Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104176 |
| Name | oriT_pKLP00149_2  |
| Organism | Klebsiella pneumoniae strain KLP00149 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OP378647 (117942..117990 [+], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_pKLP00149_2
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3069 | GenBank | WP_022644926 |
| Name | traI_PWC46_RS00530_pKLP00149_2 |
UniProt ID | W8RBA8 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190574.57 Da Isoelectric Point: 6.3191
>WP_022644926.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDRDGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKARSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLPPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDRDGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKARSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLPPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 1330 | GenBank | WP_022644939 |
| Name | WP_022644939_pKLP00149_2 |
UniProt ID | W8QGP1 |
| Length | 130 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 130 a.a. Molecular weight: 15048.16 Da Isoelectric Point: 4.5248
>WP_022644939.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEVAEKINAIVEKRRVEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LEYVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMLRFFPDEDNEVEE
MAKVQAYVSDEVAEKINAIVEKRRVEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LEYVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMLRFFPDEDNEVEE
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | W8QGP1 |
T4CP
| ID | 2963 | GenBank | WP_032072078 |
| Name | traD_PWC46_RS00535_pKLP00149_2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85822.78 Da Isoelectric Point: 4.9615
>WP_032072078.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 88843..115565
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWC46_RS00535 | 88843..91155 | - | 2313 | WP_032072078 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PWC46_RS00540 | 91284..91973 | - | 690 | WP_022644928 | hypothetical protein | - |
| PWC46_RS00545 | 92165..92896 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| PWC46_RS00550 | 93080..93628 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| PWC46_RS00555 | 93641..94246 | - | 606 | WP_019405095 | hypothetical protein | traG |
| PWC46_RS00560 | 94325..95022 | + | 698 | WP_095033700 | IS1-like element IS1B family transposase | - |
| PWC46_RS00565 | 95039..97267 | - | 2229 | Protein_113 | conjugal transfer mating-pair stabilization protein TraG | - |
| PWC46_RS00570 | 97267..98646 | - | 1380 | WP_073546760 | conjugal transfer pilus assembly protein TraH | traH |
| PWC46_RS00575 | 98624..99067 | - | 444 | WP_004194305 | F-type conjugal transfer protein TrbF | - |
| PWC46_RS00580 | 99113..99670 | - | 558 | WP_022644930 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PWC46_RS00585 | 99642..99881 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PWC46_RS00590 | 99892..100644 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PWC46_RS00595 | 100665..100991 | - | 327 | WP_004152676 | hypothetical protein | - |
| PWC46_RS00600 | 101004..101252 | - | 249 | WP_022644931 | hypothetical protein | - |
| PWC46_RS00605 | 101230..101484 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| PWC46_RS00610 | 101516..103471 | - | 1956 | WP_022644932 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PWC46_RS00615 | 103530..104168 | - | 639 | WP_022644933 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| PWC46_RS00620 | 104181..105170 | - | 990 | WP_015632509 | conjugal transfer pilus assembly protein TraU | traU |
| PWC46_RS00625 | 105167..105568 | - | 402 | WP_022644934 | hypothetical protein | - |
| PWC46_RS00630 | 105603..106238 | - | 636 | WP_020325113 | type-F conjugative transfer system protein TraW | traW |
| PWC46_RS00635 | 106235..106627 | - | 393 | WP_022644935 | type-F conjugative transfer system protein TrbI | - |
| PWC46_RS00640 | 106627..109266 | - | 2640 | WP_022644936 | type IV secretion system protein TraC | virb4 |
| PWC46_RS00645 | 109338..109736 | - | 399 | WP_011977783 | hypothetical protein | - |
| PWC46_RS00650 | 109744..110133 | - | 390 | WP_004153076 | hypothetical protein | - |
| PWC46_RS00655 | 110176..110580 | - | 405 | WP_004152503 | hypothetical protein | - |
| PWC46_RS00660 | 110647..110958 | - | 312 | WP_004152502 | hypothetical protein | - |
| PWC46_RS00665 | 110959..111177 | - | 219 | WP_004152501 | hypothetical protein | - |
| PWC46_RS00670 | 111283..111693 | - | 411 | WP_004152499 | hypothetical protein | - |
| PWC46_RS00675 | 111825..112409 | - | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| PWC46_RS00680 | 112523..113947 | - | 1425 | WP_022644937 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PWC46_RS00685 | 113947..114687 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| PWC46_RS00690 | 114674..115240 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| PWC46_RS00695 | 115260..115565 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| PWC46_RS00700 | 115579..115947 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| PWC46_RS00705 | 116016..116216 | - | 201 | WP_032072063 | TraY domain-containing protein | - |
| PWC46_RS00710 | 116301..117002 | - | 702 | WP_022644938 | hypothetical protein | - |
| PWC46_RS00715 | 117237..117629 | - | 393 | WP_022644939 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PWC46_RS00720 | 118135..118557 | + | 423 | WP_072205880 | transglycosylase SLT domain-containing protein | - |
| PWC46_RS00725 | 118581..119123 | - | 543 | WP_169319783 | antirestriction protein | - |
| PWC46_RS00730 | 119472..119708 | - | 237 | Protein_146 | SAM-dependent DNA methyltransferase | - |
| PWC46_RS00735 | 119756..119902 | - | 147 | WP_022644943 | hypothetical protein | - |
| PWC46_RS00740 | 119995..120342 | - | 348 | WP_022644944 | hypothetical protein | - |
Host bacterium
| ID | 4615 | GenBank | NZ_OP378647 |
| Plasmid name | pKLP00149_2 | Incompatibility group | - |
| Plasmid size | 165220 bp | Coordinate of oriT [Strand] | 117942..117990 [+] |
| Host baterium | Klebsiella pneumoniae strain KLP00149 |
Cargo genes
| Drug resistance gene | dfrA14, sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1A, blaOXA-9, aac(6')-Ib, blaKPC-3, blaSHV-182 |
| Virulence gene | - |
| Metal resistance gene | ncrA, ncrB, ncrC, ncrY |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |