Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104128 |
| Name | oriT_2022CK-00757|unnamed2  |
| Organism | Enterobacter asburiae strain 2022CK-00757 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP117725 (3691..3801 [-], 111 nt) |
| oriT length | 111 nt |
| IRs (inverted repeats) | 71..78, 81..88 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 111 nt
>oriT_2022CK-00757|unnamed2
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 3035 | GenBank | WP_054471492 |
| Name | Relaxase_PTC87_RS23700_2022CK-00757|unnamed2 |
UniProt ID | _ |
| Length | 610 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 610 a.a. Molecular weight: 66683.43 Da Isoelectric Point: 10.6436
>WP_054471492.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICDGLGLGEHQRVSAVHNDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCTTLERDYGLERDNHESRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQAAPARRNYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRRKDRAIENAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQATAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHT
PVIDSITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRLGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPAQAP
TQATPTPPPAKVQQPAKPAPSELEQRQARLRAQMEAKKAKRQDKGKGRGR
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICDGLGLGEHQRVSAVHNDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCTTLERDYGLERDNHESRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQAAPARRNYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRRKDRAIENAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQATAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHT
PVIDSITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRLGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPAQAP
TQATPTPPPAKVQQPAKPAPSELEQRQARLRAQMEAKKAKRQDKGKGRGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 1302 | GenBank | WP_054471493 |
| Name | WP_054471493_2022CK-00757|unnamed2 |
UniProt ID | A0A120LKZ5 |
| Length | 125 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 125 a.a. Molecular weight: 13520.32 Da Isoelectric Point: 7.9653
>WP_054471493.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Pseudomonadota]
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGQS
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGQS
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A120LKZ5 |
Host bacterium
| ID | 4567 | GenBank | NZ_CP117725 |
| Plasmid name | 2022CK-00757|unnamed2 | Incompatibility group | - |
| Plasmid size | 33750 bp | Coordinate of oriT [Strand] | 3691..3801 [-] |
| Host baterium | Enterobacter asburiae strain 2022CK-00757 |
Cargo genes
| Drug resistance gene | blaOXA-2, aac(6')-IIc, qacE, sul1, blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | merD, merA, merF, merP, merT, merR2 |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |