Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104112
Name   oriT_pEA22_6 in_silico
Organism   Escherichia albertii strain BIA_22
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP117634 (1935..2091 [-], 157 nt)
oriT length   157 nt
IRs (inverted repeats)      101..108, 118..125  (ACAAACTC..GAGTTTGT)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 157 nt

>oriT_pEA22_6
GTAACTCCGTGCGCGGTGAAAAATCGCATTTTAGCGCGTCACTGGTAGTTTAAAAACTGAACTGGCATAATGCACAGCAAATCACGAAGTGCGCACTTATACAAACTCTCCTTCGTCGAGTTTGTGTGCGCCCTGCGGGGCTAAAAGAAAACGGCAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3028 GenBank   WP_024236174
Name   mobQ_PS041_RS26620_pEA22_6 insolico UniProt ID   A0A3S7R4T3
Length   715 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 715 a.a.        Molecular weight: 81916.26 Da        Isoelectric Point: 7.0742

>WP_024236174.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRKNGVASAVILTPANAPAWCANRAELW
NAVEKAERRKNSQLAREFELAIPRELAQDAARETVLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSM
RAITPDGFGEKVREWNDWTHAETWRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAM
EKWGIETERGEQNRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDS
NAAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWVAPEYGREQEK
IQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQLRKREEELRRKEQEETERRRAE
QLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRARIFESVDLMRDR
SGYICERSEIGQRPPLQHVYSSATFEQLLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARK
VDFTQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDK
GLRFAGYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEWQRDEYA
RTHATERRHDRGWSQ

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-212)


  Protein structure


Source ID Structure
AlphaFold DB A0A3S7R4T3


Host bacterium

ID   4551 GenBank   NZ_CP117634
Plasmid name   pEA22_6 Incompatibility group   Col8282
Plasmid size   4091 bp Coordinate of oriT [Strand]   1935..2091 [-]
Host baterium   Escherichia albertii strain BIA_22

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -