Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104181 |
| Name | oriT_pKLP00172_2  |
| Organism | Klebsiella pneumoniae strain KLP00172 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OP378654 (136526..136575 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKLP00172_2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3073 | GenBank | WP_004153029 |
| Name | traI_PWC47_RS00545_pKLP00172_2 |
UniProt ID | A0A422WEA5 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190808.90 Da Isoelectric Point: 6.2081
>WP_004153029.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2968 | GenBank | WP_004152630 |
| Name | traD_PWC47_RS00550_pKLP00172_2 |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85951.02 Da Isoelectric Point: 5.0632
>WP_004152630.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 108715..137133
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWC47_RS00550 | 108715..111024 | - | 2310 | WP_004152630 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PWC47_RS00555 | 111384..112115 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| PWC47_RS00560 | 112305..112832 | - | 528 | WP_004153030 | hypothetical protein | - |
| PWC47_RS00565 | 112838..115687 | - | 2850 | WP_004178054 | conjugal transfer mating-pair stabilization protein TraG | traG |
| PWC47_RS00570 | 115687..117057 | - | 1371 | WP_004178055 | conjugal transfer pilus assembly protein TraH | traH |
| PWC47_RS00575 | 117044..117472 | - | 429 | WP_004152689 | hypothetical protein | - |
| PWC47_RS00580 | 117465..118037 | - | 573 | WP_004152688 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PWC47_RS00585 | 118009..118248 | - | 240 | WP_004152687 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PWC47_RS00590 | 118259..119011 | - | 753 | WP_004152686 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PWC47_RS00595 | 119032..119358 | - | 327 | WP_004152685 | hypothetical protein | - |
| PWC47_RS00915 | 119404..119589 | - | 186 | WP_004152684 | hypothetical protein | - |
| PWC47_RS00600 | 119586..119822 | - | 237 | WP_004152683 | conjugal transfer protein TrbE | - |
| PWC47_RS00605 | 119854..121809 | - | 1956 | WP_004153093 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PWC47_RS00610 | 121857..122495 | - | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| PWC47_RS00615 | 122508..123467 | - | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| PWC47_RS00620 | 123511..124137 | - | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| PWC47_RS00625 | 124137..124526 | - | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
| PWC47_RS00630 | 124526..127165 | - | 2640 | WP_004152592 | type IV secretion system protein TraC | virb4 |
| PWC47_RS00635 | 127237..127635 | - | 399 | WP_004153071 | hypothetical protein | - |
| PWC47_RS00640 | 127931..128335 | - | 405 | WP_004152594 | hypothetical protein | - |
| PWC47_RS00645 | 128402..128719 | - | 318 | WP_004152595 | hypothetical protein | - |
| PWC47_RS00650 | 128720..128938 | - | 219 | WP_004171484 | hypothetical protein | - |
| PWC47_RS00655 | 128962..129252 | - | 291 | WP_004152596 | hypothetical protein | - |
| PWC47_RS00660 | 129257..129667 | - | 411 | WP_004152597 | lipase chaperone | - |
| PWC47_RS00665 | 129799..130368 | - | 570 | WP_004152598 | type IV conjugative transfer system lipoprotein TraV | traV |
| PWC47_RS00670 | 130391..130987 | - | 597 | WP_004152599 | conjugal transfer pilus-stabilizing protein TraP | - |
| PWC47_RS00675 | 130980..132404 | - | 1425 | WP_004152600 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PWC47_RS00680 | 132404..133144 | - | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| PWC47_RS00685 | 133131..133697 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| PWC47_RS00690 | 133717..134022 | - | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| PWC47_RS00695 | 134036..134404 | - | 369 | WP_004178060 | type IV conjugative transfer system pilin TraA | - |
| PWC47_RS00700 | 134458..134829 | - | 372 | WP_004208838 | TraY domain-containing protein | - |
| PWC47_RS00705 | 134913..135599 | - | 687 | WP_011977735 | transcriptional regulator TraJ family protein | - |
| PWC47_RS00710 | 135822..136214 | - | 393 | WP_004178062 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PWC47_RS00715 | 136648..137133 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| PWC47_RS00720 | 137166..137495 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| PWC47_RS00725 | 137528..138349 | - | 822 | WP_004178064 | DUF932 domain-containing protein | - |
| PWC47_RS00730 | 139183..139596 | - | 414 | WP_004152722 | helix-turn-helix domain-containing protein | - |
| PWC47_RS00735 | 139597..139875 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| PWC47_RS00740 | 139865..140185 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| PWC47_RS00745 | 140266..140481 | - | 216 | WP_077268719 | hypothetical protein | - |
| PWC47_RS00750 | 140501..140713 | - | 213 | WP_004152718 | hypothetical protein | - |
| PWC47_RS00755 | 140774..141130 | - | 357 | WP_004152717 | hypothetical protein | - |
| PWC47_RS00760 | 141765..142115 | - | 351 | WP_004152758 | hypothetical protein | - |
Host bacterium
| ID | 4620 | GenBank | NZ_OP378654 |
| Plasmid name | pKLP00172_2 | Incompatibility group | IncFIB |
| Plasmid size | 166588 bp | Coordinate of oriT [Strand] | 136526..136575 [+] |
| Host baterium | Klebsiella pneumoniae strain KLP00172 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |