Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104177 |
| Name | oriT_pKLP00161_2  |
| Organism | Klebsiella pneumoniae strain KLP00161 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OP378652 (213368..213417 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKLP00161_2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3070 | GenBank | WP_015065542 |
| Name | traI_PWC43_RS00965_pKLP00161_2 |
UniProt ID | K7R3U7 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190318.03 Da Isoelectric Point: 6.2890
>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2964 | GenBank | WP_015065541 |
| Name | traD_PWC43_RS00970_pKLP00161_2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86025.00 Da Isoelectric Point: 5.0577
>WP_015065541.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 183961..213975
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWC43_RS00970 | 183961..186273 | - | 2313 | WP_015065541 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PWC43_RS00975 | 186402..187091 | - | 690 | WP_015065540 | hypothetical protein | - |
| PWC43_RS00980 | 187283..188014 | - | 732 | WP_032329926 | conjugal transfer complement resistance protein TraT | - |
| PWC43_RS00985 | 188323..188847 | - | 525 | WP_015065538 | hypothetical protein | - |
| PWC43_RS00990 | 188858..191701 | - | 2844 | WP_023157999 | conjugal transfer mating-pair stabilization protein TraG | traG |
| PWC43_RS00995 | 191701..193080 | - | 1380 | WP_023158000 | conjugal transfer pilus assembly protein TraH | traH |
| PWC43_RS01000 | 193058..193501 | - | 444 | WP_023158001 | F-type conjugal transfer protein TrbF | - |
| PWC43_RS01005 | 193547..194104 | - | 558 | WP_023158002 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PWC43_RS01010 | 194076..194315 | - | 240 | WP_023158003 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PWC43_RS01015 | 194326..195078 | - | 753 | WP_015065535 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PWC43_RS01020 | 195099..195425 | - | 327 | WP_015065534 | hypothetical protein | - |
| PWC43_RS01025 | 195438..195686 | - | 249 | WP_004152675 | hypothetical protein | - |
| PWC43_RS01030 | 195664..195918 | - | 255 | WP_015065558 | conjugal transfer protein TrbE | - |
| PWC43_RS01035 | 195950..197905 | - | 1956 | WP_015065533 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PWC43_RS01040 | 197964..198611 | - | 648 | WP_015632510 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| PWC43_RS01045 | 198887..199876 | - | 990 | WP_015632509 | conjugal transfer pilus assembly protein TraU | traU |
| PWC43_RS01050 | 199873..200274 | - | 402 | WP_023158005 | hypothetical protein | - |
| PWC43_RS01055 | 200309..200944 | - | 636 | WP_023158006 | type-F conjugative transfer system protein TraW | traW |
| PWC43_RS01060 | 200944..201333 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| PWC43_RS01065 | 201333..203972 | - | 2640 | WP_023158007 | type IV secretion system protein TraC | virb4 |
| PWC43_RS01070 | 204044..204442 | - | 399 | WP_023158008 | hypothetical protein | - |
| PWC43_RS01075 | 204450..204839 | - | 390 | WP_023158009 | hypothetical protein | - |
| PWC43_RS01080 | 204882..205286 | - | 405 | WP_016831042 | hypothetical protein | - |
| PWC43_RS01085 | 205353..205664 | - | 312 | WP_014386200 | hypothetical protein | - |
| PWC43_RS01090 | 205665..205883 | - | 219 | WP_014386199 | hypothetical protein | - |
| PWC43_RS01095 | 205907..206196 | - | 290 | Protein_219 | hypothetical protein | - |
| PWC43_RS01275 | 206201..206611 | - | 411 | WP_016831039 | hypothetical protein | - |
| PWC43_RS01105 | 206743..207327 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| PWC43_RS01110 | 207350..207946 | - | 597 | Protein_222 | conjugal transfer pilus-stabilizing protein TraP | - |
| PWC43_RS01115 | 207939..209363 | - | 1425 | WP_023158013 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PWC43_RS01120 | 209363..210103 | - | 741 | WP_014386193 | type-F conjugative transfer system secretin TraK | traK |
| PWC43_RS01125 | 210090..210656 | - | 567 | WP_016831035 | type IV conjugative transfer system protein TraE | traE |
| PWC43_RS01130 | 210676..210981 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| PWC43_RS01135 | 210995..211363 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| PWC43_RS01140 | 211425..211631 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| PWC43_RS01145 | 211764..212483 | - | 720 | WP_016831034 | conjugal transfer protein TrbJ | - |
| PWC43_RS01150 | 212683..213099 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PWC43_RS01155 | 213490..213975 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| PWC43_RS01160 | 214008..214337 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| PWC43_RS01165 | 214370..215191 | - | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| PWC43_RS01170 | 216019..216582 | - | 564 | WP_004182054 | methyltransferase | - |
| PWC43_RS01175 | 216630..217985 | - | 1356 | WP_004182052 | DUF3560 domain-containing protein | - |
| PWC43_RS01180 | 218037..218267 | - | 231 | WP_001568051 | hypothetical protein | - |
| PWC43_RS01185 | 218359..218586 | - | 228 | WP_004182050 | hypothetical protein | - |
Host bacterium
| ID | 4616 | GenBank | NZ_OP378652 |
| Plasmid name | pKLP00161_2 | Incompatibility group | IncFIB |
| Plasmid size | 236466 bp | Coordinate of oriT [Strand] | 213368..213417 [+] |
| Host baterium | Klebsiella pneumoniae strain KLP00161 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15, aac(6')-Ib-cr, blaOXA-1, tet(A), qnrB1, dfrA14 |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR, fecD, fecE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |