Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104250 |
| Name | oriT_pMCR-M21015  |
| Organism | Citrobacter amalonaticus strain M21015 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MK041211 (13136..13188 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pMCR-M21015
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 3024 | GenBank | WP_015059539 |
| Name | t4cp2_HTU59_RS00245_pMCR-M21015 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34207..57664
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTU59_RS00175 | 29682..30806 | - | 1125 | WP_000486716 | site-specific integrase | - |
| HTU59_RS00180 | 31129..31284 | - | 156 | WP_001358489 | hypothetical protein | - |
| HTU59_RS00185 | 31642..31902 | - | 261 | WP_029237379 | hypothetical protein | - |
| HTU59_RS00435 | 31961..32182 | + | 222 | Protein_40 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTU59_RS00195 | 32179..33555 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTU59_RS00200 | 33568..34203 | - | 636 | WP_000934977 | A24 family peptidase | - |
| HTU59_RS00205 | 34207..34689 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| HTU59_RS00210 | 34755..35312 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| HTU59_RS00215 | 35357..36466 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| HTU59_RS00220 | 36457..37995 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTU59_RS00225 | 38020..38514 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| HTU59_RS00230 | 38498..39820 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| HTU59_RS00235 | 39859..41502 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| HTU59_RS00240 | 41495..42031 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| HTU59_RS00245 | 42078..44036 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| HTU59_RS00250 | 44052..45107 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTU59_RS00255 | 45126..46265 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| HTU59_RS00260 | 46255..46956 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HTU59_RS00265 | 47022..47756 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HTU59_RS00270 | 47922..50279 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| HTU59_RS00275 | 50285..50605 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| HTU59_RS00420 | 50676..50966 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HTU59_RS00285 | 50966..51550 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| HTU59_RS00290 | 51571..51969 | - | 399 | WP_001153665 | hypothetical protein | - |
| HTU59_RS00295 | 52088..52525 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| HTU59_RS00300 | 52531..53766 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| HTU59_RS00305 | 53769..54068 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTU59_RS00310 | 54136..54417 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTU59_RS00315 | 54407..54658 | - | 252 | WP_000121741 | hypothetical protein | - |
| HTU59_RS00320 | 54758..55393 | - | 636 | WP_015059536 | hypothetical protein | - |
| HTU59_RS00325 | 55466..55753 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| HTU59_RS00330 | 55766..56020 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| HTU59_RS00335 | 56022..56663 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| HTU59_RS00340 | 56669..57664 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| HTU59_RS00345 | 57668..57925 | - | 258 | WP_000739144 | hypothetical protein | - |
| HTU59_RS00350 | 57922..58275 | - | 354 | WP_223286767 | hypothetical protein | - |
| HTU59_RS00355 | 58495..58941 | - | 447 | WP_001243165 | hypothetical protein | - |
| HTU59_RS00360 | 58952..59122 | - | 171 | WP_000550720 | hypothetical protein | - |
| HTU59_RS00365 | 59126..59569 | - | 444 | WP_000964330 | NfeD family protein | - |
| HTU59_RS00370 | 59943..60896 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| HTU59_RS00375 | 60923..61099 | - | 177 | WP_000753050 | hypothetical protein | - |
| HTU59_RS00380 | 61092..61307 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| HTU59_RS00385 | 61300..61806 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 4689 | GenBank | NZ_MK041211 |
| Plasmid name | pMCR-M21015 | Incompatibility group | IncI2 |
| Plasmid size | 62891 bp | Coordinate of oriT [Strand] | 13136..13188 [-] |
| Host baterium | Citrobacter amalonaticus strain M21015 |
Cargo genes
| Drug resistance gene | mcr-1.5 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |