Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104168 |
| Name | oriT_pKLP00215_4  |
| Organism | Klebsiella pneumoniae strain KLP00215 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OP378662 (89198..89247 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKLP00215_4
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3061 | GenBank | WP_004153015 |
| Name | traI_PWC31_RS00310_pKLP00215_4 |
UniProt ID | A0A8A5TN24 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190492.51 Da Isoelectric Point: 6.3661
>WP_004153015.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2950 | GenBank | WP_004152620 |
| Name | traD_PWC31_RS00315_pKLP00215_4 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85871.84 Da Isoelectric Point: 5.0101
>WP_004152620.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60808..89805
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PWC31_RS00315 | 60808..63120 | - | 2313 | WP_004152620 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PWC31_RS00320 | 63249..63938 | - | 690 | WP_004198206 | hypothetical protein | - |
| PWC31_RS00325 | 64130..64861 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| PWC31_RS00330 | 65045..65593 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| PWC31_RS00335 | 65606..68455 | - | 2850 | WP_004152624 | conjugal transfer mating-pair stabilization protein TraG | traG |
| PWC31_RS00340 | 68455..69834 | - | 1380 | WP_004165169 | conjugal transfer pilus assembly protein TraH | traH |
| PWC31_RS00345 | 69812..70255 | - | 444 | WP_004152679 | F-type conjugal transfer protein TrbF | - |
| PWC31_RS00350 | 70301..70858 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PWC31_RS00355 | 70830..71069 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PWC31_RS00360 | 71080..71832 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PWC31_RS00365 | 71853..72179 | - | 327 | WP_004152676 | hypothetical protein | - |
| PWC31_RS00370 | 72192..72440 | - | 249 | WP_004152675 | hypothetical protein | - |
| PWC31_RS00375 | 72418..72672 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| PWC31_RS00380 | 72704..74659 | - | 1956 | WP_004152673 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PWC31_RS00385 | 74718..75356 | - | 639 | WP_004152672 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| PWC31_RS00390 | 75369..76358 | - | 990 | WP_011977785 | conjugal transfer pilus assembly protein TraU | traU |
| PWC31_RS00395 | 76355..76744 | - | 390 | WP_004152508 | hypothetical protein | - |
| PWC31_RS00400 | 76786..77412 | - | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| PWC31_RS00405 | 77412..77801 | - | 390 | WP_004152506 | type-F conjugative transfer system protein TrbI | - |
| PWC31_RS00410 | 77801..80440 | - | 2640 | WP_004152505 | type IV secretion system protein TraC | virb4 |
| PWC31_RS00415 | 80512..80910 | - | 399 | WP_011977783 | hypothetical protein | - |
| PWC31_RS00420 | 80918..81307 | - | 390 | WP_004153076 | hypothetical protein | - |
| PWC31_RS00425 | 81350..81754 | - | 405 | WP_004152503 | hypothetical protein | - |
| PWC31_RS00430 | 81821..82132 | - | 312 | WP_004152502 | hypothetical protein | - |
| PWC31_RS00435 | 82133..82351 | - | 219 | WP_004152501 | hypothetical protein | - |
| PWC31_RS00440 | 82457..82867 | - | 411 | WP_004152499 | hypothetical protein | - |
| PWC31_RS00445 | 82999..83583 | - | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| PWC31_RS00450 | 83697..85121 | - | 1425 | WP_004155033 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PWC31_RS00455 | 85121..85861 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| PWC31_RS00460 | 85848..86414 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| PWC31_RS00465 | 86434..86739 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| PWC31_RS00470 | 86753..87121 | - | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| PWC31_RS00475 | 87175..87561 | - | 387 | WP_004152495 | TraY domain-containing protein | - |
| PWC31_RS00480 | 87640..88326 | - | 687 | WP_004152494 | transcriptional regulator TraJ family protein | - |
| PWC31_RS00485 | 88500..88898 | - | 399 | WP_004152493 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PWC31_RS00490 | 89320..89805 | + | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| PWC31_RS00495 | 89838..90167 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| PWC31_RS00500 | 90200..91021 | - | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| PWC31_RS00505 | 91841..92674 | - | 834 | WP_004152751 | N-6 DNA methylase | - |
| PWC31_RS00510 | 92725..92871 | - | 147 | WP_004152750 | hypothetical protein | - |
| PWC31_RS00515 | 92966..93313 | - | 348 | WP_004152749 | hypothetical protein | - |
| PWC31_RS00520 | 93370..93723 | - | 354 | WP_004152748 | hypothetical protein | - |
| PWC31_RS00525 | 94353..94703 | - | 351 | WP_004153414 | hypothetical protein | - |
Host bacterium
| ID | 4607 | GenBank | NZ_OP378662 |
| Plasmid name | pKLP00215_4 | Incompatibility group | IncFIB |
| Plasmid size | 113637 bp | Coordinate of oriT [Strand] | 89198..89247 [+] |
| Host baterium | Klebsiella pneumoniae strain KLP00215 |
Cargo genes
| Drug resistance gene | blaTEM-1A, blaOXA-9, blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |