Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104228 |
| Name | oriT_p2327  |
| Organism | Burkholderia pyrrocinia strain DSM 10685 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011506 (72097..72797 [-], 701 nt) |
| oriT length | 701 nt |
| IRs (inverted repeats) | 597..602, 607..612 (GCGGGG..CCCCGC) 535..540, 545..550 (CGCGCC..GGCGCG) 234..239, 241..246 (CGACAA..TTGTCG) 175..183, 197..205 (TGTCAGACA..TGTCTGACA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 701 nt
>oriT_p2327
GAAAAGCGCCTGGAATGAAAAGAGCGGGCGCTCGTGGAGTCGAGGAATAACCTCGGCTCGCGCACGAAACCGCGCTCGATTACGGCCCTCACGACTGCATTATTTTGATCGACAGTAGCACGACGAAGAGCGTTTCGCAATAACTCATTTATTTTCATAGTGATACTTATCGAATGTCAGACACAGCCGTTCTAGCTGTCTGACAACGTGCCCCAATCGTGACGGGGTGGGCGCGACAAATTGTCGATGTTCGCGCTTTCCGGCCATTCGGGTGTCTGACAGCGTGAACCTTAACGCAAGCGGGCTTTCGCCATACGTTGCGGCGTTTTCGCGACAGGTCGACTGTAGCTGTCTGACGCTGCACACCGCCACCGTCAGTGGCCGCGCGGCGATTCTCTTTTCATTTGGATTCCGAATTATAATTTCCAATGCCATATTTGTCTGACACCGGAGGATATGACGTACAAGAGGTTGCGCTTGATCCGCACCTCGCTTTTTTCGCTTATTTTCGTAGGTGTCTGACGCCGAAGGCACCGCGCCATGCGGCGCGACACACCGCAACAACGTTGCATGGTGTGTGACGATCGTCTTAGGGGGCGGGGATTCCCCCGCTTCCTAAGACGATCGTAGCCAACTTCACTTCAGGTTTGGGCCGGTCGCGGAGCCTTCACCCGGTAAGGTACGGCGGTTCCCGCTCAAGG
GAAAAGCGCCTGGAATGAAAAGAGCGGGCGCTCGTGGAGTCGAGGAATAACCTCGGCTCGCGCACGAAACCGCGCTCGATTACGGCCCTCACGACTGCATTATTTTGATCGACAGTAGCACGACGAAGAGCGTTTCGCAATAACTCATTTATTTTCATAGTGATACTTATCGAATGTCAGACACAGCCGTTCTAGCTGTCTGACAACGTGCCCCAATCGTGACGGGGTGGGCGCGACAAATTGTCGATGTTCGCGCTTTCCGGCCATTCGGGTGTCTGACAGCGTGAACCTTAACGCAAGCGGGCTTTCGCCATACGTTGCGGCGTTTTCGCGACAGGTCGACTGTAGCTGTCTGACGCTGCACACCGCCACCGTCAGTGGCCGCGCGGCGATTCTCTTTTCATTTGGATTCCGAATTATAATTTCCAATGCCATATTTGTCTGACACCGGAGGATATGACGTACAAGAGGTTGCGCTTGATCCGCACCTCGCTTTTTTCGCTTATTTTCGTAGGTGTCTGACGCCGAAGGCACCGCGCCATGCGGCGCGACACACCGCAACAACGTTGCATGGTGTGTGACGATCGTCTTAGGGGGCGGGGATTCCCCCGCTTCCTAAGACGATCGTAGCCAACTTCACTTCAGGTTTGGGCCGGTCGCGGAGCCTTCACCCGGTAAGGTACGGCGGTTCCCGCTCAAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3102 | GenBank | WP_047904789 |
| Name | mobF_ABD05_RS35055_p2327 |
UniProt ID | A0A0G3X3H4 |
| Length | 939 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 939 a.a. Molecular weight: 104758.57 Da Isoelectric Point: 6.5502
>WP_047904789.1 MobF family relaxase [Burkholderia pyrrocinia]
MLSLHNIASAAQTRNYFSKDNYYTQDENTEHSAWFGAGAARLGLAGQVQPAAFLNLLEGRVEDQQLGKWV
TDEETGERRQDHQPGIDITFSAPKSVSLMAEVAGDTAVRDAHEAAVRSALAYLEENVIRTRSTQNGVTSI
EHTGNMVAALFKHNTSRDLDPQTHTHAVVMNVTLRPDGKWRSIDNREIYQTQKLAGAIYNAELASGLQQL
GYRLTNPDRHGNFEIQGISREQIEHFSQRRTAMKEKLGKRQLELDTASAADKERAALATRERKVDVDHEQ
LLADWQARALEQGIDLGVLRAAAGERKAQGIDAPTKLTGRQALAFAAAHLIEREAVVDKSNLVETAVVHG
TGRVSPQDVMKAFRQLEKNGDLVPLPDEKYTTRKMLESERWALDFVKQEKGNAPVVLEAPKVQERIAEAE
RLQQFSYTFGQRDAIVLALTSKDRFVAIQGLAGTGKTTMLRTLNDIAAAEGYVVRGMAPTGAAAKVMVKE
TGIAAETVAMFQIKERQLQKDIEFAQQYAPDFKRKPEVWVVDESSFLAQRQMAQLHRLAEHAGAKVVYLG
DTLQLQGVEAGKPFELAQKDGIATAYMTDINRQKTDVMKEAVDIITGRNQLDAGARLTDIELKRNAQSFQ
YLSQQGRVHQTDEVVTSTVDKILAMSPEERARNITITPLNRDRVAINEGVRVGLQARGELSKNESTHEIL
VSKGFTRAQLKEAQYYERGDVVRFNRTYQQINADKGDYTRVIDVDHESGIVTLRKRDGSTLAWEPAKHNK
VEVYDPDLRQLAVGDVIRLTRNDETFKNGETARVVQLTGEHAVLQVDDRGVISHHSVNLETSQHWDHAYA
STVHAAQGASRNTTIFAINMPDNDDKKAQEREFQALAKVFGDRSFYVGVTRASHDISIVTNDIDLAQRAI
TGKLDKTTVIEAMNQEVREQTIQREQHEI
MLSLHNIASAAQTRNYFSKDNYYTQDENTEHSAWFGAGAARLGLAGQVQPAAFLNLLEGRVEDQQLGKWV
TDEETGERRQDHQPGIDITFSAPKSVSLMAEVAGDTAVRDAHEAAVRSALAYLEENVIRTRSTQNGVTSI
EHTGNMVAALFKHNTSRDLDPQTHTHAVVMNVTLRPDGKWRSIDNREIYQTQKLAGAIYNAELASGLQQL
GYRLTNPDRHGNFEIQGISREQIEHFSQRRTAMKEKLGKRQLELDTASAADKERAALATRERKVDVDHEQ
LLADWQARALEQGIDLGVLRAAAGERKAQGIDAPTKLTGRQALAFAAAHLIEREAVVDKSNLVETAVVHG
TGRVSPQDVMKAFRQLEKNGDLVPLPDEKYTTRKMLESERWALDFVKQEKGNAPVVLEAPKVQERIAEAE
RLQQFSYTFGQRDAIVLALTSKDRFVAIQGLAGTGKTTMLRTLNDIAAAEGYVVRGMAPTGAAAKVMVKE
TGIAAETVAMFQIKERQLQKDIEFAQQYAPDFKRKPEVWVVDESSFLAQRQMAQLHRLAEHAGAKVVYLG
DTLQLQGVEAGKPFELAQKDGIATAYMTDINRQKTDVMKEAVDIITGRNQLDAGARLTDIELKRNAQSFQ
YLSQQGRVHQTDEVVTSTVDKILAMSPEERARNITITPLNRDRVAINEGVRVGLQARGELSKNESTHEIL
VSKGFTRAQLKEAQYYERGDVVRFNRTYQQINADKGDYTRVIDVDHESGIVTLRKRDGSTLAWEPAKHNK
VEVYDPDLRQLAVGDVIRLTRNDETFKNGETARVVQLTGEHAVLQVDDRGVISHHSVNLETSQHWDHAYA
STVHAAQGASRNTTIFAINMPDNDDKKAQEREFQALAKVFGDRSFYVGVTRASHDISIVTNDIDLAQRAI
TGKLDKTTVIEAMNQEVREQTIQREQHEI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0G3X3H4 |
T4CP
| ID | 3006 | GenBank | WP_047904788 |
| Name | t4cp2_ABD05_RS35045_p2327 |
UniProt ID | _ |
| Length | 639 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 639 a.a. Molecular weight: 71835.62 Da Isoelectric Point: 6.9126
>WP_047904788.1 type IV secretion system DNA-binding domain-containing protein [Burkholderia pyrrocinia]
MTGKKPNGLFQDFSTGSEIWTHRVALVLVAVRNILLLSLFLGLAAGFIYAAFFVDRDIWRPAVANVIAHL
RSVVMMTAVPMEYEVNGQTVRLPVDQVAALTDPVWDVAYFFLRRFLIIVGTVTACTCVSIAAYWYEFGRS
TMADKDLRGATLVNGKQLAKMIRQDDMESPYSIAGVPMLRNAETLHTLVVGAQGTGKSQQFFDRLRQVRA
RGKRAIVYDPSGEFAEEFFREGKDVMMNPFDDRSPNWNIKYEIEEEYHYDSMATGLIPDPREADPFWSLA
GREVFRDVARTLNKEGNLTNRNLYNSIAMSNLDAIYQLLRNTAGASYVDPKTERTGMSLKMTVQNQLSSF
RFLRDDGKPFSIRQWIYNESDSWMFISTREEMLEAIKPIMSLWIDIAIKAVLNLTPIHRERLHFSIDEVP
TLQKLDIMKLAVSNTRKYGLCMMLGAQDMPQFLDIYGEYLARTIINGCQTKLLLRVTDAEAAEMFSKIIG
QTEVEEKDESLSFGVNSQRDGFSIAKRRLLRDLALPSQILRLPDMTGYLVYPGEYPIARVKYGFVKPRKN
APAFIKRQAFSELFAPAAGLPATAAASASVVVGPSPDWIAHPEILVAASGGVDPETGEILPPRPGAWRWG
DGNQRGDHV
MTGKKPNGLFQDFSTGSEIWTHRVALVLVAVRNILLLSLFLGLAAGFIYAAFFVDRDIWRPAVANVIAHL
RSVVMMTAVPMEYEVNGQTVRLPVDQVAALTDPVWDVAYFFLRRFLIIVGTVTACTCVSIAAYWYEFGRS
TMADKDLRGATLVNGKQLAKMIRQDDMESPYSIAGVPMLRNAETLHTLVVGAQGTGKSQQFFDRLRQVRA
RGKRAIVYDPSGEFAEEFFREGKDVMMNPFDDRSPNWNIKYEIEEEYHYDSMATGLIPDPREADPFWSLA
GREVFRDVARTLNKEGNLTNRNLYNSIAMSNLDAIYQLLRNTAGASYVDPKTERTGMSLKMTVQNQLSSF
RFLRDDGKPFSIRQWIYNESDSWMFISTREEMLEAIKPIMSLWIDIAIKAVLNLTPIHRERLHFSIDEVP
TLQKLDIMKLAVSNTRKYGLCMMLGAQDMPQFLDIYGEYLARTIINGCQTKLLLRVTDAEAAEMFSKIIG
QTEVEEKDESLSFGVNSQRDGFSIAKRRLLRDLALPSQILRLPDMTGYLVYPGEYPIARVKYGFVKPRKN
APAFIKRQAFSELFAPAAGLPATAAASASVVVGPSPDWIAHPEILVAASGGVDPETGEILPPRPGAWRWG
DGNQRGDHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 85654..112534
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ABD05_RS35070 (ABD05_35060) | 81645..82673 | + | 1029 | WP_047904791 | LuxR C-terminal-related transcriptional regulator | - |
| ABD05_RS35075 (ABD05_35065) | 82795..83835 | + | 1041 | WP_047904792 | TRAP transporter substrate-binding protein DctP | - |
| ABD05_RS35080 (ABD05_35070) | 83832..84068 | + | 237 | WP_047904793 | hypothetical protein | - |
| ABD05_RS35085 (ABD05_35075) | 84055..84825 | + | 771 | WP_047904794 | 5-oxoprolinase subunit PxpA | - |
| ABD05_RS35090 (ABD05_35080) | 84947..85219 | + | 273 | WP_047904795 | hypothetical protein | - |
| ABD05_RS35095 (ABD05_35085) | 85654..86130 | + | 477 | WP_047904796 | lytic transglycosylase domain-containing protein | virB1 |
| ABD05_RS37220 | 86127..86471 | + | 345 | WP_082146353 | hypothetical protein | - |
| ABD05_RS35100 (ABD05_35090) | 86500..86892 | + | 393 | WP_006487426 | hypothetical protein | - |
| ABD05_RS35105 (ABD05_35095) | 86906..87199 | + | 294 | WP_006487365 | type IV conjugative transfer system protein TraL | traL |
| ABD05_RS35110 (ABD05_35100) | 87206..87796 | + | 591 | WP_047904797 | TraE/TraK family type IV conjugative transfer system protein | traE |
| ABD05_RS35115 (ABD05_35105) | 87735..88481 | + | 747 | WP_047904798 | type-F conjugative transfer system secretin TraK | traK |
| ABD05_RS35120 (ABD05_35110) | 88474..89907 | + | 1434 | WP_047904799 | TraB/VirB10 family protein | traB |
| ABD05_RS35125 (ABD05_35115) | 89900..90694 | + | 795 | WP_053060048 | DsbC family protein | trbB |
| ABD05_RS35130 (ABD05_35120) | 90691..91356 | + | 666 | WP_047904800 | type IV conjugative transfer system lipoprotein TraV | traV |
| ABD05_RS35135 (ABD05_35125) | 91359..93965 | + | 2607 | WP_047904801 | type IV secretion system protein TraC | virb4 |
| ABD05_RS35140 (ABD05_35130) | 93962..94477 | + | 516 | WP_082146354 | hypothetical protein | - |
| ABD05_RS35145 (ABD05_35135) | 94474..95214 | + | 741 | WP_047904803 | hypothetical protein | - |
| ABD05_RS39255 (ABD05_35140) | 95217..95825 | + | 609 | WP_047904804 | hypothetical protein | - |
| ABD05_RS39260 | 95822..96595 | + | 774 | WP_238594246 | hypothetical protein | - |
| ABD05_RS35160 (ABD05_35150) | 96592..96957 | + | 366 | WP_047904805 | hypothetical protein | - |
| ABD05_RS35165 (ABD05_35155) | 96988..97566 | + | 579 | WP_053060050 | phospholipase D family protein | - |
| ABD05_RS35170 (ABD05_35160) | 97580..98392 | + | 813 | WP_082146355 | GntR family transcriptional regulator | - |
| ABD05_RS37230 | 98370..98588 | + | 219 | WP_082146356 | hypothetical protein | - |
| ABD05_RS35175 (ABD05_35165) | 98606..99136 | + | 531 | WP_053060051 | signal peptidase I | - |
| ABD05_RS35180 (ABD05_35170) | 99123..99761 | + | 639 | WP_047904807 | type-F conjugative transfer system protein TraW | traW |
| ABD05_RS35185 (ABD05_35175) | 99779..100768 | + | 990 | WP_148669221 | conjugal transfer pilus assembly protein TraU | traU |
| ABD05_RS35190 (ABD05_35180) | 100765..101466 | + | 702 | WP_047904809 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| ABD05_RS35195 (ABD05_35185) | 101468..101845 | + | 378 | WP_082146357 | hypothetical protein | - |
| ABD05_RS35200 (ABD05_35190) | 101847..102968 | + | 1122 | WP_053060052 | hypothetical protein | - |
| ABD05_RS35205 (ABD05_35195) | 102976..104220 | + | 1245 | WP_047904811 | conjugal transfer protein TraN | traN |
| ABD05_RS38485 | 104217..104483 | + | 267 | WP_158361742 | hypothetical protein | - |
| ABD05_RS35210 (ABD05_35200) | 104480..104788 | + | 309 | WP_047904812 | hypothetical protein | - |
| ABD05_RS35215 (ABD05_35205) | 104785..105174 | + | 390 | WP_006493947 | hypothetical protein | - |
| ABD05_RS35220 (ABD05_35210) | 105179..106012 | + | 834 | WP_047904813 | conjugal transfer protein TraF | traF |
| ABD05_RS35225 (ABD05_35215) | 106023..106553 | + | 531 | WP_047904814 | hypothetical protein | - |
| ABD05_RS35230 (ABD05_35220) | 106550..106993 | + | 444 | WP_006487377 | HNH endonuclease | - |
| ABD05_RS35235 (ABD05_35225) | 106990..108024 | + | 1035 | WP_047904815 | phage Gp37/Gp68 family protein | - |
| ABD05_RS35240 (ABD05_35230) | 108017..109471 | + | 1455 | WP_047904832 | conjugal transfer protein TraH | traH |
| ABD05_RS35245 (ABD05_35235) | 109481..112534 | + | 3054 | WP_047904745 | conjugal transfer protein TraG N-terminal domain-containing protein | traG |
| ABD05_RS35250 (ABD05_35240) | 112524..112904 | - | 381 | WP_020980049 | hypothetical protein | - |
Host bacterium
| ID | 4667 | GenBank | NZ_CP011506 |
| Plasmid name | p2327 | Incompatibility group | - |
| Plasmid size | 113484 bp | Coordinate of oriT [Strand] | 72097..72797 [-] |
| Host baterium | Burkholderia pyrrocinia strain DSM 10685 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |