Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104246
Name   oriT_pMTY10660_IncW in_silico
Organism   Enterobacter hormaechei strain TUM10660
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP018350 (12218..12318 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      39..44, 49..54  (CGCACC..GGTGCG)
 13..21, 27..35  (AAGTCATTG..CAATGACTT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  TGTCTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pMTY10660_IncW
TCCTTGGCGTGGAAGTCATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTAAGGATACCAACCCGGCTTTTAAGGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3119 GenBank   WP_065164176
Name   mobF_CPI47_RS00105_pMTY10660_IncW insolico UniProt ID   _
Length   962 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 962 a.a.        Molecular weight: 106978.20 Da        Isoelectric Point: 9.8804

>WP_065164176.1 MULTISPECIES: MobF family relaxase [Pseudomonadota]
MVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLAGNIGEGHRIMRSAT
RQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQAEARAQARQKIQGKTRIETTGNLV
IGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRALKNDEIVKATRYLGAVYNAELAHELQKLGYQLRYG
KDGNFDLAHIDRQQIEGFSKRTEQIAEWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQAT
AKELGIDFSRREWSGREKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAA
RLEDIQKELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWVAELAAKGMKQGAARERVDNAIKTGG
LVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREAAELIVSAANRVVGV
QGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEANTLASFLRAKDKNIDSRTVLVIDE
AGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIEAGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVE
LAAAGKASSSLERIKDVTEIKNHHERRAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAG
KGIEFDTLVRVDTTQAERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQ
FSPMTHTKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTDKPLH
VDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGKLPAAIARENIKSAA
HDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAERGMEAGR

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(9-283)

AAA domain (AAA_30)

(469-653)


  Protein structure



No available structure.




Auxiliary protein

ID   1362 GenBank   WP_012196434
Name   WP_012196434_pMTY10660_IncW insolico UniProt ID   A8R762
Length   121 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 121 a.a.        Molecular weight: 13400.32 Da        Isoelectric Point: 4.8464

>WP_012196434.1 MULTISPECIES: hypothetical protein [Pseudomonadota]
MALGDPIQVRLSPEKQALLEDEAARKGKRLATYLRELLESENDLQGELAALRREVVSLHHVIEDLADTGL
RSDQSGPGQNAVQIETLLLLRAIAGPERMKPVKGELKRLGIEVWTPEGKED

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A8R762


T4CP

ID   3022 GenBank   WP_012196433
Name   t4cp2_CPI47_RS00100_pMTY10660_IncW insolico UniProt ID   _
Length   507 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 507 a.a.        Molecular weight: 56272.95 Da        Isoelectric Point: 9.8647

>WP_012196433.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Pseudomonadota]
MHPDDQRKVSAGIVIVLPLIFWITAVQKTEVLGSPKLLALWELMKLTPQKPILLLSALGGLAVGVLFVWL
LNSVGQGEFGGAPFKRFLRGTRIVSGGKLKRMTREKAKQVTVAGVPMPRDAEPRHLLVNGATGTGKSVLL
RELAYTGLLRGDRMVIVDPNGDMLSKFGRDKDIILNPYDQRTKGWSFFNEIRNDYDWQRYALSVVPRGKT
DEAEEWASYGRLLLRETAKKLALIGTPSMRELFHWTTIATFDDLRGFLEGTLAESLFAGSNEASKALTSA
RFVLSDKLPEHVTMPDGDFSIRSWLEDPNGGNLFITWREDMGPALRPLISAWVDVVCTSILSLPEEPKRR
LWLFIDELASLEKLASLADALTKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASFRSLVVLGGSRTDP
KTNEDMSLSLGEHEVERDRYSKNTGKHHSTGRALERVRERVVMPAEIANLPDLTAYVGFAGNRPIAKVPL
EIKQFANRQPAFVEGTI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(110-490)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12780..27417

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPI47_RS00060 (TUM10660_00011) 7860..8183 + 324 WP_012196442 TrfB-related DNA-binding protein -
CPI47_RS00065 (TUM10660_00012) 8876..9220 + 345 WP_012196440 hypothetical protein -
CPI47_RS00070 (TUM10660_00013) 9236..9889 + 654 WP_012196439 hypothetical protein -
CPI47_RS00075 (TUM10660_00014) 10037..10288 + 252 WP_012196438 hypothetical protein -
CPI47_RS00080 (TUM10660_00015) 10442..10828 - 387 WP_012196437 hypothetical protein -
CPI47_RS00085 (TUM10660_00016) 10842..11537 - 696 WP_012196436 StbB family protein -
CPI47_RS00090 (TUM10660_00017) 11534..11959 - 426 WP_012196435 hypothetical protein -
CPI47_RS00095 (TUM10660_00018) 12413..12778 + 366 WP_012196434 hypothetical protein -
CPI47_RS00100 (TUM10660_00019) 12780..14303 + 1524 WP_012196433 type IV secretion system DNA-binding domain-containing protein virb4
CPI47_RS00105 (TUM10660_00020) 14315..17203 + 2889 WP_065164176 MobF family relaxase -
CPI47_RS00110 (TUM10660_00021) 17286..18362 - 1077 WP_012196431 P-type DNA transfer ATPase VirB11 virB11
CPI47_RS00115 (TUM10660_00022) 18328..19515 - 1188 WP_012196430 type IV secretion system protein VirB10 virB10
CPI47_RS00120 (TUM10660_00023) 19515..20315 - 801 WP_012196429 P-type conjugative transfer protein VirB9 virB9
CPI47_RS00125 (TUM10660_00024) 20326..21021 - 696 WP_012196428 VirB8/TrbF family protein virB8
CPI47_RS00135 (TUM10660_00026) 21274..22302 - 1029 WP_012196426 type IV secretion system protein virB6
CPI47_RS00140 (TUM10660_00027) 22316..22546 - 231 WP_012196425 EexN family lipoprotein -
CPI47_RS00145 (TUM10660_00028) 22543..23232 - 690 WP_012196424 P-type DNA transfer protein VirB5 virB5
CPI47_RS00150 (TUM10660_00029) 23229..25700 - 2472 WP_012196423 VirB4 family type IV secretion/conjugal transfer ATPase virb4
CPI47_RS00155 (TUM10660_00030) 25703..26017 - 315 WP_012196422 VirB3 family type IV secretion system protein virB3
CPI47_RS00160 (TUM10660_00031) 26030..26335 - 306 WP_031942907 VirB2 family type IV secretion system major pilin TrwL virB2
CPI47_RS00165 (TUM10660_00032) 26301..26597 - 297 WP_012196420 KorA family transcriptional regulator -
CPI47_RS00170 (TUM10660_00033) 26719..27417 - 699 WP_012443549 lytic transglycosylase domain-containing protein virB1
CPI47_RS00175 (TUM10660_00034) 27419..28000 - 582 WP_012196419 hypothetical protein -
CPI47_RS00180 (TUM10660_00035) 28149..28436 + 288 WP_012414174 H-NS histone family protein -
CPI47_RS00185 (TUM10660_00036) 28733..29233 - 501 WP_000376623 GNAT family N-acetyltransferase -
CPI47_RS00190 (TUM10660_00037) 29361..29864 - 504 Protein_36 dihydropteroate synthase -
CPI47_RS00195 (TUM10660_00038) 29865..30419 - 555 WP_012695458 AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase -
CPI47_RS00200 (TUM10660_00039) 30605..31345 - 741 WP_003159548 subclass B1 metallo-beta-lactamase IMP-1 -

Host bacterium

ID   4685 GenBank   NZ_AP018350
Plasmid name   pMTY10660_IncW Incompatibility group   IncW
Plasmid size   33748 bp Coordinate of oriT [Strand]   12218..12318 [-]
Host baterium   Enterobacter hormaechei strain TUM10660

Cargo genes

Drug resistance gene   blaIMP-1
Virulence gene   trwD, trwF, trwG, trwH1, trwK, trwM
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -