Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104187 |
| Name | oriT_pKLP00187_2  |
| Organism | Klebsiella pneumoniae strain KLP00187 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OP378659 (157073..157122 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKLP00187_2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3077 | GenBank | WP_015065542 |
| Name | traI_PWC64_RS00690_pKLP00187_2 |
UniProt ID | K7R3U7 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190318.03 Da Isoelectric Point: 6.2890
>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2975 | GenBank | WP_015065541 |
| Name | traD_PWC64_RS00695_pKLP00187_2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86025.00 Da Isoelectric Point: 5.0577
>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 127653..157680
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWC64_RS00695 | 127653..129965 | - | 2313 | WP_015065541 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PWC64_RS00700 | 130094..130783 | - | 690 | WP_072198073 | hypothetical protein | - |
| PWC64_RS00705 | 130976..131707 | - | 732 | WP_016831056 | conjugal transfer complement resistance protein TraT | - |
| PWC64_RS00710 | 131958..132560 | - | 603 | WP_071604857 | hypothetical protein | - |
| PWC64_RS00715 | 132583..135405 | - | 2823 | WP_016831055 | conjugal transfer mating-pair stabilization protein TraG | traG |
| PWC64_RS00720 | 135405..136784 | - | 1380 | WP_072159542 | conjugal transfer pilus assembly protein TraH | traH |
| PWC64_RS00725 | 136762..137205 | - | 444 | WP_016831053 | F-type conjugal transfer protein TrbF | - |
| PWC64_RS00730 | 137251..137808 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PWC64_RS00735 | 137780..138019 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PWC64_RS00740 | 138030..138782 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PWC64_RS00745 | 138803..139129 | - | 327 | WP_016831051 | hypothetical protein | - |
| PWC64_RS00750 | 139142..139390 | - | 249 | WP_004152675 | hypothetical protein | - |
| PWC64_RS00755 | 139368..139622 | - | 255 | WP_015065558 | conjugal transfer protein TrbE | - |
| PWC64_RS00760 | 139654..141609 | - | 1956 | WP_023313940 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PWC64_RS00765 | 141668..142315 | - | 648 | WP_039109927 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| PWC64_RS00770 | 142591..143580 | - | 990 | WP_015632509 | conjugal transfer pilus assembly protein TraU | traU |
| PWC64_RS00775 | 143577..143978 | - | 402 | WP_016831047 | hypothetical protein | - |
| PWC64_RS00780 | 144013..144648 | - | 636 | WP_016831046 | type-F conjugative transfer system protein TraW | traW |
| PWC64_RS00785 | 144648..145037 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| PWC64_RS00790 | 145037..147676 | - | 2640 | WP_016831045 | type IV secretion system protein TraC | virb4 |
| PWC64_RS00795 | 147748..148146 | - | 399 | WP_023158008 | hypothetical protein | - |
| PWC64_RS00800 | 148154..148447 | - | 294 | WP_020325117 | hypothetical protein | - |
| PWC64_RS00805 | 148587..148991 | - | 405 | WP_016831042 | hypothetical protein | - |
| PWC64_RS00810 | 149058..149369 | - | 312 | WP_016831041 | hypothetical protein | - |
| PWC64_RS00815 | 149370..149588 | - | 219 | WP_014386199 | hypothetical protein | - |
| PWC64_RS00820 | 149612..149901 | - | 290 | Protein_163 | hypothetical protein | - |
| PWC64_RS00825 | 149906..150316 | - | 411 | WP_032409654 | hypothetical protein | - |
| PWC64_RS00830 | 150448..151032 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| PWC64_RS00835 | 151055..151651 | - | 597 | Protein_166 | conjugal transfer pilus-stabilizing protein TraP | - |
| PWC64_RS00840 | 151644..153068 | - | 1425 | WP_016831036 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PWC64_RS00845 | 153068..153808 | - | 741 | WP_032408905 | type-F conjugative transfer system secretin TraK | traK |
| PWC64_RS00850 | 153795..154361 | - | 567 | WP_016831035 | type IV conjugative transfer system protein TraE | traE |
| PWC64_RS00855 | 154381..154686 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| PWC64_RS00860 | 154700..155068 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| PWC64_RS00865 | 155130..155336 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| PWC64_RS00870 | 155469..156188 | - | 720 | WP_016831034 | conjugal transfer protein TrbJ | - |
| PWC64_RS00875 | 156388..156804 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PWC64_RS00880 | 157195..157680 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| PWC64_RS00885 | 157713..158042 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| PWC64_RS00890 | 158075..158896 | - | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| PWC64_RS00895 | 159729..160142 | - | 414 | WP_004182074 | helix-turn-helix domain-containing protein | - |
| PWC64_RS00900 | 160143..160421 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| PWC64_RS00905 | 160411..160731 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| PWC64_RS00910 | 160812..161036 | - | 225 | WP_004152719 | hypothetical protein | - |
| PWC64_RS00915 | 161047..161259 | - | 213 | WP_004182070 | hypothetical protein | - |
| PWC64_RS00920 | 161320..161676 | - | 357 | WP_004152717 | hypothetical protein | - |
| PWC64_RS00925 | 162312..162662 | - | 351 | WP_004182068 | hypothetical protein | - |
Host bacterium
| ID | 4626 | GenBank | NZ_OP378659 |
| Plasmid name | pKLP00187_2 | Incompatibility group | IncFIB |
| Plasmid size | 196742 bp | Coordinate of oriT [Strand] | 157073..157122 [+] |
| Host baterium | Klebsiella pneumoniae strain KLP00187 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15, dfrA14, qnrB1, tet(A), aac(6')-Ib-cr, blaOXA-1, aac(3)-IIa |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |