Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104313
Name   oriT_p11743_2 in_silico
Organism   Enterobacter kobei strain 11743-yvys
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP083864 (11963..12052 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_p11743_2
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3160 GenBank   WP_024561907
Name   mobF_LCD49_RS26565_p11743_2 insolico UniProt ID   H1ZMV6
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118896.59 Da        Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure


Source ID Structure
AlphaFold DB H1ZMV6


T4CP

ID   3075 GenBank   WP_000139145
Name   t4cp2_LCD49_RS26560_p11743_2 insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57854.92 Da        Isoelectric Point: 9.5852

>WP_000139145.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERGHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 42444..52732

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD49_RS26655 (LCD49_26720) 37932..38792 + 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -
LCD49_RS26660 (LCD49_26725) 39051..39527 + 477 WP_013023839 WbuC family cupin fold metalloprotein -
LCD49_RS26665 (LCD49_26730) 39574..40449 - 876 WP_011091028 extended-spectrum class A beta-lactamase CTX-M-3 -
LCD49_RS26670 (LCD49_26735) 40784..42046 - 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
LCD49_RS26675 (LCD49_26740) 42444..43445 - 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
LCD49_RS26680 (LCD49_26745) 43496..44629 - 1134 WP_000101920 type IV secretion system protein VirB10 virB10
LCD49_RS26685 (LCD49_26750) 44629..45516 - 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
LCD49_RS26690 (LCD49_26755) 45527..46222 - 696 WP_001208352 type IV secretion system protein virB8
LCD49_RS26955 46233..46364 - 132 WP_032493177 hypothetical protein -
LCD49_RS26695 (LCD49_26760) 46441..47478 - 1038 WP_101972614 type IV secretion system protein virB6
LCD49_RS26700 (LCD49_26765) 47495..47737 - 243 WP_000858958 EexN family lipoprotein -
LCD49_RS26705 (LCD49_26770) 47747..48460 - 714 WP_000644929 type IV secretion system protein virB5
LCD49_RS26710 (LCD49_26775) 48476..51049 - 2574 WP_032493176 VirB4 family type IV secretion/conjugal transfer ATPase virb4
LCD49_RS26715 (LCD49_26780) 51050..51367 - 318 WP_000462629 VirB3 family type IV secretion system protein virB3
LCD49_RS26720 (LCD49_26785) 51407..51703 - 297 WP_015060075 TrbC/VirB2 family protein virB2
LCD49_RS26725 (LCD49_26790) 51950..52732 - 783 WP_001642193 lytic transglycosylase domain-containing protein virB1
LCD49_RS26730 (LCD49_26795) 52832..53149 + 318 WP_000960955 H-NS family nucleoid-associated regulatory protein -
LCD49_RS26735 (LCD49_26800) 53153..53503 + 351 WP_000528713 hypothetical protein -
LCD49_RS26740 (LCD49_26805) 53500..53838 + 339 WP_000040982 hypothetical protein -
LCD49_RS26745 (LCD49_26810) 53842..54150 + 309 WP_000733764 hypothetical protein -
LCD49_RS26750 (LCD49_26815) 54435..54620 + 186 WP_024562121 ribbon-helix-helix domain-containing protein -
LCD49_RS26755 (LCD49_26820) 54617..55225 - 609 WP_000275218 recombinase family protein -
LCD49_RS26760 (LCD49_26825) 55391..55723 - 333 WP_000140165 hypothetical protein -

Host bacterium

ID   4752 GenBank   NZ_CP083864
Plasmid name   p11743_2 Incompatibility group   IncN2
Plasmid size   55862 bp Coordinate of oriT [Strand]   11963..12052 [-]
Host baterium   Enterobacter kobei strain 11743-yvys

Cargo genes

Drug resistance gene   qnrS2, blaTEM-1B, blaCTX-M-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -