family type IV secretion system protein | virB3 |
| PYH81_RS00195 (pUA1141_00039) | 27950..30322 | + | 2373 | WP_001195522 | VirB4 family type IV secretion system protein | virb4 |
| PYH81_RS00200 (pUA1141_00041) | 30453..31190 | + | 738 | WP_000364523 | type IV secretion system protein | virB8 |
| PYH81_RS00205 (pUA1141_00042) | 31187..31966 | + | 780 | WP_000592647 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| PYH81_RS00210 (pUA1141_00043) | 31956..33098 | + | 1143 | WP_274703341 | TrbI/VirB10 family protein | virB10 |
| PYH81_RS00215 (pUA1141_00044) | 33098..34117 | + | 1020 | WP_001287982 | P-type DNA transfer ATPase VirB11 | virB11 |
| PYH81_RS00220 (pUA1141_00045) | 34168..36135 | + | 1968 | WP_274703342 | type IV secretory system conjugative DNA transfer family protein | - |
| PYH81_RS00225 (pUA1141_00046) | 36151..36456 | + | 306 | WP_001223992 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| PYH81_RS00230 (pUA1141_00047) | 36557..37729 | + | 1173 | WP_274703343 | YadA-like family protein | - |
| PYH81_RS00235 (pUA1141_00048) | 37759..37986 | + | 228 | WP_274703344 | hypothetical protein | - |
| PYH81_RS00240 (pUA1141_00049) | 38111..38698 | + | 588 | WP_274703345 | DUF6012 family protein | - |
Host bacterium
| ID | 4764 | GenBank | NZ_MW655528 |
| Plasmid name | pUA1141 | Incompatibility group | - |
| Plasmid size | 38709 bp | Coordinate of oriT [Strand] | 14062..14110 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |