Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104270 |
| Name | oriT_pHLJ179-32  |
| Organism | Escherichia fergusonii |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN232212 (14218..14270 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pHLJ179-32
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3037 | GenBank | WP_015059539 |
| Name | t4cp2_HTX71_RS00255_pHLJ179-32 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34711..60167
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTX71_RS00180 | 29933..31057 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTX71_RS00425 | 31390..31608 | + | 219 | Protein_39 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTX71_RS00190 | 32229..32384 | - | 156 | WP_001358489 | hypothetical protein | - |
| HTX71_RS00195 | 32773..34059 | - | 1287 | WP_104730792 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTX71_RS00200 | 34072..34707 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTX71_RS00205 | 34711..35193 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTX71_RS00210 | 35259..35822 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| HTX71_RS00215 | 35872..36981 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTX71_RS00220 | 36972..38510 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTX71_RS00225 | 38535..39029 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTX71_RS00230 | 39013..40335 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTX71_RS00235 | 40374..40589 | - | 216 | WP_104920580 | hypothetical protein | - |
| HTX71_RS00240 | 40555..41767 | - | 1213 | Protein_50 | IS3 family transposase | - |
| HTX71_RS00245 | 41773..43329 | - | 1557 | WP_104920599 | PilN family type IVB pilus formation outer membrane protein | - |
| HTX71_RS00250 | 43322..43858 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTX71_RS00255 | 43905..45863 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTX71_RS00260 | 45879..46934 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTX71_RS00265 | 46953..48092 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| HTX71_RS00270 | 48082..48633 | - | 552 | WP_104920598 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTX71_RS00275 | 48595..49808 | + | 1214 | WP_162842843 | IS3 family transposase | - |
| HTX71_RS00280 | 49848..50165 | - | 318 | WP_238784485 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTX71_RS00285 | 50162..50896 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTX71_RS00290 | 51062..53419 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| HTX71_RS00295 | 53425..53745 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTX71_RS00420 | 53816..54106 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTX71_RS00305 | 54152..54691 | - | 540 | WP_238784486 | lytic transglycosylase domain-containing protein | virB1 |
| HTX71_RS00310 | 54712..55110 | - | 399 | WP_001153669 | hypothetical protein | - |
| HTX71_RS00315 | 55229..55666 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| HTX71_RS00320 | 55672..56907 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| HTX71_RS00325 | 56910..57209 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTX71_RS00330 | 57257..58066 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| HTX71_RS00335 | 58289..58513 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| HTX71_RS00340 | 58522..59166 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| HTX71_RS00345 | 59172..60167 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| HTX71_RS00350 | 60171..60428 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTX71_RS00355 | 60425..60778 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTX71_RS00360 | 60998..61444 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTX71_RS00365 | 61455..61625 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTX71_RS00370 | 61629..62072 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTX71_RS00375 | 62446..63399 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTX71_RS00380 | 63426..63602 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTX71_RS00385 | 63595..63810 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTX71_RS00390 | 63803..64309 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 4709 | GenBank | NZ_MN232212 |
| Plasmid name | pHLJ179-32 | Incompatibility group | IncI2 |
| Plasmid size | 64899 bp | Coordinate of oriT [Strand] | 14218..14270 [-] |
| Host baterium | Escherichia fergusonii |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |