Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104182 |
| Name | oriT_pKLP00218_2  |
| Organism | Klebsiella pneumoniae strain KLP00218 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OP378663 (141486..141535 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKLP00218_2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3074 | GenBank | WP_015065542 |
| Name | traI_PWC58_RS00600_pKLP00218_2 |
UniProt ID | K7R3U7 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190318.03 Da Isoelectric Point: 6.2890
>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2969 | GenBank | WP_015065541 |
| Name | traD_PWC58_RS00605_pKLP00218_2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86025.00 Da Isoelectric Point: 5.0577
>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 112079..142093
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWC58_RS00605 | 112079..114391 | - | 2313 | WP_015065541 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PWC58_RS00610 | 114520..115209 | - | 690 | WP_015065540 | hypothetical protein | - |
| PWC58_RS00615 | 115401..116132 | - | 732 | WP_032329926 | conjugal transfer complement resistance protein TraT | - |
| PWC58_RS00620 | 116441..116965 | - | 525 | WP_015065538 | hypothetical protein | - |
| PWC58_RS00625 | 116976..119819 | - | 2844 | WP_023157999 | conjugal transfer mating-pair stabilization protein TraG | traG |
| PWC58_RS00630 | 119819..121198 | - | 1380 | WP_023158000 | conjugal transfer pilus assembly protein TraH | traH |
| PWC58_RS00635 | 121176..121619 | - | 444 | WP_023158001 | F-type conjugal transfer protein TrbF | - |
| PWC58_RS00640 | 121665..122222 | - | 558 | WP_023158002 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PWC58_RS00645 | 122194..122433 | - | 240 | WP_023158003 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PWC58_RS00650 | 122444..123196 | - | 753 | WP_015065535 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PWC58_RS00655 | 123217..123543 | - | 327 | WP_015065534 | hypothetical protein | - |
| PWC58_RS00660 | 123556..123804 | - | 249 | WP_004152675 | hypothetical protein | - |
| PWC58_RS00665 | 123782..124036 | - | 255 | WP_015065558 | conjugal transfer protein TrbE | - |
| PWC58_RS00670 | 124068..126023 | - | 1956 | WP_015065533 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PWC58_RS00675 | 126082..126729 | - | 648 | WP_015632510 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| PWC58_RS00680 | 127005..127994 | - | 990 | WP_015632509 | conjugal transfer pilus assembly protein TraU | traU |
| PWC58_RS00685 | 127991..128392 | - | 402 | WP_023158005 | hypothetical protein | - |
| PWC58_RS00690 | 128427..129062 | - | 636 | WP_023158006 | type-F conjugative transfer system protein TraW | traW |
| PWC58_RS00695 | 129062..129451 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| PWC58_RS00700 | 129451..132090 | - | 2640 | WP_023158007 | type IV secretion system protein TraC | virb4 |
| PWC58_RS00705 | 132162..132560 | - | 399 | WP_023158008 | hypothetical protein | - |
| PWC58_RS00710 | 132568..132957 | - | 390 | WP_023158009 | hypothetical protein | - |
| PWC58_RS00715 | 133000..133404 | - | 405 | WP_016831042 | hypothetical protein | - |
| PWC58_RS00720 | 133471..133782 | - | 312 | WP_014386200 | hypothetical protein | - |
| PWC58_RS00725 | 133783..134001 | - | 219 | WP_014386199 | hypothetical protein | - |
| PWC58_RS00730 | 134025..134314 | - | 290 | Protein_145 | hypothetical protein | - |
| PWC58_RS00735 | 134319..134729 | - | 411 | WP_016831039 | hypothetical protein | - |
| PWC58_RS00740 | 134861..135445 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| PWC58_RS00745 | 135468..136064 | - | 597 | Protein_148 | conjugal transfer pilus-stabilizing protein TraP | - |
| PWC58_RS00750 | 136057..137481 | - | 1425 | WP_023158013 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PWC58_RS00755 | 137481..138221 | - | 741 | WP_014386193 | type-F conjugative transfer system secretin TraK | traK |
| PWC58_RS00760 | 138208..138774 | - | 567 | WP_016831035 | type IV conjugative transfer system protein TraE | traE |
| PWC58_RS00765 | 138794..139099 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| PWC58_RS00770 | 139113..139481 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| PWC58_RS00775 | 139543..139749 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| PWC58_RS00780 | 139882..140601 | - | 720 | WP_016831034 | conjugal transfer protein TrbJ | - |
| PWC58_RS00785 | 140801..141217 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PWC58_RS00790 | 141608..142093 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| PWC58_RS00795 | 142126..142455 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| PWC58_RS00800 | 142488..143309 | - | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| PWC58_RS00805 | 144137..144700 | - | 564 | WP_004182054 | methyltransferase | - |
| PWC58_RS00810 | 144748..146103 | - | 1356 | WP_004182052 | DUF3560 domain-containing protein | - |
| PWC58_RS00815 | 146155..146385 | - | 231 | WP_001568051 | hypothetical protein | - |
| PWC58_RS00820 | 146477..146704 | - | 228 | WP_004182050 | hypothetical protein | - |
Host bacterium
| ID | 4621 | GenBank | NZ_OP378663 |
| Plasmid name | pKLP00218_2 | Incompatibility group | IncFIB |
| Plasmid size | 164670 bp | Coordinate of oriT [Strand] | 141486..141535 [+] |
| Host baterium | Klebsiella pneumoniae strain KLP00218 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15, dfrA14 |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |