Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104319
Name   oriT_p1.15-2E5 in_silico
Organism   Salmonella enterica subsp. enterica serovar Enteritidis strain 1.15-2E5
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MN125609 (79724..79993 [+], 270 nt)
oriT length   270 nt
IRs (inverted repeats)      245..253, 262..270  (CACCCCTGG..CCAGGGGTG)
 92..98, 111..117  (ATTTAAT..ATTAAAT)
 31..38, 41..48  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 270 nt

>oriT_p1.15-2E5
GTCCTCACCACCAAAAGCACCACACCCCACGCAAAATTAAAATTTTGCTGATTTGTTTTTAGTAACAGTGAGTTATGTTCATAATAATGTAATTTAATTTATTTTAAAGTATTAAATAGCGGTGCAGGCACGTCGCCGGCACCGCTATGACGACGCTATACAGGGGTAGTACTGACTATTTTTATAAAAAACATTGCATTATATCAGGGGCGCTGCTAGCGTCGCTGTGCCTGTTTTTAAGTAACACCCCTGGGGGCGCTGCCAGGGGTG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3164 GenBank   WP_000539539
Name   Relaxase_H5D22_RS00390_p1.15-2E5 insolico UniProt ID   A0A748H4F7
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44419.77 Da        Isoelectric Point: 10.5368

>WP_000539539.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(70-227)


  Protein structure


Source ID Structure
AlphaFold DB A0A748H4F7


Host bacterium

ID   4758 GenBank   NZ_MN125609
Plasmid name   p1.15-2E5 Incompatibility group   IncFIB
Plasmid size   93619 bp Coordinate of oriT [Strand]   79724..79993 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Enteritidis strain 1.15-2E5

Cargo genes

Drug resistance gene   blaTEM-1B, sul2, aph(3'')-Ib, aph(6)-Id, tet(A)
Virulence gene   pefD, pefC, pefA, pefB, spvC, spvB, rck
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -