Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104249
Name   oriT_pLd10 in_silico
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03P
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MG813924 (19154..19290 [-], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pLd10
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3121 GenBank   WP_023349257
Name   Relaxase_HTQ93_RS00105_pLd10 insolico UniProt ID   A0A2R4AKS0
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47692.33 Da        Isoelectric Point: 8.1119

>WP_023349257.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWLILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB A0A2R4AKS0


Host bacterium

ID   4688 GenBank   NZ_MG813924
Plasmid name   pLd10 Incompatibility group   -
Plasmid size   39563 bp Coordinate of oriT [Strand]   19154..19290 [-]
Host baterium   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03P

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -