Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104297
Name   oriT_MesoLjLa2 in_silico
Organism   Mesorhizobium sp. L-2-11
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP023258 (61831..61877 [-], 47 nt)
oriT length   47 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 47 nt

>oriT_MesoLjLa2
GGATCCAAGGGCGCAATTATACGTCGCTGATACGACGCGTTGCGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3152 GenBank   WP_202367774
Name   traA_JG739_RS31830_MesoLjLa2 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123106.87 Da        Isoelectric Point: 10.1771

>WP_202367774.1 Ti-type conjugative transfer relaxase TraA [Mesorhizobium sp. L-2-11]
MAIAHFSVSIVSRGDGRSAVLSAAYRHCAKMDYEREARTVDYSRKQGLVHEEFLLPADAPQWARKLIADR
SVTGAAEAFWNKVEAFEKRSDAQLAKDLTIALPLELSAEQNIALVRDFVEKHILSKGMVADWVYHDNLGN
PHIHLMMTLRPLTEDGFGAKKVAVLGEGGELLRTKAGKIVYELWAGDTLDFNAFRDGWFERLNHHLALNG
VNLRIDGRSYEKQGIELVPTIHVGVGAKAIQRKATAEGWKPSLERLDLQEERKAENLRRIQARPEIVLDL
VTREKSVFDIRDIAKVLHRYVDDPAAFQQLLARIIQSPEVLRLQRDTMAFATGERVPARYTTRELIRLEA
EMARRSVWLSGRETHGVPEKVLEATFARHARLSGEQRTAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGAALAGKAAEGLEKEAGIASRTLASWELRWKQGRDTLDAKTVFVMDEAGMVASKQMTGFVE
AVVRAGAKLVLVGDPEQLQPIEAGAAFRAIVDRIGYADLETIYRQREDWMRKASLDLARGHVDQALAAYR
SQGRVLGSELKAQAVENLIADWNRDYDPAKTTLILAHLRRDVRLLNNMAREKLVERGILSEGHGFRTADG
IRHFDAGDQIVFLKNEGSLGVKNGMIGRVVEAAPNRITVSLGEGDQRRQVTVEQRFYNNLDHGYATTIHK
AQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGRRSFAFNGGLTKILSRRRPKETTLDYERGTLY
RRALAFAENRGLHIVHVARTMLRDRLDWTLRQSSKLADLAIRLRTAGERLGLLQVPITRTKKEARPMVAG
VKLFPKSLGDAVEQKLADDPALKKQWEEVSTRFRYVFADPETAFRTMNFDAVLADGRAASQTLQNLTINP
ASIGPLNGKTGLLASKADREARRVADVNVPALKRDIERYLEVRERALQRIEGEEKTLRQRVSIDIPALSP
AALSFLERVRDAIDRNDLPAALGYALSNREAKLEIDGFNKAVAERFGERTLLTNAARNPTGQLFDKLAGG
LQPQEKERLKEAWPVMRTAQQLAAHERTTAMLKQAEDLALSQRQTPVMKQ

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-950)

AAA domain (AAA_30)

(382-565)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(691-736)

MobA/MobL family (MobA_MobL)

(17-243)


  Protein structure



No available structure.




T4CP

ID   3067 GenBank   WP_202367758
Name   t4cp2_JG739_RS31710_MesoLjLa2 insolico UniProt ID   _
Length   822 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 822 a.a.        Molecular weight: 91553.42 Da        Isoelectric Point: 5.8131

>WP_202367758.1 conjugal transfer protein TrbE [Mesorhizobium sp. L-2-11]
MVALKTFRQSGPSFADLVPYAGLVDNGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINTILARLG
SGWMIQVEAVRVPTSDYPDEADCHFPDPVTRAIDFERRLHFQREHGHFESRHALILTWRPPEPRRSGLAR
YVYSDPASRSASYAVTTLDTFRASIREMEQYLGNVVSIQRMATREVDERAGFRVARYDELLQFVRFCITG
ENHPLRLPDIPMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNALDLMPLTYRWSSRFVF
LDEQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMMMVAETEDAIAEASSQLVAYGYYTPVIVL
FDGAQARLQEKCEAIRRLVQAEGFGARIETLNATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSVWS
GSPVAPCPFYPPGSPPLVQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYAGAQVFA
FDKGRSMLPLTLGLDGDHYEIGGDATGGGEGASPALAFCPLAELSSDGDRAWAAEWIETLVALQGVSVTP
DYRNAISRQIGLMAQSRGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEEDGLSLRAFQCFEVEGL
MNMGERNLVPVLTYLFRRIEKRLTGAPSLILLDEAWLMLGHPTFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREMGTREFYERIGFNERQIEIVATALPKREYYVVSPEGRRLFDM
ALGPVALSFVGASGKEDLKQIRALHSKHGAAWPLQWLQQRGIADVESFFPKA

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(191-400)

  Protein structure



No available structure.



ID   3068 GenBank   WP_202367777
Name   traG_JG739_RS31845_MesoLjLa2 insolico UniProt ID   _
Length   643 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 643 a.a.        Molecular weight: 69217.24 Da        Isoelectric Point: 9.6425

>WP_202367777.1 Ti-type conjugative transfer system protein TraG [Mesorhizobium sp. L-2-11]
MTANRTVLALIPATMMIGVTILIPGIEHWLAAFGKTAQAKLMLGRIGLALPYAMAAAIGVIFLFAANGAA
NIKAAGWGVVAGSAATILIAVAREAVRLAGIASNVPSGQSVFAYVDPATTLGASAAFFVGVFALRVAIKG
NAAFARPAPRRIHGRRAVHGEADWMGMTEAARMFPDAGGIVIGERYRVDRDSVMAVSFRADAQGTWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLIVLDPSSEVAPMVVDHRRRAGRKVIVLD
PASPATGFNALDWIGRFGATREEDIVAVATWIMTDNARAASARDDFFRASAMQLLTALIADVCLSGNTEE
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGDSFSTDELAVGGTDIFIALDMKVLEAHPGLARVVIGSFLNAIYNRNGEVAAKTLFLLDEVARLGY
LRILETARDAGRKYGISLTLIFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYLSKRCGDTT
VEVDQTNRSSGMRGSSRSRSKQLNRRPLILPHEVMRMRADEQIVFTAGNPPLRCGRAIWFRRDDMKACVG
TNRFHRSEGGDSG

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-635)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32961..42580

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG739_RS31680 (MesoLjLa_63430) 29517..30524 - 1008 WP_202367753 plasmid partitioning protein RepB -
JG739_RS31685 (MesoLjLa_63440) 30751..31968 - 1218 WP_202367754 plasmid partitioning protein RepA -
JG739_RS31690 (MesoLjLa_63450) 32329..32964 + 636 WP_202367911 acyl-homoserine-lactone synthase -
JG739_RS31695 (MesoLjLa_63460) 32961..33932 + 972 WP_202367755 P-type conjugative transfer ATPase TrbB virB11
JG739_RS31700 (MesoLjLa_63470) 33922..34299 + 378 WP_202367756 TrbC/VirB2 family protein virB2
JG739_RS31705 (MesoLjLa_63480) 34292..34591 + 300 WP_202367757 conjugal transfer protein TrbD virB3
JG739_RS31710 (MesoLjLa_63490) 34599..37067 + 2469 WP_202367758 conjugal transfer protein TrbE virb4
JG739_RS31715 (MesoLjLa_63500) 37039..37848 + 810 WP_202367759 P-type conjugative transfer protein TrbJ virB5
JG739_RS31720 (MesoLjLa_63510) 37845..38063 + 219 WP_202367760 entry exclusion protein TrbK -
JG739_RS31725 (MesoLjLa_63520) 38057..39256 + 1200 WP_202367761 P-type conjugative transfer protein TrbL virB6
JG739_RS31730 (MesoLjLa_63530) 39271..39933 + 663 WP_202367762 conjugal transfer protein TrbF virB8
JG739_RS31735 (MesoLjLa_63540) 40013..40801 + 789 WP_244750023 P-type conjugative transfer protein TrbG virB9
JG739_RS31740 (MesoLjLa_63550) 40801..41256 + 456 WP_202367764 conjugal transfer protein TrbH -
JG739_RS31745 (MesoLjLa_63560) 41267..42580 + 1314 WP_202367765 IncP-type conjugal transfer protein TrbI virB10
JG739_RS31750 (MesoLjLa_63570) 44148..44957 + 810 Protein_41 DNA repair exonuclease -
JG739_RS31755 45105..46844 + 1740 WP_202367767 histidinol-phosphate transaminase -
JG739_RS31760 (MesoLjLa_63600) 46986..47429 + 444 WP_244750011 transposase -

Host bacterium

ID   4736 GenBank   NZ_AP023258
Plasmid name   MesoLjLa2 Incompatibility group   -
Plasmid size   287750 bp Coordinate of oriT [Strand]   61831..61877 [-]
Host baterium   Mesorhizobium sp. L-2-11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -