Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104279 |
| Name | oriT_pHLJ179-85  |
| Organism | Escherichia fergusonii |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN232214 (13631..13683 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pHLJ179-85
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3048 | GenBank | WP_015059539 |
| Name | t4cp2_HTY27_RS00240_pHLJ179-85 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33615..57072
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTY27_RS00175 | 29104..30228 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTY27_RS00180 | 30277..30585 | + | 309 | WP_001199277 | hypothetical protein | - |
| HTY27_RS00185 | 31128..31283 | - | 156 | WP_001358489 | hypothetical protein | - |
| HTY27_RS00425 | 31359..31590 | + | 232 | Protein_40 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTY27_RS00190 | 31587..32963 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTY27_RS00195 | 32976..33611 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTY27_RS00200 | 33615..34097 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTY27_RS00205 | 34163..34720 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTY27_RS00210 | 34765..35874 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTY27_RS00215 | 35865..37403 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTY27_RS00220 | 37428..37922 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTY27_RS00225 | 37906..39228 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTY27_RS00230 | 39267..40910 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTY27_RS00235 | 40903..41439 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTY27_RS00240 | 41486..43444 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTY27_RS00245 | 43460..44515 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTY27_RS00250 | 44534..45673 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTY27_RS00255 | 45663..46364 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTY27_RS00260 | 46430..47164 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTY27_RS00265 | 47330..49687 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTY27_RS00270 | 49693..50013 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTY27_RS00410 | 50084..50374 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTY27_RS00280 | 50374..50958 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTY27_RS00285 | 50979..51377 | - | 399 | WP_001153665 | hypothetical protein | - |
| HTY27_RS00290 | 51496..51933 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTY27_RS00295 | 51939..53174 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTY27_RS00300 | 53177..53476 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTY27_RS00305 | 53544..53825 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTY27_RS00310 | 53815..54066 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTY27_RS00315 | 54166..54801 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTY27_RS00320 | 54874..55161 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTY27_RS00325 | 55174..55428 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTY27_RS00330 | 55430..56071 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| HTY27_RS00335 | 56077..57072 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HTY27_RS00340 | 57076..57333 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTY27_RS00345 | 57330..57683 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTY27_RS00350 | 57903..58349 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTY27_RS00355 | 58360..58530 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTY27_RS00360 | 58534..58977 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTY27_RS00370 | 60798..61640 | - | 843 | Protein_77 | SPFH domain-containing protein | - |
| HTY27_RS00375 | 61667..61843 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTY27_RS00380 | 61836..62051 | - | 216 | WP_001127357 | DUF1187 family protein | - |
Host bacterium
| ID | 4718 | GenBank | NZ_MN232214 |
| Plasmid name | pHLJ179-85 | Incompatibility group | IncI2 |
| Plasmid size | 63140 bp | Coordinate of oriT [Strand] | 13631..13683 [-] |
| Host baterium | Escherichia fergusonii |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |