Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104119
Name   oriT_2022CK-00781|unnamed2 in_silico
Organism   Klebsiella grimontii strain 2022CK-00781
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP117732 (64630..64780 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_2022CK-00781|unnamed2
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3031 GenBank   WP_001337757
Name   mobH_PTC90_RS30025_2022CK-00781|unnamed2 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   2920 GenBank   WP_000178856
Name   traD_PTC90_RS30030_2022CK-00781|unnamed2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   2921 GenBank   WP_273863456
Name   traC_PTC90_RS30110_2022CK-00781|unnamed2 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92574.87 Da        Isoelectric Point: 5.9222

>WP_273863456.1 type IV secretion system protein TraC [Klebsiella grimontii]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVFSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraG P-loop domain (P-loop_TraG)

(449-809)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62714..87905

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTC90_RS30015 (PTC90_30010) 58739..58972 - 234 WP_001191892 hypothetical protein -
PTC90_RS30020 (PTC90_30015) 58954..59571 - 618 WP_001249396 hypothetical protein -
PTC90_RS30025 (PTC90_30020) 59739..62717 + 2979 WP_001337757 MobH family relaxase -
PTC90_RS30030 (PTC90_30025) 62714..64579 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
PTC90_RS30035 (PTC90_30030) 64629..65174 + 546 WP_000228720 hypothetical protein -
PTC90_RS30040 (PTC90_30035) 65131..65760 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
PTC90_RS30045 (PTC90_30040) 65770..66216 + 447 WP_000122506 hypothetical protein -
PTC90_RS30050 (PTC90_30045) 66226..66603 + 378 WP_000869261 hypothetical protein -
PTC90_RS30055 (PTC90_30050) 66603..67265 + 663 WP_001231463 hypothetical protein -
PTC90_RS30060 (PTC90_30055) 67446..67589 + 144 WP_001275801 hypothetical protein -
PTC90_RS30065 (PTC90_30060) 67601..67966 + 366 WP_001052531 hypothetical protein -
PTC90_RS30070 (PTC90_30065) 68111..68392 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
PTC90_RS30075 (PTC90_30070) 68389..69015 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
PTC90_RS30080 (PTC90_30075) 68999..69916 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
PTC90_RS30085 (PTC90_30080) 69916..71229 + 1314 WP_024131605 TraB/VirB10 family protein traB
PTC90_RS30090 (PTC90_30085) 71226..71804 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
PTC90_RS30095 (PTC90_30090) 71808..72200 + 393 WP_000479535 TraA family conjugative transfer protein -
PTC90_RS30100 (PTC90_30095) 72401..77932 + 5532 WP_000606833 Ig-like domain-containing protein -
PTC90_RS30105 (PTC90_30100) 78081..78788 + 708 WP_001259347 DsbC family protein -
PTC90_RS30110 (PTC90_30105) 78785..81232 + 2448 WP_273863456 type IV secretion system protein TraC virb4
PTC90_RS30115 (PTC90_30110) 81247..81564 + 318 WP_000351984 hypothetical protein -
PTC90_RS30120 (PTC90_30115) 81561..82091 + 531 WP_001010738 S26 family signal peptidase -
PTC90_RS30125 (PTC90_30120) 82054..83319 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
PTC90_RS30130 (PTC90_30125) 83316..83987 + 672 WP_001337754 EAL domain-containing protein -
PTC90_RS30135 (PTC90_30130) 83987..84991 + 1005 WP_043940352 TraU family protein traU
PTC90_RS30140 (PTC90_30135) 85107..87905 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
PTC90_RS30145 (PTC90_30140) 87944..88804 - 861 WP_000709517 hypothetical protein -
PTC90_RS30150 (PTC90_30145) 88927..89568 - 642 WP_000796664 hypothetical protein -
PTC90_RS30155 (PTC90_30150) 89862..90182 + 321 WP_000547566 hypothetical protein -
PTC90_RS30160 (PTC90_30155) 90486..90671 + 186 WP_001186917 hypothetical protein -
PTC90_RS30165 (PTC90_30160) 90891..91859 + 969 WP_000085162 AAA family ATPase -
PTC90_RS30170 (PTC90_30165) 91870..92778 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   4558 GenBank   NZ_CP117732
Plasmid name   2022CK-00781|unnamed2 Incompatibility group   IncA/C2
Plasmid size   178660 bp Coordinate of oriT [Strand]   64630..64780 [+]
Host baterium   Klebsiella grimontii strain 2022CK-00781

Cargo genes

Drug resistance gene   blaFOX-5, blaCARB-2, aadA2, cmlA1, qacE, sul1, dfrA19, ant(2'')-Ia, msr(E)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -